Influence of thin carbonate-containing apatite coating with molecular precursor method to zirconia on osteoblast-like cell response

Abstract

The influence of a thin carbonate-containing hydroxyapatite (CA) coating to tetragonal zirconia polycrystal (TZP) on osteoblastlike cell response was investigated. TZP disks were subjected to blasting and acid etching. Thin CA coatings were deposited by the molecular precursor method (TZP-CA). Initial cell adhesion of mouse osteoblast-like cells MC3T3-E1 was enhanced, and marked progress of actin filaments was observed on TZP-CA compared to on TZP. After 3, 5 or 7 days, cell proliferation on TZP-CA was significantly higher than that on TZP. Alkaline phosphatase activity was slightly lower on TZP-CA than on TZP at 7 days, and no difference was observed at 14 or 21 days. At 28 days incubation, collagenous fibers with mineral precipitants accompanied by phosphorous and amino groups were observed. These results indicate that thin CA coating with molecular precursor method offers promise as a means of enhancing cell response, particularly initial adhesion and proliferation of MC3T3-E1 cells.

Calcium orthophosphate deposits: Preparation, properties and biomedical applications

“…Later, the in vivo experiments showed a significantly higher percentage of bone contact of the coated implants if compared with the uncoated ones [314,316,317]. Positive results were also obtained with cells [318].…”

Evaluation of highly carbonated hydroxyapatite bioceramic implant coatings with hierarchical micro-/nanorod topography optimized for osseointegration

“…7,8 According to the substitution of carbonate groups for hydroxyl groups or phosphate ions, carbonated apatite can be categorized into A-type or the more diffused B-type, and the latter has been verified to cause a decrease in crystallinity and hence an increase in solubility. 9 Thus far, carbonated hydroxyapatite (CHA) has mostly been applied as ceramic bone substitutes; [10][11][12] meanwhile, it has also gradually become employed as coatings on implant substrates 13,14 and drug delivery systems. 15 CHA-related investigations have largely reached a consensus on its excellent biocompatibility and bio-resorption properties, yet conflicting results regarding the biological effects on osteoblasts, including adhesion, proliferation and osteogenic differentiation, are still being reported.…”

“…15 CHA-related investigations have largely reached a consensus on its excellent biocompatibility and bio-resorption properties, yet conflicting results regarding the biological effects on osteoblasts, including adhesion, proliferation and osteogenic differentiation, are still being reported. [11][12][13]15,16 The genuine biological functions of CHA biomaterials compared with those of HA products still remain ambiguous and relatively undesirable, mostly because the content of carbonate incorporated into HA was either undetermined or limited to a low concentration range according to the available literature. As a matter of fact, HA with a higher carbonate content mimicking early biomineralization could contribute to better cell behaviors, as implied by a study involving a wide spectrum of carbonate concentrations from 0 to 16.1 wt%.…”

Ca-P spots modified zirconia by liquid precursor infiltration and the effect on osteoblast-like cell responses

“…Ca-P spots formation would avoid the crack occurring for its cluster-structure. Researchers focus on the fabrication of bioactive coatings on zirconia surface [19][20][21] , however, the coatings could not combine with substrates well due to their different physicochemical properties, such as mismatched thermal expansion coefficients 28) . In this study, Ca-P spots anchored with the zirconia (Figs.…”

“…There are many studies regarding zirconia surface modifications to enhance osseointegration, involving machining 13) , sandblasting 14,15) , laser treatment 16) , plasma spraying 17) , sol-gel 18) and surface bioactive coatings [19][20][21] . However, surface modification of zirconia ceramics is technically difficult 22) due to its high hardness.…”