Identification and Classification of Main Iranian Olive Cultivars Using Microsatellite Markers

Abstract

<jats:p>Numerous olive cultivars are cultivated in Iran, mainly in the north. Ninety-two accessions belonging to 10 main olive cultivars were screened by 13 microsatellite markers revealing high genetic variability both within and between cultivars. In total, 72 alleles were detected with a mean number of 5.5 alleles per locus. Twenty-four unique allelic patterns were observed, whereas six genotypes showed 15 unique alleles. Heterozygosity ranged from 0.00 to 0.98, whereas the mean number of discrimination power and polymorphic information content were 0.55 and 0.54, respectively. The combination of 5 simple sequence repeat markers made discrimination of 84% of all accessions included in the study possible. The existence of homonyms, synonyms, or mislabeling as well as intracultivar polymorphism was revealed by allele differences between accessions of the same denomination. The phenogram showed variability among as well as between some cultivars, but most accessions with the same generic names were grouped together.</jats:p>

Assessment of genetic diversity among Algerian olive (Olea europaea L.) cultivars using SSR marker.

“…Both He and PD values revealed for each primer a high genetic variability and discrimination efficiency among genotypes. The same finding were reported in others studies (Besnard et al, 2001;Noormohammad et al, 2007;Bracci et al, 2009;Alba et al, 2009). However, GAPU 59 showed he lowest number of alleles, the lower DC value and the lowest PD value.…”

Microsatellite marker-based identification of mother plants for the reliable propagation of olive (Olea europaeaL.) cultivars in Australia

“…They may also reflect different provenances and/or errors in labelling or propagation of olive plant material. Our results reconfirm the importance of the use of molecular markers for the correct management of olive collections, as shown previously (Belaj et al, 2003a,b;Noormohammadi et al, 2007). However, the most striking result here, compared with previous work on olive cultivar identification, was the use of a set of microsatellite markers to characterise mother trees that had been used extensively for the commercial propagation of olive varieties in Australia.…”

“…The allelic profiles of only three highly polymorphic microsatellite loci (DCA9, EM030, and DCA18) allowed all 53 samples to be assigned to each of the 23 genotypes (Table II), suggesting that these microsatellite markers can be used as diagnostic markers. These results reconfirmed the discriminatory capacity of microsatellite markers for characterising different olive cultivars, as reported previously (Belaj et al, 2003a;Noormohammadi et al, 2007;Bracci et al, 2009). At the individual level, 20 out of the 53 tree samples (37.7%) correctly matched their presumed cultivar identity.…”

“…Various molecular markers systems such as Randomly Amplified Polymorphic DNA (RAPD) and microsatellite markers have been used internationally to characterise olive cultivars (Belaj et al, 2001;2003a;Bandelj et al, 2007;Bracci et al, 2009;Mekuria et al, 1999;Guerin et al, 2002;Mailer and May., 2002). In recent years, microsatellites have been recognised as the markers of choice for the discrimination of cultivars and the assignment of cultivars to their geographic origin, as well as providing data for various other molecular analyses (Sarri et al, 2006;Noormohammadi et al, 2007;Poljuha et al, 2008;Muzzalupo et al, 2009). Microsatellite markers are particularly suited to these analyses as they are highly polymorphic, amenable to detecting heterozygosity, and highly reproducible (Belaj et al, 2003a;Baldoni et al, 2009).…”

The coexistence of oleaster and traditional varieties affects genetic diversity and population structure in Algerian olive (Olea europaea) germplasm

“…Moreover, this germplasm is named after geographic, agronomic, or morphological traits (Hauville 1953;Mendil and Sebai 2006), such as fruit form (e.g., ''Tefah'' for apple, and ''Tabelout'' for acorn), or their site of origin (e.g., 'Takesrit, for El-Kseur on the valley of Soummam). Thus, confusing names are very common (Noormohammadi et al 2007;Muzzalupo et al 2009). In our study, we found clear similarity between two different traditional varieties, Agrarez_IT and Azeradj_IT, suggesting a putative case of synonymy.…”