Introduction: Virgin coconut oil (VCO) is an oil which is purely made as a product of the coconut palm and has been used for centuries in the culinary industry and the medical field. Currently, VCO is used widely in medical field due to its effectiveness as antibacterial, antiviral, and antifungal properties. Objective of this experimental study was to prove the effectiveness of virgin coconut oil by determining its exposure time at concentration of 25% to inhibit growth of oral Candida albicans. Method: The research was done by quasi experiment design using the serial dilution method. The exposure time test was performed using a spread plate technique with virgin coconut oil at concentration of 25 % against oral Candida albicans isolated from saliva. Intervals of day 0, 1, 2, and 3 were used. Result: The result showed that virgin coconut oil inhibited the growth of oral C. albicans cells at day 2. Conclusion: Virgin coconut oil with concentration of 25% was able to inhibit with 2 days time exposure, which is similar with other synthetic antifungals.