<i>Debaryomyces hansenii</i>growth in nonsterile seawater ClO<sub>2</sub>- peptone-containing medium

Ramírez-Orozco, Martín; Hernández-Saavedra, Norma Y.; Ochoa, J.L.

doi:10.1139/w01-056

Cited by 13 publications

(3 citation statements)

References 23 publications

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“…Another very interesting observation was that D. hansenii has high tolerance to chlorine dioxide (ClO 2 ), a powerful biocide152. This resistance could be used to cultivate the yeast in non‐sterile media containing up to 0.3 mg/l ClO 2 to control unwanted microorganisms.…”

Section: Phylogeny Ecology Physiology and Molecular Biologymentioning

confidence: 99%

Debaryomyces hansenii — an extremophilic yeast with biotechnological potential

Breuer

Harms

2006

Yeast

268

175

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We illuminate the ecological, physiological and genetic characteristics of the yeast Debaryomyces hansenii in the view of our belief that this metabolically versatile, non-pathogenic, osmotolerant and oleaginous microorganism represents an attractive target for fundamental and applied biotechnological research. To this end, we give a broad overview of extant biotechnological procedures using D. hansenii, e.g. in the manufacture of various foods, and propose research into the heterologous synthesis of a range of fine chemicals.

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Section: Phylogeny Ecology Physiology and Molecular Biologymentioning

confidence: 99%

Debaryomyces hansenii — an extremophilic yeast with biotechnological potential

Breuer

Harms

2006

Yeast

268

175

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“…In this regard, microbial cell factories that can convert various carbon sources found in industrial waste and side-streams into valuable products are highly sought after as key enablers of sustainable and circular production processes. Whereas the industrial workhorse Saccharomyces cerevisiae can only ferment a subset of monosaccharides, several so called nonconventional yeast species display broad substrate ranges, often including various monosaccharides as well as disaccharides and sometimes even more complex oligo- and polysaccharides (Ramírez-Orozco et al 2001 , Lobs et al 2017 , Baptista and Domingues 2022 , Šuchová et al 2022 ). To develop a nonconventional yeast into a cell factory, detailed metabolic characterization on a molecular level remains an essential prerequisite.…”

Section: Introductionmentioning

confidence: 99%

Split-marker-mediated genome editing improves homologous recombination frequency in the CTG clade yeastCandida intermedia

Peri

Faria-Oliveira

Larsson

et al. 2023

FEMS Yeast Research

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Genome editing toolboxes are essential for the exploration and exploitation of non-conventional yeast species as cell factories, as they facilitate both genome studies and metabolic engineering. The non-conventional yeast Candida intermedia is a biotechnologically interesting species due to its capacity to convert a wide range of carbon sources, including xylose and lactose found in forestry and dairy industry waste and side-streams, into added-value products. However, possibilities of genetic manipulation have so far been limited due to lack of molecular tools for this species. We describe here the development of a genome editing method for C. intermedia, based on electroporation and gene deletion cassettes containing the Candida albicans NAT1 dominant selection marker flanked by 1,000 base pair sequences homologous to the target loci. Linear deletion cassettes targeting the ADE2 gene originally resulted in <1% targeting efficiencies, suggesting that C. intermedia mainly uses non-homologous end joining for integration of foreign DNA fragments. By developing a split-marker based deletion technique for C. intermedia, we successfully improved the homologous recombination rates, achieving targeting efficiencies up to 70%. For marker-less deletions, we also employed the split-marker cassette in combination with a recombinase system, which enabled the construction of double deletion mutants via marker recycling. Overall, the split marker technique proved to be a quick and reliable method for generating gene deletions in C. intermedia, which opens the possibility to uncover and enhance its cell factory potential.

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“…Though its strong effect against bacteria, it has been shown to have a degree of fungicidal activity in solution (12), especially against some mold growth (19). Authors (13) have found that the marine yeast Debaryomyces hansenii is highly tolerant to chlorine dioxide, in such a way that helped to avoid the growth of unwanted microorganisms while allowing the yeast development. Disinfectants containing chloride and persulphate were ineffective in destroying yeast cells both in suspensions and in biofilm formations (16).…”

Section: Introductionmentioning

confidence: 99%

Chlorine dioxide against bacteria and yeasts from the alcoholic fermentation

Meneghin¹,

Reis²,

Almeida³

et al. 2008

Braz. J. Microbiol.

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The ethanol production in Brazil is carried out by fed-batch or continuous process with cell recycle, in such way that bacterial contaminants are also recycled and may be troublesome due to the substrate competition. Addition of sulphuric acid when inoculum cells are washed can control the bacterial growth or alternatively biocides are used. This work aimed to verify the effect of chlorine dioxide, a well-known biocide for bacterial decontamination of water and equipments, against contaminant bacteria (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides) from alcoholic fermentation, through the method of minimum inhibitory concentration (MIC), as well as its effect on the industrial yeast inoculum. Lower MIC was found for B. subtilis (10 ppm) and Leuconostoc mesenteroides (50 ppm) than for Lactobacillus fermentum (75 ppm) and Lactobacillus plantarum (125 ppm). Additionally, these concentrations of chlorine dioxide had similar effects on bacteria as 3 ppm of Kamoran® (recommended dosage for fermentation tanks), exception for B. subtilis, which could not be controlled at this Kamoran® dosage. The growth of industrial yeasts was affected when the concentration of chlorine dioxide was higher than 50 ppm, but the effect was slightly dependent on the type of yeast strain. Smooth yeast colonies (dispersed cells) seemed to be more sensitive than wrinkled yeast colonies (clustered cells/pseudohyphal growth), both isolated from an alcoholproducing unit during the 2006/2007 sugar cane harvest. The main advantage in the usage of chlorine dioxide that it can replace antibiotics, avoiding the selection of resistant populations of microorganisms.

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Debaryomyces hanseniigrowth in nonsterile seawater ClO₂- peptone-containing medium

Cited by 13 publications

References 23 publications

Debaryomyces hansenii — an extremophilic yeast with biotechnological potential

Debaryomyces hansenii — an extremophilic yeast with biotechnological potential

Split-marker-mediated genome editing improves homologous recombination frequency in the CTG clade yeastCandida intermedia

Chlorine dioxide against bacteria and yeasts from the alcoholic fermentation

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Debaryomyces hanseniigrowth in nonsterile seawater ClO2- peptone-containing medium

Cited by 13 publications

References 23 publications

Debaryomyces hansenii — an extremophilic yeast with biotechnological potential

Debaryomyces hansenii — an extremophilic yeast with biotechnological potential

Split-marker-mediated genome editing improves homologous recombination frequency in the CTG clade yeastCandida intermedia

Chlorine dioxide against bacteria and yeasts from the alcoholic fermentation

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Product

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Debaryomyces hanseniigrowth in nonsterile seawater ClO₂- peptone-containing medium