In vitro antibacterial activity and gas chromatography–mass spectroscopy analysis of Acacia karoo and Ziziphus mauritiana extracts

Priyanka, C.; Kumar, Pradeep; Bankar, Shivakumar P.; Karthik, Loganathan

doi:10.1016/j.jtusci.2014.06.007

Cited by 34 publications

(18 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Its presence as a major phytochemical compound was also in concordance with other studies on chloroformic extract of Acacia karoo root and Artemisia lavandulaefolia essential oil, where Bicyclo[2.2.1]heptan-2-one,1,7,7-trimethyl-, (1S)-was present as a major bioactive phytochemical constituent [56][57].…”

Section: Discussionsupporting

confidence: 90%

Bioprospecting the antimicrobial, antibiofilm and antiproliferative activity of Symplocos racemosa Roxb. Bark phytoconstituents along with their biosafety evaluation and detection of antimicrobial components by GC-MS

Sood

Kumar

Gupta

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The increased occurrence of the antibiotic resistance has added up to the misery of mankind. There is an immediate need to search alternatives and Symplocos racemosa is one such potent medicinal plant . The aim of the study was to explore various activities of its phytoconstituents, confirm their biosafety and identify the active components.Methods: The ethylacetate extract and the major group of phytoconstituents were tested for minimal inhibitory concentration, viable cell count studies, Post antibiotic effect, activity against 34 multi-drug resistant microorganisms, anti-biofilm and anti proliferative potential. The biosafety was evaluated by Ames and MTT assay. In vivo safety of flavonoids was determined by acute oral toxicity test using Swiss albino mice. The antimicrobial components in flavonoids were also identified by using TLC and GC-MS analysis.Results: Ethyl acetate was the best organic extractant. The phytochemical analyses revealed the presence of flavonoids, cardiac glycosides, saponins, tannins, triterpenes and Phytosterols. Flavonoids exhibited the highest antimicrobial potential whereas tannins, triterpenes and phytosterols were completely inactive. Their Minimum Inhibitory Concentration ranged from 0.5-10 mg/ml; exhibited a bactericidal mode of action and a prolonged post antibiotic effect. The phytoconstituents displayed a significant antibiofilm potential by inhibiting the initial cell attachment, disrupting the pre-formed biofilms and reducing the metabolic activity of biofilms. The phytoconstituents were significantly active against the drug-resistant strains of E.coli , MRSA and Salmonella spp. Flavonoids showed significant cytotoxic effect against the RD, L20B and Hep2 cell lines (IC 50 361-494 µg/ml). All the test extracts were biosafe as depicted by the Ames test, MTT assay. Also, flavonoids did not induce any abnormality in biochemical parameters and Organs’ histopathology of the Swiss albino mice during the in vivo acute oral toxicity studies. The flavonoids were resolve into 4 bands (S1-S4) using TLC, where S3 was the most active and its GC-MS analysis revealed the presence of Bicyclo [2.2.1]heptan-2-one,1,7,7-trimethyl-, (1S)- at an RT of 11.14 as the major compound.Conclusions: These findings suggest that Symplocos racemosa bark could be a potential source of antimicrobial metabolites, which could substantially contribute to the arsenal of existing natural drugs for combating the antimicrobial resistance.

show abstract

Section: Discussionsupporting

confidence: 90%

Bioprospecting the antimicrobial, antibiofilm and antiproliferative activity of Symplocos racemosa Roxb. Bark phytoconstituents along with their biosafety evaluation and detection of antimicrobial components by GC-MS

Sood

Kumar

Gupta

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Oxime-, methoxyphenyl, and hexadecanoic acid possess antioxidant and antimicrobial behaviour [51,52]. e compounds cyclotrisiloxane, hexamethyl, and isopropyl myristate have the antioxidant potential [53,54]. Undecane has a broad spectrum of antimicrobial activities [55].…”

Section: Gas Chromatography-mass Spectrometry Analysismentioning

confidence: 99%

Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)

Sikandar

Zhang

Wang

et al. 2020

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

Antioxidants are the radical scavengers that inhibit peroxidation and other free-radical processes, which in return safeguard different organisms from various diseases attributed to radical reactions. Synthetic antioxidants inhibit free radicals, but they also have harmful side effects. However, mycochemicals of natural fungal origin are safe and best substitutes for harmful synthetic chemical antioxidants. The prime objectives of the study include appropriate qualitative and quantitative mycochemical screening, antioxidant potential, and chemical composition of Snef1216 (Penicillium chrysogenum). The study has used aluminium chloride colourimetric method, Folin–Ciocalteu reagent assay, and DPPH (1,1-diphenyl-1-picrylhydrazyl) for analysis of total flavonoid content and phenol content and antioxidant activity, respectively. However, the presence of biologically active compounds was screened through gas chromatography-mass spectrometry (GC-MS). Quantitative analysis demonstrated the existence of flavonoids, glycosides, flavones, saponins, phenols, and catecholic tannins excluding alkaloids, terpenoids, steroids, and gallic tannins. The outcomes exposed total flavonoid content and phenolic content in P. chrysogenum were 85.31 ± 1.23 mg·QE/g and 135.77 ± 1.14 mg·GAE/g, respectively. Snef1216 (P. chrysogenum) displayed the highest free-radical scavenging activity with 63.86% inhibition of DPPH. The analysis confirms that Snef1216 (P. chrysogenum) is an alternative source of natural antioxidants. The obtained data have provided the foundation for its use in agricultural, environmental, and pharmaceutical industries.

show abstract

“…Inhibition of biofilms by ethanolic extract of Chlorella has hence provided an attractive anti-biofilm strategy against P. aeruginosa, which can be resistant to multiple antibiotic agents. The GC MS analysis revealed that Diethyl phthalate at Rt 15.563 (39.28%) and Trimethyl (4-tertbutyl phenoxy) silane at Rt 31.944 (25.63%), as the major compound in the ethanolic extract of Chlorella vulgaris which have been reported to have antibacterial activity 13,14 . The estimation of in-vitro maximal cytotoxic free concentration was done on vero cell line.…”

Section: Discussionmentioning

confidence: 99%

Untitled

2019

IJPSR

View full text Add to dashboard Cite

Background: Biofilm is a layer like aggregates of bacterial cells which are formed on biotic and abiotic surfaces. Pseudomonas aeruginosa plays an essential role in the aggregation of bacteria on biofilm formation. Quorum sensing and extracellular polysaccharide play a vital role in the biofilm formation in P. aeruginosa. The present study was aimed to investigate the anti-biofilm effect of lyophilized extracts of Chlorella. Materials and Methods: The lyophilized aqueous and ethanolic extracts of Chlorella were screened for anti-biofilm activity by MTT assay; microdilution and antibacterial activity were assessed by disc diffusion assay. GC-MS were used to determine the bioactive compound. Results: The lyophilized ethanolic extract of Chlorella showed concentration-dependent inhibition of up to 85% in biofilm, from a concentration of 2 mg/ml to 500 µg/ml by MTT assay. The antibiofilm activity of the significant ethanolic extract of chlorella was ascertained through a scanning electron microscope. The anti-bacterial activity showed a moderate to low inhibition around the disc showing no anti-bacterial activity. GC-MS analyses revealed the presence of 8 major compounds in the ethanolic extract of Chlorella. Cytotoxic results revealed that the ethanolic extract was non-toxic from 1000 µg/ml proving Chlorella extracts to be non-toxic at all concentrations. Conclusion: Current study proves that micro-algae like Chlorella have the potential to inhibit the pathogenic activity of P. aeruginosa in biofilm formation by providing a novel alternative to the conventional anti-microbial agents.

show abstract

In vitro antibacterial activity and gas chromatography–mass spectroscopy analysis of Acacia karoo and Ziziphus mauritiana extracts

Cited by 34 publications

References 10 publications

Bioprospecting the antimicrobial, antibiofilm and antiproliferative activity of Symplocos racemosa Roxb. Bark phytoconstituents along with their biosafety evaluation and detection of antimicrobial components by GC-MS

Bioprospecting the antimicrobial, antibiofilm and antiproliferative activity of Symplocos racemosa Roxb. Bark phytoconstituents along with their biosafety evaluation and detection of antimicrobial components by GC-MS

Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)

Untitled

Contact Info

Product

Resources

About