The coexistence of oleaster and traditional varieties affects genetic diversity and population structure in Algerian olive (Olea europaea) germplasm

Boucheffa, Saliha; Miazzi, Monica Marilena; Rienzo, Valentina di; Mangini, Giacomo; Fanelli, Valentina; Tamendjari, Abderezak; Pignone, Domenico; Montemurro, Cinzia

doi:10.1007/s10722-016-0365-4

Cited by 33 publications

(54 citation statements)

References 44 publications

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“…Today, there is a strong interest in the recovery and preservation of agro-biodiversity, and several projects are in place to avoid the loss of this patrimony, setting up recovery collections [32][33][34][35]. In Tunisia, although there are institutions, such as the Olive Institute, that hold a large number of olive genotypes, it is still necessary to enlarge the existing collection and develop new conservation management strategies [36][37][38]. At the same time, it is crucial to improve the plant material certification circuit to be more competitive in the global market, offering plant material with high quality standards [39].…”

Section: Discussionmentioning

confidence: 99%

Recovery, Assessment, and Molecular Characterization of Minor Olive Genotypes in Tunisia

Debbabi

Miazzi

Elloumi³

et al. 2020

Plants

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Olive is one of the oldest cultivated species in the Mediterranean Basin, including Tunisia, where it has a wide diversity, with more than 200 cultivars, of both wild and feral forms. Many minor cultivars are still present in marginal areas of Tunisia, where they are maintained by farmers in small local groves, but they are poorly characterized and evaluated. In order to recover this neglected germplasm, surveys were conducted in different areas, and 31 genotypes were collected, molecularly characterized with 12 nuclear microsatellite (simple sequence repeat (SSR)) markers, and compared with 26 reference cultivars present in the Tunisian National Olive collection. The analysis revealed an overall high genetic diversity of this olive’s germplasm, but also discovered the presence of synonymies and homonymies among the commercialized varieties. The structure analysis showed the presence of different gene pools in the analyzed germplasm. In particular, the marginal germplasm from Ras Jbal and Azmour is characterized by gene pools not present in commercial (Nurseries) varieties, pointing out the very narrow genetic base of the commercialized olive material in Tunisia, and the need to broaden it to avoid the risk of genetic erosion of this species in this country.

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Section: Discussionmentioning

confidence: 99%

Recovery, Assessment, and Molecular Characterization of Minor Olive Genotypes in Tunisia

Debbabi

Miazzi

Elloumi³

et al. 2020

Plants

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“…In accordance to previous studies, ( Baldoni et al, 2006 ; Hannachi et al, 2008 ; Belaj et al, 2010 ; Mousavi et al, 2017a ), the EST-SNPs displayed a high genetic differentiation between wild and cultivated forms. Besides, the detection of a clear wild genetic background for some cultivars such as ‘Dokkar,’ ‘Chemlal de Kabilye’ and ‘Sivigliana da Olio,’ supports the introgression of local wild gene pools into domesticated olives ( Belaj et al, 2010 ; Erre et al, 2010 ; Boucheffa et al, 2016 ; Mousavi et al, 2017a ). At the same time, the similarity found between some wild and cultivated genotypes under study, may indicate a possible feral origin of some uncultivated genotypes ( Belaj et al, 2010 ).…”

Section: Discussionmentioning

confidence: 90%

Usefulness of a New Large Set of High Throughput EST-SNP Markers as a Tool for Olive Germplasm Collection Management

et al. 2018

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Germplasm collections are basic tools for conservation, characterization, and efficient use of olive genetic resources. The identification of the olive cultivars maintained in the collections is an important ongoing task which has been performed by both, morphological and molecular markers. In the present study, based on the sequencing results of previous genomic projects, a new set of 1,043 EST-SNP markers has been identified. In order to evaluate its discrimination capacity and utility in diversity studies, this set of markers was used in a representative number of accessions from 20 different olive growing countries and maintained at the World Olive Germplasm Collection of IFAPA Centre ‘Alameda del Obispo’ (Córdoba, Spain), one of the world’s largest olive germplasm bank. Thus, the cultivated material included: cultivars belonging to previously defined core collections by means of SSR markers and agronomical traits, well known homonymy cases, possible redundancies previously identified in the collection, and recently introduced accessions. Marker stability was tested in repeated analyses of a selected number of accessions, as well as in different trees and accessions belonging to the same cultivar. In addition, 15 genotypes from a cross ‘Picual’ × ‘Arbequina’ cultivars from the IFAPA olive breeding program and a set of 89 wild genotypes were also included in the study. Our results indicate that, despite their relatively wide variability, the new set of EST-SNPs displayed lower levels of genetic diversity than SSRs in the set of olive core collections tested. However, the EST-SNP markers displayed consistent and reliable results from different plant material sources and plant propagation events. The EST-SNPs revealed a clear cut off between inter- and intra-cultivar variation in olive. Besides, they were able to reliably discriminate among different accessions, to detect possible homonymy cases as well as efficiently ascertain the presence of redundant germplasm in the collection. Additionally, these markers were highly transferable to the wild genotypes. These results, together with the low genotyping error rates and the easy and fully automated procedure used to get the genotyping data, validate the new set of EST-SNPs as possible markers of choice for olive cultivar identification.

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“…The presence of private alleles could reflect the genetic diversity of the germplasm to some extent and facilitates the identification of accessions (Mariotti et al, 2016;Boucheffa et al, 2017), which would be valuable in future breeding endeavors (Boucheffa et al, 2017). In this study, private alleles were found in 15 olive accessions, of which ''Yunza No.1'',''Yunza No.2'',''Yunza No.3'' from China contained more private alleles than the other 12 accessions (Table 4).…”

Section: Discussionmentioning

confidence: 99%

“…The rich diversity of this species is a consequence of its allogamous nature, a remarkable tree longevity, multiple domestication events such as crosses among cultivars and local selection, as well as a lack of turnover with new breeding genotypes (Díez et al, 2015;Belaj et al, 2016;Besnard, Terral & Cornille, 2018). Since this rich germplasm represents a source of valuable traits, the identification and characterization of olive cultivars and unknown accessions is firstly required for better exploiting and protecting olive resources as well as designing breeding programs (Boucheffa et al, 2017;Cultrera et al, 2019). During the long-term cultivation of olive, wrong naming of cultivars such as homonymy or synonymy and mistakes in labeling and propagation of cultivars have often led to misleading classification and misinterpreting relationships among cultivars (Beghè et al, 2015;Mariotti et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The newly developed genomic-SSR markers uncover the genetic characteristics and relationships of olive accessions

Cui

Pang

et al. 2020

PeerJ

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Background Olive (Olea europaea L.) is an important oil and fruit crop worldwide, owning a rich germplasm with a large number of cultivars. Simple sequence repeats (SSRs) are excellent markers and have been used for the identification of olive cultivars. However, the limited number of SSR markers and the occurrence of confusion on the names of cultivars, as well as the possible appearance of clonal variation make it difficult to identify cultivars and interpret relationships among olive cultivars. Method SSR markers were designed based on trinucleotide repeat sequences by screening the whole genome of olive, and the polymorphic SSR markers were developed that were applied to the identification of 53 olive accessions. The genetic characteristics and relationships of these olive accessions were evaluated based on the developed SSR markers. Results Twenty-one highly polymorphic genomic-SSR markers were developed, covering most chromosomes of olive. These SSR markers could well distinguish all 53 olive accessions, confirming their effectiveness. DNA fingerprints of the 53 olive accessions were constructed based on the 21 SSR markers. The dendrogram clearly divided the tested accessions into two main groups, which was also supported by the results of principal coordinate analysis. A total of 31 private alleles were detected in 15 olive accessions, which reflected the genetic diversity within 53 olive accessions to some extent. Six homonymy cases were also clarified by genetic analysis. These results suggest that the newly developed olive SSR markers are informative for the exploitation, preservation and breeding of olive.

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The coexistence of oleaster and traditional varieties affects genetic diversity and population structure in Algerian olive (Olea europaea) germplasm

Cited by 33 publications

References 44 publications

Recovery, Assessment, and Molecular Characterization of Minor Olive Genotypes in Tunisia

Recovery, Assessment, and Molecular Characterization of Minor Olive Genotypes in Tunisia

Usefulness of a New Large Set of High Throughput EST-SNP Markers as a Tool for Olive Germplasm Collection Management

The newly developed genomic-SSR markers uncover the genetic characteristics and relationships of olive accessions

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