Improved biocompatibility of hydroxyapatite thin film prepared by aerosol deposition

Abstract

Technical development for an efficient coating of bioactive materials improves the characteristics of a fully functional implant. The aim of this study was to investigate the osteoinductive effect of a newly developed hydroxyapatite (HA)-coating technique using aerosol deposition without post-heat treatment [room temperature (RT) group] on the titanium (Ti) dental implant in vitro and in vivo, compared with that of HA coating with post-heat treatment (HT-400 group) or machined surface (control group). Cell proliferation or attachment on the HA-coated Ti surface was assessed using tetrazolium salt, WST-8 or scanning electron microscopy (SEM). Human osteoblasts (HOB) on RT group were well attached and grew alike in the control or HT-400 group. The alkaline phosphatase activity of HOB cultured on RT and HT-400 group was significantly higher than the control group (p < 0.05). Evaluation by SEM, TEM, and XRD demonstrated that aerosol deposition facilitated HA particles to form a dense and uniform HA layer in the RT group despite no post-heating. In a rabbit tibia model (n = 3), the ratios of bone implant contact and bone area in the RT group (49.88%, 86.05%) were greater than in the HT-400 group (38.82%, 77.34%) or the control (28.31%, 73.86%). The finding of this study showed that the HA coating using aerosol deposition without post-heat treatment has a good biocompatibility, and provide a promoting strategy to enhance osseointegration in the application of the dental implant.

Biomechanical evaluation and surface characterization of a nano-modified surface on PEEK implants: a study in the rabbit tibia

“…15,16 HA has been found to be an excellent coating material for enhanced osseointegration and previous studies have shown a significantly increased rate of bone formation compared to untreated surfaces. [17][18][19][20] The modification converts the PEEK from bioinert to bioactive, since the synthesized HA bound to the implant surface blends into the natural HA in the bone. 21 A commonly applied surface-coating method is the plasma spray technique.…”

Influence of thin carbonate-containing apatite coating with molecular precursor method to zirconia on osteoblast-like cell response

“…Accordingly, cell proliferation on TZP-CA may also be mediated by Ca in addition to increased surface hydrophilicity as previously reported 31) . Osteoblasts cultured on HA-coated Ti showed high differentiation and mineralization compared with non-coated Ti 32,33) . Expression of ALP and parathyroid hormone response were higher in cultures grown on HA than in cultures grown on Ti 34) , and the in vitro formation of extracellular matrices was greater on CaP coatings than on Ti.…”

Dense and highly textured coatings obtained by aerosol deposition method from Ti3SiC2 powder: Comparison to a dense material sintered by Spark Plasma Sintering

“…4 Many kinds of materials have already been deposited by ADM, including non-oxides, [30][31][32][33][34] simple ceramic oxides, [35][36][37][38][39] apatites, 40 ferrites, 41 perovskites, 35,[42][43][44][45][46][47] spinel, 48 and more recently some composite materials with ceramic-ceramic, 49,50 ceramic-metal 51 or ceramic-organic 52,53 combinations. These coatings have many application possibilities in different fields such as batteries, 54 thermal barriers, electrolytes or anticorrosion coatings for SOFC, 55 catalysis, 36,56 biomedicine, 36 membranes, 57 microdevices 35 and many others.…”