In situ Raman study to monitor bioactive glasses reactivity

Abstract

This work, by means of Raman spectroscopy, studies the vibrational modifications of a silica-based sol-gel bioactive glass (77S) in direct contact with a simple TRIS-buffered solution. The novelty of this contribution with respect to similar previous works consists in the adoption of an in situ setup, designed to avoid all kinds of external contamination. The time-dependent formation of the characteristic vibrational features of surface hydroxy-carbonate apatite (HCA) is evidenced, and followed as a function of contact time.

New evaluation of the in vitro response of silicocarnotite monophasic material

“…At higher wavenumber, two very broad bands were observed. The band around 1090 cm À 1 is very characteristic of CO 3 À 2 units in the structure of carboapatite phases [29,31,35,36] although vibrations related to asymmetric stretching modes of Si-O-Si groups has been also reported [37,38] in this region. Moreover, vibrations of -PO 3 and -PO 2 groups in phosphate amorphous phases can be contributing to this broad band [39] between 1000 and 1300 cm À 1 .…”

“…Vibration of ν 2 and ν 4 modes of phosphate groups in apatite phases have been reported around 429-565 and 579-620 cm À 1 respectively. The presence of two broad bands at 570 and 750 cm À 1 can be associated with CO 3 2 À groups in apatite phases [29,31,35,36], and/or with contributions of the combination of vibrations of O-Si-O and O-P-O bonds in amorphous-like phases [37,38].…”

Surface characterization of silicate bioceramics

“…Bonino et al used Raman spectroscopy to analyse the transformations of a sol-gel bioactive glass immersed in tris buffer, thus avoiding issues of contamination that otherwise arise when the samples are removed from a solution and dried [62]. Jell et al [63] used Raman microscopy to detect the activity of foetal osteoblasts (FOBs) in contact with media containing the dissolution products of BG particles.…”

Morphological and Structural Study of a Novel Porous Nurse’s A Ceramic with Osteoconductive Properties for Tissue Engineering

“…Bands of around 440, 540, 590, and 700 cm −1 have been assigned in phosphate that contained silicate glasses in relation to the vibrations in phosphorus-oxygen units [24,25]. Bands of around 695 and 780 cm −1 have been reported in the Raman spectrum of calcium–phosphate glasses [24,25].…”