Hydroxyapatite particles maintain peri-implant bone mantle during osseointegration in osteoporotic bone

Tami, Andrea; Leitner, Melanie M.; Baucke, Michelle; Mueller, Timothy I.; Lenthe, G. Harry van; Müller, Ralph; Ito, Keita

doi:10.1016/j.bone.2009.07.090

Cited by 25 publications

(15 citation statements)

References 39 publications

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“…28,29 From the preliminary short-term in vivo study, HA-coated Ti implants showed excellent bone-forming ability. The new bone was formed on HA-coated Ti implants along the implant surface to the apex of the implant.…”

Section: Discussionmentioning

confidence: 98%

Improved biocompatibility of hydroxyapatite thin film prepared by aerosol deposition

Park

Kim

et al. 2010

J Biomed Mater Res

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Technical development for an efficient coating of bioactive materials improves the characteristics of a fully functional implant. The aim of this study was to investigate the osteoinductive effect of a newly developed hydroxyapatite (HA)-coating technique using aerosol deposition without post-heat treatment [room temperature (RT) group] on the titanium (Ti) dental implant in vitro and in vivo, compared with that of HA coating with post-heat treatment (HT-400 group) or machined surface (control group). Cell proliferation or attachment on the HA-coated Ti surface was assessed using tetrazolium salt, WST-8 or scanning electron microscopy (SEM). Human osteoblasts (HOB) on RT group were well attached and grew alike in the control or HT-400 group. The alkaline phosphatase activity of HOB cultured on RT and HT-400 group was significantly higher than the control group (p < 0.05). Evaluation by SEM, TEM, and XRD demonstrated that aerosol deposition facilitated HA particles to form a dense and uniform HA layer in the RT group despite no post-heating. In a rabbit tibia model (n = 3), the ratios of bone implant contact and bone area in the RT group (49.88%, 86.05%) were greater than in the HT-400 group (38.82%, 77.34%) or the control (28.31%, 73.86%). The finding of this study showed that the HA coating using aerosol deposition without post-heat treatment has a good biocompatibility, and provide a promoting strategy to enhance osseointegration in the application of the dental implant.

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Section: Discussionmentioning

confidence: 98%

Improved biocompatibility of hydroxyapatite thin film prepared by aerosol deposition

Park

Kim

et al. 2010

J Biomed Mater Res

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“…the OV/TV measurement) can predict mechanical fixation of the implant. Other studies have examined the correlation between mechanical fixation and μCT measured BV/TV in ROIs within 200 μm (Tami et al ., 2009) and 500 μm (Schouten et al ., 2009) of the implant interface, and reported poor correlations. It is also important to realize that the mechanical fixation of the implant is not only related to the bone close to the implant interface and in direct contact with the implant, but also is critically dependent on structural characteristics of peri‐implant bone trabeculae in a relatively broad zone around the implant (Gabet et al ., 2010).…”

Section: Discussionmentioning

confidence: 99%

Limitations of using micro‐computed tomography to predict bone–implant contact and mechanical fixation

Liu

Broucek

Virdi

et al. 2011

Journal of Microscopy

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Fixation of metallic implants to bone through osseointegration is important in orthopedics and dentistry. Model systems for studying this phenomenon would benefit from a non-destructive imaging modality so that mechanical and morphological endpoints can more readily be examined in the same specimens. The purpose of this study was to assess the utility of an automated micro computed tomography (μCT) program for predicting bone-implant contact (BIC) and mechanical fixation strength in a rat model. Femurs in which 1.5 mm diameter titanium implants had been in place for four weeks were either embedded in polymethylmethacrylate (PMMA) for preparation of 1 mm thick cross-sectional slabs (16 femurs: 32 slabs) or were used for mechanical implant pull-out testing (n = 18 femurs). All samples were scanned by μCT at 70 kVp with 16 μm voxels and assessed by the manufacturer's software for assessing “osseointegration volume per total volume” (OV/TV). OV/TV measures bone volume per total volume (BV/TV) in a 3-voxel thick ring that by default excludes the 3 voxels immediately adjacent to the implant in order to avoid metal-induced artifacts. The plastic-embedded samples were also analyzed by backscatter scanning electron microscopy (bSEM) to provide a direct comparison of OV/TV with a well-accepted technique for BIC. In μCT images in which the implant was directly embedded within PMMA, there was a zone of elevated attenuation (> 50% of the attenuation value used to segment bone from marrow) which extended 48 μm away from the implant surface. Comparison of the bSEM and μCT images showed high correlations for BV/TV measurements in areas not affected by metal-induced artifacts. In addition for bSEM images, we found that there were high correlations between peri-implant BV/TV within 12 μm of the implant surface and BIC (correlation coefficients ≥ 0.8, p < 0.05). OV/TV as measured on μCT images was not significantly correlated with BIC as measured on the corresponding bSEM images. However, OV/TV was significantly, but weakly, correlated with implant pull-out strength (r=0.401, p=0.049) and energy to failure (r=0.435, p=0.035). Thus, the need for the 48 μm thick exclusion zone in the OV/TV program to avoid metal-induced artifacts with the scanner used in this study means that it is not possible to make bone measurements sufficiently close to the implant surface to obtain an accurate assessment of BIC. Current generation laboratory-based μCT scanners typically have voxel sizes of 6–8 μm or larger which will still not overcome this limitation. Thus, peri-implant bone measurements at these resolutions should only be used as a guide to predict implant fixation and should not be over-interpreted as a measurement of BIC. Newer generation laboratory-based μCT scanners have several improvements including better spatial resolution and x-ray sources and appear to have less severe metal-induced artifacts, but will need appropriate validation as they become available to researchers. Regardless of the μCT scanner being used, we recommend that de...

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“…Reconstructions of mandibular bone defects using HAp have been in clinical practice for more than 20 years now [10]. Fillers based on HAp have also been used for the reconstruction of osteoporotic bone defects prior to fixation [11]. HAp in combination with bisphosphonates has also showed satisfactory results in the treatment and reconstruction of osteoporotic bone [12].…”

Section: Introductionmentioning

confidence: 99%

Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones

Ignjatović

Ajduković

Savić

et al. 2012

J Mater Sci: Mater Med

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Indications exist that paramagnetic calcium phosphates may be able to promote regeneration of bone faster than their regular, diamagnetic counterparts. In this study, analyzed was the influence of paramagnetic cobalt-substituted hydroxyapatite nanoparticles on osteoporotic alveolar bone regeneration in rats. Simultaneously, biocompatibility of the material was tested in vitro, on osteoblastic MC3T3-E1 and epithelial Caco-2 cells in culture. The material was shown to be biocompatible and nontoxic when added to epithelial monolayers in vitro, while it caused a substantial decrease in the cell viability as well as deformation of the cytoskeleton and cell morphology when incubated with the osteoblastic cells. In the course of six months after the implantation of the material containing different amounts of cobalt, ranging from 5 – 12 wt%, in the osteoporotic alveolar bone of the lower jaw, the following parameters were investigated: histopathological parameters, alkaline phosphatase and alveolar bone density. The best result in terms of osteoporotic bone tissue regeneration was observed for hydroxyapatite nanoparticles with the largest content of cobalt ions. The histological analysis showed a high level of reparatory ability of the nanoparticulate material implanted in the bone defect, paralleled by a corresponding increase in the alveolar bone density. The combined effect of growth factors from autologous plasma admixed to cobalt-substituted hydroxyapatite was furthermore shown to have a crucial effect on the augmented osteoporotic bone regeneration upon the implantation of the biomaterial investigated in this study.

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Hydroxyapatite particles maintain peri-implant bone mantle during osseointegration in osteoporotic bone

Cited by 25 publications

References 39 publications

Improved biocompatibility of hydroxyapatite thin film prepared by aerosol deposition

Improved biocompatibility of hydroxyapatite thin film prepared by aerosol deposition

Limitations of using micro‐computed tomography to predict bone–implant contact and mechanical fixation

Nanoparticles of cobalt-substituted hydroxyapatite in regeneration of mandibular osteoporotic bones

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