Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)

Abstract

Antioxidants are the radical scavengers that inhibit peroxidation and other free-radical processes, which in return safeguard different organisms from various diseases attributed to radical reactions. Synthetic antioxidants inhibit free radicals, but they also have harmful side effects. However, mycochemicals of natural fungal origin are safe and best substitutes for harmful synthetic chemical antioxidants. The prime objectives of the study include appropriate qualitative and quantitative mycochemical screening, antioxidant potential, and chemical composition of Snef1216 (Penicillium chrysogenum). The study has used aluminium chloride colourimetric method, Folin–Ciocalteu reagent assay, and DPPH (1,1-diphenyl-1-picrylhydrazyl) for analysis of total flavonoid content and phenol content and antioxidant activity, respectively. However, the presence of biologically active compounds was screened through gas chromatography-mass spectrometry (GC-MS). Quantitative analysis demonstrated the existence of flavonoids, glycosides, flavones, saponins, phenols, and catecholic tannins excluding alkaloids, terpenoids, steroids, and gallic tannins. The outcomes exposed total flavonoid content and phenolic content in P. chrysogenum were 85.31 ± 1.23 mg·QE/g and 135.77 ± 1.14 mg·GAE/g, respectively. Snef1216 (P. chrysogenum) displayed the highest free-radical scavenging activity with 63.86% inhibition of DPPH. The analysis confirms that Snef1216 (P. chrysogenum) is an alternative source of natural antioxidants. The obtained data have provided the foundation for its use in agricultural, environmental, and pharmaceutical industries.

Evaluation of the Antioxidant Activity and Mycochemical Screening of Two <i>Penicillium oxalicum </i>Isolated from Soil and Leaves of <i>Solanum lycopersicum </i>Respectively

“…The best antiradical activity observed with the P. oxalicum extract from the soil, would be due to the phenolic compounds [48]. Indeed, similar results have been reported by other authors who have shown that there is a relationship between the content of phenolic compounds and the antiradical activity of fungi extracts [49,50].…”

Acetylcholine Esterase Inhibitory Effect, Antimicrobial, Antioxidant, Metabolomic Profiling, and an In Silico Study of Non-Polar Extract of The Halotolerant Marine Fungus Penicillium chrysogenum MZ945518

“…Antioxidants play an important role in cell protection by blocking free radicals at their active site and trapping free radicals that cause degenerative processes [ 54 , 55 ]. Previous research has demonstrated that Penicillium chrysogenum has a strong antioxidant capacity [ 11 , 42 ]. The antioxidant activity of P. chrysogenum MZ945518 has an IC 50 of 542.5 µg/mL in the DPPH test.…”

“…There are numerous species within the genus, some of which are commercially important in nutrition, biomedical, and pharmaceutical production [ 10 ]. Due to the abundance of bioactive components, such as flavonoids, alkaloids, minerals, proteins, phenols, tannins, vitamins, and antioxidant characteristics, these organisms were able to biosynthesize a diverse array of primary and secondary metabolites [ 11 ]. Anticancer, antibacterial, and antioxidant effects are demonstrated by many species of this genus [ 12 ].…”

Preliminary Screening for the Anthelmintic Activity of Millettia zechiana Harms and Its Modifying Effect on Albendazole

“…The plant powder, as well as ethanol, ethyl acetate, and petroleum ether extracts, was screened for their phytochemical constituents (alkaloids, saponins, cardiac glycosides, flavonoids, phenols, triterpenoids, and tannins). The phytoconstituents were identified by characteristic colour change using standard procedures by Sikandar et al [ 16 ], performing each experiment in triplicate.…”