Human platelet lysate stimulates high-passage and senescent human multipotent mesenchymal stromal cell growth and rejuvenation in vitro

Griffiths, Sarah; Baraniak, Priya R.; Copland, Ian B.; Nerem, Robert M.; McDevitt, Todd C.

doi:10.1016/j.jcyt.2013.05.020

Cited by 80 publications

(79 citation statements)

References 50 publications

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“…In Schallmoser et al study, pooled platelet concentrates were subjected to one freeze-thaw cycle at - (3200 cells/ well) at the first day of seeding, and started to reach stationary phase at day-9 of culture. Their study results [28][29][30] were in agreement with our results.…”

Section: Discussionsupporting

confidence: 92%

“…39 Outdated or fresh platelet lysates were capable to promote proliferation of human adiposederived stem cells, while maintaining hADSC phenotype (surface markers) despite of the variations in platelet lysate preparation. 10,20,28,29,33 The limitation in this study was the fact that CD73 conjugated APC was not measured in all samples (Table 1), due to inadequate cell number, as a study showed that instrument calibration problems and limited cell number might have an impact on flow cytometry analysis. 41 In conclusion, at the end of culture hADSCs showed similar profile and proliferation, when cultured in F1, F2, and F3 containing medium.…”

Section: Discussionmentioning

confidence: 98%

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A Proliferation and surface marker characterization of adipose stem cells after culture in various processed outdated platelet lysate containing media

Sari¹,

Luviah²,

Purwoko³

et al. 2017

IJPTR

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: Human adipose derived stem cells (hADSCs) can be cultured in outdated platelet lysate (PL) containing medium. Processing of thrombocyte concentrate to get PL can be done by various freeze-thaw cycles. There was no information whether various processed PL containing media gave the same proliferation potential and surface marker expressions. Therefore, the aim of this study was to know the proliferation and surface marker characteristics of hADSCs after expansion in various processed PL containing media. hADCSs were cultured in various processed PL containing media, namely F1, F2, and F3, where the numbers denoted once, twice and three times freeze-thaw cycles. Proliferations were measured on day-2, day-4, day-7, and day-10. Positive surface markers were CD90, CD73, and CD105, while negative markers were a cocktail of CD34, CD45, CD11b, CD19, and HLA-DR. Difference in proliferations and surface marker expressions between F1, F2, and F3 were analyzed by one-way ANOVA. We found that cell proliferation in F1, F2, and F3 showed no significant difference on day-7 and day-10. The expression levels of CD90 and CD 105 in F1, F2, and F3 were all above 90%, which correspond with mesenchymal stem cells according to the requirement of International Society for Cell Therapy (ISCT). However, CD73 showed highest (97%) expression on F2 and lowest on F3 (81.8%). Negative marker range was 2.4-2.9%. In Conclusion, at the end of culture, hADSCs showed similar profile and proliferation, when cultured in F1, F2, and F3.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 98%

A Proliferation and surface marker characterization of adipose stem cells after culture in various processed outdated platelet lysate containing media

Sari¹,

Luviah²,

Purwoko³

et al. 2017

IJPTR

View full text Add to dashboard Cite

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“…Overall, VBD allowed for either, maintenance of MCS's fibroblast-like morphology (17,43,57), high proliferation (58-60), high colony-formation ability (52,61,62) and maintenance of multipotency (63)(64)(65)(66)(67). A linear dose-dependent response regarding medium concentration was not observed for evaluated VBD (54,56,68).…”

Section: Resultsmentioning

confidence: 93%

“…HPL presented a high growth factors and cytokines content (74)(75)(76)(77). HPL seemed to be better than FBS for ex vivo MSC expansion (52,72,78,79) since HPL-expanded MSC did not present telomerase shortening (68).…”

Section: Resultsmentioning

confidence: 99%

“…Overall, different protocols were developed and tested to obtain a FBS substitute able to maintain MSC in vitro, preserving stemness and multipotency (103,105). VBD were promissors as FBS substitutes, since senescence (68,106) or Karyotype/ chromosomal alteration were not detected in MSC expanded in VBD (13,23,(107)(108)(109). In addition, clinical studies strengthened such evidence by reporting none malign transformation in vivo (96,99,101,102).…”

Section: Discussionmentioning

confidence: 99%

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Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

et al. 2017

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Although the biological properties of mesenchymal stem cells (MSC) are well-characterized in vitro, MSC clinical application is still far away to be achieved, mainly due to the need of xenogeneic substances for cell expansion, such as fetal bovine serum (FBS). FBS presents risks regarding pathogens transmissions and internalization of animal's proteins, which can unleash antigenic responses in patients after MSC implantation. A wide range of venous blood derivatives (VBD) has been reported as FBS substitutes showing promising results. Thus, the aim of this study was to conduct a systematic scoping review to analyze whether VBD are effective FBS substitutes for MSC ex vivo expansion. The search was performed in SciVerse Scopus TM , PubMed, Web of Science TM , BIREME, Cochrane library up to January 2016. The keywords were selected using MeSH and entry terms. Two independent reviewers scrutinized the records obtained considering specific inclusion criteria. The included studies were evaluated in accordance with a modified Arksey and O' Malley's framework. From 184 found studies, 90 were included. Bone marrow mesenchymal stem cells (BMMSC) were presented in most of these studies. Overall, VBD allowed for either, maintenance of MCS's fibroblast-like morphology, high proliferation, high colony-formation ability and maintenance of multipotency. Besides. MSC expanded in VBD supplements presented higher mitogen activity than FBS. VBD seems to be excellent xeno-free serum for ex vivo expansion of mesenchymal stem cells. However, an accentuated heterogeneity was observed between the carried out protocols for VBD isolation did not allowing for direct comparisons between the included studies.

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Mesenchymal stromal cells; a new horizon in regenerative medicine

Tavakoli

Jafarbeigloo

Shariati

et al. 2020

Journal Cellular Physiology

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In recent decades, mesenchymal stromal cells (MSCs) biomedical utilizing has attracted worldwide growing attention. After the first report of the human MSCs obtaining from the bone marrow (BM) tissue, these cells were isolated from wide types of the other tissues, ranging from adipose tissue to dental pulp. Their specific characteristics, comprising self‐renewality, multipotency, and availability accompanied by their immunomodulatory properties and little ethical concern denote their importance in the context of regenerative medicine. Considering preclinical studies, MSCs can modify immune reactions during tissue repair and restoration, providing suitable milieu for tissue recovery; on the other hand, they can be differentiated into comprehensive types of the body cells, such as osteoblast, chondrocyte, hepatocyte, cardiomyocyte, fibroblast, and neural cells. Though a large number of studies have investigated MSCs capacities in regenerative medicine in varied animal models, the oncogenic capability of unregulated MSCs differentiation must be more assessed to enable their application in the clinic. In the current review, we provide a brief overview of MSCs sources, isolation, and expansion as well as immunomodulatory activities. More important, we try to collect and discuss recent preclinical and clinical research and evaluate current challenges in the context of the MSC‐based cell therapy for regenerative medicine.

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Human platelet lysate stimulates high-passage and senescent human multipotent mesenchymal stromal cell growth and rejuvenation in vitro

Cited by 80 publications

References 50 publications

A Proliferation and surface marker characterization of adipose stem cells after culture in various processed outdated platelet lysate containing media

A Proliferation and surface marker characterization of adipose stem cells after culture in various processed outdated platelet lysate containing media

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

Mesenchymal stromal cells; a new horizon in regenerative medicine

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