Hydrolytic Enzymes for Chemical Warfare Agent Decontamination

Blum, Marc‐Michael; Richardt, André

doi:10.1002/9783527621620.ch9

Cited by 4 publications

(1 citation statement)

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“…There are many research papers and patents concerning the decontamination of nerve agents that detail various formulations of solutions, microemulsions, bled compositions, fibers, and enzymes. − Decontamination is defined as the process of removing or neutralizing chemical agents from people, equipment, and the environment and can be grouped into two main categories: (i) physical and (ii) chemical removal of the contaminant. In this section, we briefly describe several decontamination systems and applied techniques.…”

Section: Decontaminationmentioning

confidence: 99%

Destruction and Detection of Chemical Warfare Agents

et al. 2011

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Section: Decontaminationmentioning

confidence: 99%

Destruction and Detection of Chemical Warfare Agents

et al. 2011

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Haloalkane dehalogenases: Biotechnological applications

Koudeláková

Bidmanová

Dvořák

et al. 2012

Biotechnology Journal

137

120

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Haloalkane dehalogenases (EC 3.8.1.5, HLDs) are α/β-hydrolases which act to cleave carbon-halogen bonds. Due to their unique catalytic mechanism, broad substrate specificity and high robustness, the members of this enzyme family have been employed in several practical applications: (i) biocatalytic preparation of optically pure building-blocks for organic synthesis; (ii) recycling of by-products from chemical processes; (iii) bioremediation of toxic environmental pollutants; (iv) decontamination of warfare agents; (v) biosensing of environmental pollutants; and (vi) protein tagging for cell imaging and protein analysis. This review discusses the application of HLDs in the context of the biochemical properties of individual enzymes. Further extension of HLD uses within the field of biotechnology will require currently limiting factors - such as low expression, product inhibition, insufficient enzyme selectivity, low affinity and catalytic efficiency towards selected substrates, and instability in the presence of organic co-solvents - to be overcome. We propose that strategies based on protein engineering and isolation of novel HLDs from extremophilic microorganisms may offer solutions.

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