Hydrophilic interaction liquid chromatography/electrospray mass spectrometry determination of acyclovir in pregnant rat plasma and tissues

Abstract

Reversed-phase chromatography is the most common means of separation for small drug molecules. However, polar drugs may suffer from poor retention and peak shape in reversed-phase high-performance liquid chromatography (RP-HPLC). Hydrophilic interaction liquid chromatography (HILIC) provides a viable alternative to RP-HPLC and is an excellent way to separate polar compounds. This paper describes a HILIC/ESI-MS/MS assay for the determination of acyclovir from rat plasma, amniotic fluid, placental tissue, and fetal tissue. The isocratic separation utilizes an underivatized silica column with an acetonitrile/formate buffer mobile phase (80:20). The method is validated over a range of 50 ng/mL to 50 micro g/mL with % error and % relative standard deviation of <15% over 3 days. All samples are prepared by acetonitrile protein precipitation, which yields high recovery (>84% for acyclovir). This assay can be applied to the pharmacokinetic study of the placental transfer of acyclovir.

Determination of Rebaudioside A in Mixed beer, Beer-based Beverages and Lemonades

“…HILIC technika nalezla nejprve uplatnění při separacích sacharidů, aminokyselin a peptidů (Strege, 1998), v posledních letech ale rychle roste počet nově vyvinutých nových kolon pro HILIC a aplikací pro separace v oblasti analýz životního prostředí, potravin, přírodních látek i syntetických léčiv, iontových i neiontových tenzidů. Významnou výhodou HILIC separací je zvýšená citlivost analýz pomocí kapalinové chromatografi e s hmotnostní detekcí díky zlepšené ionizaci elektrosprejem (ESI) v mobilních fázích s vysokým obsahem acetonitrilu (Brown et al, 2002;Jandera, 2011).…”

“…However, the number of newly developed columns for HILIC and applications for separations in the area of environment, foods, natural compounds, synthetic drugs and nonionic surfactants has grown very quickly in the last years. An important benefi t of HILIC separations is higher sensitivity of LC-MS analyses because of improved electrospray ionization (ESI) in mobile phases with high content of acetonitrile (Brown at al., 2002;Jandera, 2011).…”

Critical Review of Development, Validation, and Transfer for High Throughput Bioanalytical LC-MS/MS Methods

“…HILIC also uses water-miscible polar organic solvents such as acetonitrile instead of water-immiscible solvents like hexane and chloroform. LC-MS/MS using HILIC on silica column has been extensively studied and reviewed [68][69][70][71][72][73][74]. Because of the higher organic content in the mobile phase and the more favorable solution chemistry for ionization (acidic mobile phase for basic compounds and neutral mobile phase for acidic compounds), sensitivity improvement was observed [75].…”

A review of analytical methods for the determination of trichloroethylene and its major metabolites chloral hydrate, trichloroacetic acid and dichloroacetic acid

“…This avoids potential issues with TCA being converted to DCA upon deravatization. In addition, the usefulness of both HILIC and HILIC-ion exchange with several small polar compounds, including DCA, has been illustrated (Olsen, 2001;Brown et al, 2002;Grumbach et al, 2003;Schlichtherle-Cerny et al, 2003;Strege et al, 2000;Naidong, 2003;Dixon et al, 2004). Owing to the suitability of HILIC for HAA analysis, more studies with DCA and TCA using HILIC and/or HILIC-ion exchange chromatography are likely to be published.…”