Identification and Application of BhAPRR2 Controlling Peel Colour in Wax Gourd (Benincasa hispida)

Ma, Lianlian; Liu, Zhengguo; Cheng, Zhikui; Gou, Jiquan; Chen, Jieying; Yu, Weiwu; Wang, Peng

doi:10.3389/fpls.2021.716772

Cited by 27 publications

(34 citation statements)

References 56 publications

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“…The InDel marker loci were screened based on previously obtained resequencing data and gene mapping results for two wax gourd populations (one cylindrical with green peel and the other nearly spherical with white peel) belonging to our research group [ 17 , 18 ]. GatK4 software [ 19 ] was used to analyse InDel loci between these two wax gourd populations according to effective resequencing data and the wax gourd reference genome.…”

Section: Methodsmentioning

confidence: 99%

“…To date, InDel markers have been widely developed and utilised in the breeding of a variety of vegetables and fruit such as watermelon [ 11 ], cabbage [ 12 ], cucumber [ 13 ], tomato [ 14 ], Chinese cabbage [ 15 ], and rape [ 16 ]. Previously, our research group fine-mapped the gene that regulates fruit shape ( Bch02G016830 ) in the 19.6 Kb region of chromosome 2 [ 17 ] and the gene that regulates peel colour ( Bch05G003950 ) in the 179 Kb region of chromosome 5 [ 18 ]. The fine mapping and cloning of these two genes laid a foundation for the subsequent development of InDel markers for the assisted breeding of wax gourds.…”

Section: Introductionmentioning

confidence: 99%

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Development and Application of InDel Markers Linked to Fruit-Shape and Peel-Colour Genes in Wax Gourd

Huang

et al. 2022

Genes

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The wax gourd is commonly grown in many countries because of its high nutritional and economic value. While the genes for the fruit shape and peel colour of wax gourd have been reported, the InDel markers linked to these genes remain undeveloped. In this study, the InDel markers linked to fruit-shape (Bch02G016830) and peel-colour (Bch05G003950) genes were developed from resequenced data. We used 120 inbred lines, 536 isolated populations, and 4 commercial hybrids to evaluate the validity and application value of the InDel markers. The accuracy rates of nine pairs of fruit-shape InDel markers (GX1-GX9) were 84.16–91.66% in 120 inbred lines. The accuracy rates of 27 pairs of peel-colour InDel markers (PS1-PS27) within approximately 3.0 Mb upstream and 3.0 Mb downstream of the peel-colour gene were 100% and those of 6 pairs of peel-colour InDel markers (PS28-PS33) within 3.0–20 Mb upstream and downstream of the peel-colour gene were 55.83–90% in 120 inbred lines. The purity of four commercial hybrids determined using GX1, GX2, PS13, and PS14 was highly consistent with the field results for purity determination. Our results provide important information for genetic linkage map construction, molecular-marker-assisted selective breeding, and purity determination of wax gourd hybrids.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and Application of InDel Markers Linked to Fruit-Shape and Peel-Colour Genes in Wax Gourd

Huang

et al. 2022

Genes

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“…The fruits were collected at different developmental stages (0, 5,10,15,20,25,30,35 and 40 DAP). Their pericarps were longitudinally cut into slices about 1 cm wide and 0.2 cm thick with a razor blade, and then ground into powder using liquid nitrogen.…”

Section: Pigment Extraction and Measurementmentioning

confidence: 99%

“…Pericarp samples at different developmental stages (0, 5,10,15,20,25,30,35,40 DAP) and various tissue samples (root, stem, leaf, ower) were collected from the parent plants. Total RNA from the above materials was extracted using the Plant Total RNA Puri cation Kit (Eastep® Super, Beijing, China) according to the manufacturer's instructions.…”

Section: Rna Extraction and Gene Expression Analysismentioning

confidence: 99%

Fine mapping and identification of SmAPRR2 regulating rind color in eggplant (Solanum melongena L.)

Fang

Wang

Peng

et al. 2022

Preprint

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Rind color is an economically important agronomic trait in eggplant that impacts consumer preferences. However, the gene(s) regulating eggplant rind color have not been characterized. In this study, bulked segregant analysis (BSA) and kompetitive allele-specific PCR (KASP) were employed to identify a candidate gene for eggplant rind color through constructing an F2 population generated from a cross between 'BL01' (green pericarp) and 'B1' (white pericarp). Genetic analysis of rind color revealed that a single dominant gene controls green color of eggplant peel. Pigment content measurement and cytological observations demonstrated that chlorophyll and carotenoid content and the number of chloroplasts and thylakoids in BL01 were higher than in B1. Using a BSA-seq method and a recombinant mapping strategy in F2 plants, a candidate gene (EGP19168.1) was fine-mapped to a 20.36 Kb interval on chromosome 8. The gene is predicted to encode the two-component response regulator-like protein Arabidopsis pseudo-response regulator2 (APRR2). Subsequently, allelic sequence analysis revealed that a single-base deletion in white-skinned eggplant led to a premature termination codon. A credible molecular marker closely linked to the candidate gene was developed to genotype 45 diverse eggplant germplasms, which could predict the trait of rind color (green and white) with a 91.1% accuracy rate. This study will be valuable for molecular marker-assisted selection in eggplant breeding and provides theoretical foundation for analyzing the formation mechanism of eggplant peel color.

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“…Bulked segregant analysis (BSA) and QTL mapping with high-density genetic maps are effective methods to locate the main genetic regions of target traits, and are widely used in horticultural crops, such as watermelon [25], melon [26], peach [27], cabbage [28], and cucumber [29]. In wax gourd, the fine mapping of peel color and fruit shape candidate genes was completed via BSA [30,31]. The high-density genetic map of wax gourd constructed using SLAF-seq identified QTLs related to peel color, fruit weight, fruit length, fruit diameter, and flesh thickness [32,33].…”

Section: Introductionmentioning

confidence: 99%

Fine Mapping and Functional Analysis of Major Regulatory Genes of Soluble Solids Content in Wax Gourd (Benincasa hispida)

Wang

Huang

et al. 2022

IJMS

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Soluble solids content (SSC) is an important quality trait of wax gourd, but reports about its regulatory genes are scarce. In this study, the SSC regulatory gene BhSSC2.1 in wax gourd was mined via quantitative trait locus (QTL) mapping based on high-density genetic mapping containing 12 linkage groups (LG) and bulked segregant analysis (BSA)-seq. QTL mapping and BSA-seq revealed for the first time that the SSC QTL (107.658–108.176 cM) of wax gourd was on Chr2 (LG2). The interpretable phenotypic variation rate and maximum LOD were 16.033% and 6.454, respectively. The QTL interval contained 13 genes. Real-time fluorescence quantitative expression analysis, functional annotation, and sequence analysis suggested that Bch02G016960, named BhSSC2.1, was a candidate regulatory gene of the SSC in wax gourd. Functional annotation of this gene showed that it codes for a NADP-dependent malic enzyme. According to BhSSC2.1 sequence variation, an InDel marker was developed for molecular marker-assisted breeding of wax gourd. This study will lay the foundation for future studies regarding breeding and understanding genetic mechanisms of wax gourd.

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Identification and Application of BhAPRR2 Controlling Peel Colour in Wax Gourd (Benincasa hispida)

Cited by 27 publications

References 56 publications

Development and Application of InDel Markers Linked to Fruit-Shape and Peel-Colour Genes in Wax Gourd

Development and Application of InDel Markers Linked to Fruit-Shape and Peel-Colour Genes in Wax Gourd

Fine mapping and identification of SmAPRR2 regulating rind color in eggplant (Solanum melongena L.)

Fine Mapping and Functional Analysis of Major Regulatory Genes of Soluble Solids Content in Wax Gourd (Benincasa hispida)

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