Clinacanthus nutansExtracts Are Antioxidant with Antiproliferative Effect on Cultured Human Cancer Cell Lines

Abstract

Clinacanthus nutans Lindau leaves (CN) have been used in traditional medicine but the therapeutic potential has not been explored for cancer prevention and treatment. Current study aimed to evaluate the antioxidant and antiproliferative effects of CN, extracted in chloroform, methanol, and water, on cancer cell lines. Antioxidant properties of CN were evaluated using DPPH, galvinoxyl, nitric oxide, and hydrogen peroxide based radical scavenging assays, whereas the tumoricidal effect was tested on HepG2, IMR32, NCL-H23, SNU-1, Hela, LS-174T, K562, Raji, and IMR32 cancer cells using MTT assay. Our data showed that CN in chloroform extract was a good antioxidant against DPPH and galvinoxyl radicals, but less effective in negating nitric oxide and hydrogen peroxide radicals. Chloroform extract exerted the highest antiproliferative effect on K-562 (91.28 ± 0.03%) and Raji cell lines (88.97 ± 1.07%) at 100 μg/ml and the other five cancer cell lines in a concentration-dependent manner, but not on IMR-32 cells. Fourteen known compounds were identified in chloroform extract, which was analysed by gas chromatography—mass spectra analysis. In conclusion, CN extracts possess antioxidant and antiproliferative properties against cultured cancer cell lines, suggesting an alternate adjunctive regimen for cancer prevention or treatment.

Phytochemical Evaluation of Polyherbal Formulation of Clinacanthus nutans and Elephantopus scaber to Identify Flavonoids

“…11,12 The literature review suggest that Clinacanthus nutans and Elephantopus scaber has antioxidant properties, which may be responsible and constructive for faster wound healing. [13][14][15] Further investigation on new poly herbal formulation in the treatment of wound and isolation of bioactive compounds is currently in progress.…”

Evaluation on the effects of P. ostreatus spent mushroom compost and BAP hormone towards C. nutans in vitro culture

“…This is due to medicinal plant implies it important as a therapeutic agent and more [1]. One of the medicinal plant which can give benefits in medicine line through proven research is Clinacanthus nutans, as it can treat several diseases including cancer [2] without giving signs of toxicity and morbidity [3]. Other than that, this plants also were generally used in Indonesia and Thailand for treating skin rashes, insects and snake bites, diabetes mellitus, fever, diuretics and dysuria [4].…”

Changes in phytochemical contents in different parts of Clinacanthus nutans (Burm. f.) lindau due to storage duration

“…Besides, it also possesses anti-hepatitis, anti-herpes, and anti-inflammatory properties. Furthermore, this herb is also used to prevent and treat cancer (Sookmai et al, 2011;Wanikiat et al, 2008;Yong et al, 2013).…”