Evaluation on the effects of P. ostreatus spent mushroom compost and BAP hormone towards C. nutans in vitro culture

Hashim, Saiyidah Nafisah; Anuar, Wan Nurul Hidayah Wan; Tay, Chia Chay; Mahmud, Siti Hamidah Radiyah Shiekh

doi:10.4314/jfas.v9i4s.55

Cited by 2 publications

(2 citation statements)

References 15 publications

(18 reference statements)

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“…Both powdered and extracts SMC had emerged positive feedback on the production of shoots with varieties of length. There is similar finding as recoded for Hashim et al (2017). However, the extracellular enzymes in the extracts exerted an effect by elongating the cell wall of the plants and provide a rapid secondary growth (Hayashi et al, 2005).…”

supporting

confidence: 77%

Enhancement Effect of BAP and Spent Mushroom Compost in Micro-Propagation of Sabah Snake Grass

Anuar¹,

Tan²,

Mahmud³

et al. 2019

American Journal of Biochemistry and Biotechnology

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Medicinal plants which is Clinacantus nutans (C. nutans) that known as Sabah Snake Grass or Belalai Gajah can treat several diseases including cancer. In order to provide and grow this plant in large number for health and pharmaceutical industry, one of the solutions is through the plant tissue culture technique. In this experiment, C. nutans was cultured under controlled conditions which to provide sterile plantlets. The surface sterilization using three methods which involved ethanol, Clorox and mercuric chloride. In the comparison of them, it was found that the use of 70% ethanol (5 min) followed by 30% clorox (5 min) then with 0.1% mercuric chloride for one hour was the most effective sterilization method which gave the highest survival rate (100%) and zero contamination rate. The sterile explants were then cultured in media containing different concentrations of Benzylaminopurine (BAP) and Pleurotus ostreatus Spent Mushroom Compost (SMC). The SMC was tested and compared in powder and extracted form. The observations were made every week on responds of the culture using variables which are number of shoots, length of shoots and number of leaves. This article was aimed to evaluate the enhancement effect of BAP and SMC in micro-propagation of Sabah Snake Grass. The authors found that the best concentration for BAP is 1.0 µM. It produced 3.5±0.926 number of shoots and 2 µM BAP produced 7.286±1.496 number of leaves in their findings. When BAP and SMC were tested in combination, they managed to produce high number of shoots and leaves. Their result revealed a number of shoots (5.750±0.500) produced by 2% extract of SMC and some leaves (11.890±1.965) by 8% extract of SMC when both were cultured together with 1.0 µM BAP respectively. The authors thought that there was enhancement effect on the combination of BAP with extracts of SMC which gave about double regeneration rate as compared with the BAP alone and this method had high potential for future research in producing mass propagation of C. nutan and other related study by reducing the usage of plant growth regulator which may reduce the cost of micro-propagation of tissue culture plants.

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supporting

confidence: 77%

Enhancement Effect of BAP and Spent Mushroom Compost in Micro-Propagation of Sabah Snake Grass

Anuar¹,

Tan²,

Mahmud³

et al. 2019

American Journal of Biochemistry and Biotechnology

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“…It is widely cultivated in tropical Asia and has long been utilized as a medicinal plant in China, Thailand, and Malaysia [5,6]. The plant has long been used in the folk medicine due to the potential health promoting and pharmacological attributes [7], which are mainly ascribed to the presence of antioxidant constituents such as phenolic acids and flavonoids [8,9,10] According to Intan Suraya et al [11], an ethnobotanical survey in Singaporean communities revealed that C. nutans fresh herbs are consumed as a traditional medicine for its general detoxification purposes. The antioxidant activity is explained due to the presence of phenolic chemicals such as flavonoids, anthocyanins, and others [12].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Compounds and Antioxidants Analysis of <i>Clinacanthus nutans </i>Leaf and Stem Extracts

Hasyim,

Sidik,

Chay

et al. 2023

Advances in Science and Technology

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Clinacanthus nutans consists with multiple medicinal properties. The plant is also known to be a source of biologically active compounds, particularly those with an antioxidant capacity. The phytochemical studies of C. nutans plant extract was mostly reported from its leaf part. Thus, the objectives of this study are to assess the phytochemical constituents and antioxidant activity of C. nutans leaf and stem. Methanol was used as solvent for the green extraction. Next, phytochemical screening, Fourier transform infrared (FTIR) analysis, total phenolic (TPC) and flavonoids contents (TFC), quercetin flavonoid compound analysis and in vitro antioxidant activity were performed on C. nutans leaf and stem. In comparison to stem extract, the leaf extract showed a more favorable appearance of metabolites. In FTIR analysis, both sample extracts revealed the presence of hydroxyl and carbonyl functional groups, which are important in antioxidant action. The TPC (228.06 mg GAE/g DW) and TFC (29.76 mg QE/g DW) values were higher in the leaf extract than the stem extract. Quercetin quantification in HPLC analysis revealed that the leaf extract (21.33 g/g) contained more quercetin than the stem extract (18.88 g/g). Between the extracts, leaf providing more antioxidant values of DPPH (15.50%) and FRAP (15.68 gFeSO4/g) than stem. The greatest association was identified between TFC and FRAP reducing activity (r = 0.977, p<0.05) indicating that the flavonoid present in the extract contributed to C. nutans' antioxidant capacity.

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Evaluation on the effects of P. ostreatus spent mushroom compost and BAP hormone towards C. nutans in vitro culture

Abstract: Realization in minimizing production cost for in vitro culture had brought to a study on application of P. ostreatus spent mushroom compost

Cited by 2 publications

References 15 publications

Enhancement Effect of BAP and Spent Mushroom Compost in Micro-Propagation of Sabah Snake Grass

Enhancement Effect of BAP and Spent Mushroom Compost in Micro-Propagation of Sabah Snake Grass

Phytochemical Compounds and Antioxidants Analysis of <i>Clinacanthus nutans </i>Leaf and Stem Extracts

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