<i>In Vitro</i>Antimicrobial Properties of Coconut Oil on<i>Candida</i>Species in Ibadan, Nigeria

Ogbolu, David Olusoga; Oni, A.A.; Daini, OA; Oloko, A.P.

doi:10.1089/jmf.2006.1209

Cited by 113 publications

(84 citation statements)

References 17 publications

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“…Consistent though was the inhibitory effect of lauric acids in all replicates confirming the assumption that it might be the principle responsible for the antibacterial effect of coconut oils (8,9,14). Table 2.…”

Section: Resultssupporting

confidence: 72%

“…Results from the LJ slants also showed the inhibitory effects of both VCO 012 and VCO 013 on H37RV. However, literatures dealing on the L-J medium reported some limitations using the media on recovering isolates of M. tuberculosis (14). For example, a study conducted in India in 2005 compared the biphasic system (Middlebrook 7H11 agar slant + Middlebrook 9H broth) with Lowenstein -Jensen (LJ) medium.…”

Section: Discussionmentioning

confidence: 99%

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Preliminary study on the In-Vitro Susceptibility of Mycobacterium tuberculosis Isolates to Virgin Coconut Oil

Dalmacion

Ortega

Pena

et al. 2012

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ABSTRACT:Background: Tuberculosis remains a major public health threat. Studies have shown that medium chain fatty acids (MCFA), such as, those found in Virgin Coconut Oil (VCO), possess activity against a wide range of microorganisms including Mycobacterium tuberculosis. The goal of the study was to determine the in-vitro susceptibility of M. tuberculosis (TB) isolates to two commercial brands of VCOs each containing different amounts of lauric acid and produced by two different extraction processes. Two brands of VCO were tested for their anti-TB actions compared to pure lauric acid and a negative control. Two replicate runs were done. VCO samples were inoculated with 0.1 mL of the H37RV strain of M. tuberculosis and incubated at 37 0 C with shaking for 5 days. After incubation, the mixtures were diluted 10-fold up to 10 -4concentration to obtain countable colonies using Middlebrook 7H10 agar plates and LowensteinJensen (L-J) slants. Colony growths of M. tuberculosis were examined in each dilution every week for three to eight weeks.Results: Both brands of VCO at increasing dilution from 1:10 to 1:10000 lowered the colony counts of M. tuberculosis by 46% to 100 % on the Middlebrook 7H10 agar. Similar results were noted on L-J slants with colony counts decreasing by 25% to 96%. Lauric acid solution showed complete inhibition of bacterial growth in both the agar plates and LJ slants. potential of VCOs agent against the growth of M. tuberculosis Similar effect on other organisms is a possibility and should also be explored. But more studies are needed to replicate the studies using different concentrations of VCOs, lauric acid and other fatty acids in VCO such as myristic acid, caproic or caprylic acids. Conclusion

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Section: Resultssupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Preliminary study on the In-Vitro Susceptibility of Mycobacterium tuberculosis Isolates to Virgin Coconut Oil

Dalmacion

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Pena

et al. 2012

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“…17 The minimum inhibitory concentration is the lowest concentration of virgin coconut oil that is needed to inhibit the growth of oral Candida albicans, which is 25%. 8 Once the minimum inhibitory concentration has been determined, the exposure time is determined. Exposure time is the minimum duration of time needed for virgin coconut oil to inhibit the growth of C. albicans.A test tube containing1.0 ml of the MIC of virgin coconut oil is added to 0.1 ml oral C. albicans suspension.…”

Section: Methodsmentioning

confidence: 99%

“…7 Virgin coconut oil has also been proven to have antifungal effects against clinical specimens of all Candida species. 8 Fatty acids are carboxylic acid with a long unbranched aliphatic tail,derived from or contained in an esterified form of an animal or vegetable fat, oil or wax, which may be saturated or unsaturated such as lauric acid, myristic acid, palmitic acid, capric acid, caprylic acid, oleic acid, stearic acid, linoleic acid, and caproic acid. 9 These fatty acids will be converted into their corresponding 1-monoglycerides by the body.…”

Section: Introductionmentioning

confidence: 99%

Exposure time of virgin coconut oil against oral Candida albicans

2016

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Introduction: Virgin coconut oil (VCO) is an oil which is purely made as a product of the coconut palm and has been used for centuries in the culinary industry and the medical field. Currently, VCO is used widely in medical field due to its effectiveness as antibacterial, antiviral, and antifungal properties. Objective of this experimental study was to prove the effectiveness of virgin coconut oil by determining its exposure time at concentration of 25% to inhibit growth of oral Candida albicans. Method: The research was done by quasi experiment design using the serial dilution method. The exposure time test was performed using a spread plate technique with virgin coconut oil at concentration of 25 % against oral Candida albicans isolated from saliva. Intervals of day 0, 1, 2, and 3 were used. Result: The result showed that virgin coconut oil inhibited the growth of oral C. albicans cells at day 2. Conclusion: Virgin coconut oil with concentration of 25% was able to inhibit with 2 days time exposure, which is similar with other synthetic antifungals.

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“…Kandungan coconut oil murni dalam sarang lebah madu Trigona spp merupakan kandungan senyawa lain yang merupakan agen antimikrobia, baik bakteri Gram positif maupun Gram negatif (Sia et al, 2010). Cococnut oil juga dapat menghambat pertumbuhan Candida albicans dengan konsentrasi minimum 25% (Ogoblu et al, 2007).…”

Section: S Aureus P Aeruginosa C Albicansunclassified

Daya Antimikrobia Sarang Lebah Madu Trigona spp terhadap Mikrobia Patogen

et al. 2015

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Potensi sarang lebah madu Trigona spp untuk menghasilkan antimikrobia sangat tinggi, tidak hanya pada bagian propolis, melainkan keseluruhan bagian dari sarang yang terdiri dari kantong madu, kantong polen, kantong telur, dan penutup sarang. Sarang lebah madu Trigona spp berfungsi sebagai tempat perlindungan lebah dari predator maupun parasit seperti fungi, jamur dan virus. Sarang lebah madu Trigona spp dapat dijadikan alternatif sumber antimikrobia alami sehingga diperlukan penelitian untuk mendeteksi aktivitas tersebut. Tujuan dari penelitian ini meneliti daya antimikrobia yang dilepaskan sarang lebah madu Trigona spp terhadap pertumbuhan mikrobia dan meneliti komponen kimia utama yang terkandung dalam sarang lebah madu Trigona spp. Metode untuk ekstraksi sarang menggunakan ethanol 70% secara maserasi dan uji antimikrobia yang terdiri dari antibakteri dan antifungi dilakukan dengan metode difusi sumur. Rancangan penelitian yang digunakan dalam bentuk rancangan acak lengkap (RAL). Data yang diperoleh dari hasil pengamatan di laboratorium dianalisis dengan menggunakan statistik parametrik yaitu One way ANOVA. Hasil penelitian menunjukkan aktivitas antimikrobia sarang lebah madu Trigona spp berpengaruh menghambat pertumbuhan mikrobia patogen uji dengan konsentrasi hambat minimal 1% (v/v). Senyawa kimia utama sebagai antimikrobia berasal dari asam lemak dan fenol. Keywords: antimikrobia, sarang lebah, Trigona spp, mikrobia patogen LATAR BELAKANGProduk peternakan Trigona spp yang telah banyak dimanfaatkan adalah madu dan propolis. Propolis merupakan bagian dari sarang lebah madu Trigona spp yang terdiri dari senyawa yang merupakan bahan resin komplek yang dihasilkan oleh lebah madu dari eksudat tanaman (Selvan&Prabhu, 2010). Penelitian sebelumnya tentang propolis atau penutup sarang yang merupakan bagian dari sarang lebah madu Trigona spp membuktikan bahwa propolis memiliki potensi sebagai sumber antimikrobia seperti aktivitas antibakteri mampu menghambat pertumbuhan Campylobacter spp (Fatoni et al., 2008).Berbagai penelitian telah dilakukan untuk mendapatkan antimikrobia alami dari hasil peternakan Trigona spp selain propolis, salah satunya adalah sarang lebah. Sarang lebah merupakan tempat perlindungan bagi koloni lebah dari serangan bakteri, jamur, virus maupun predator, serta sebagai tempat produksi madu, bee pollen, dan tempat tumbuh kembang telur lebah. Kondisi sarang lebah sangat mempengaruhi kualitas madu dan yang dihasilkan. Madu pada umumnya terbebas dari mikrobia patogen. Penurunan kualitas madu dan bahkan tidak layak untuk dikonsumsi disebabkan oleh keberadaan bakteri tertentu dan didominasi oleh Bacillus sp pada sarang lebah (Perez et al., 2013).Potensi sarang lebah madu Trigona spp sebagai sumber antimikrobia sangat menarik untuk diteliti, karena sebagai akibat dari keberadaan antimikrobia tersebut kualitas madu dan kesterilan sarang dapat terjaga. Kandungan senyawa pada sarang lebah madu berfungsi sebagai pelindung dan penentu kualitas madu antara lain flavonoid yang merupakan senyawa fenol a...

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In VitroAntimicrobial Properties of Coconut Oil onCandidaSpecies in Ibadan, Nigeria

Cited by 113 publications

References 17 publications

Preliminary study on the In-Vitro Susceptibility of Mycobacterium tuberculosis Isolates to Virgin Coconut Oil

Preliminary study on the In-Vitro Susceptibility of Mycobacterium tuberculosis Isolates to Virgin Coconut Oil

Exposure time of virgin coconut oil against oral Candida albicans

Daya Antimikrobia Sarang Lebah Madu Trigona spp terhadap Mikrobia Patogen

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