Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

Abstract

Although the biological properties of mesenchymal stem cells (MSC) are well-characterized in vitro, MSC clinical application is still far away to be achieved, mainly due to the need of xenogeneic substances for cell expansion, such as fetal bovine serum (FBS). FBS presents risks regarding pathogens transmissions and internalization of animal's proteins, which can unleash antigenic responses in patients after MSC implantation. A wide range of venous blood derivatives (VBD) has been reported as FBS substitutes showing promising results. Thus, the aim of this study was to conduct a systematic scoping review to analyze whether VBD are effective FBS substitutes for MSC ex vivo expansion. The search was performed in SciVerse Scopus TM , PubMed, Web of Science TM , BIREME, Cochrane library up to January 2016. The keywords were selected using MeSH and entry terms. Two independent reviewers scrutinized the records obtained considering specific inclusion criteria. The included studies were evaluated in accordance with a modified Arksey and O' Malley's framework. From 184 found studies, 90 were included. Bone marrow mesenchymal stem cells (BMMSC) were presented in most of these studies. Overall, VBD allowed for either, maintenance of MCS's fibroblast-like morphology, high proliferation, high colony-formation ability and maintenance of multipotency. Besides. MSC expanded in VBD supplements presented higher mitogen activity than FBS. VBD seems to be excellent xeno-free serum for ex vivo expansion of mesenchymal stem cells. However, an accentuated heterogeneity was observed between the carried out protocols for VBD isolation did not allowing for direct comparisons between the included studies.

Hydroxyapatite Synthesis and Covering of Titanium Surfaces by Dip-Coating Method

“…Thick or irregular coating layers might modify the surface roughness and consequently produce heterogeneous textures. Irregular films are not desirable since could negatively interfere with cell adherence, differentiation, and consequently, on osseointegration [26,33,35,36].…”

Bone, Periodontal and Dental Pulp Regeneration in Dentistry: A Systematic Scoping Review

“…GF-MT reduces costs since there is no need for an additional intervention for cell isolation. Furthermore, GF-MT presents insignificant risk of contamination since does not require the addition of xenogeneic substances as medium culture and its supplementation (28,200). PRP and PRF have been reported as extracellular matrix mimetics being both able to guide cell behavior showing interesting results combined with SC-BT and C-BT therapy (41,46,52,55,64,66,67,75).…”

“…The present scoping study was conducted following the modified five-stage framework suggested by Arksey and O'Malley (27) named Scoping Review. Newly, numerous papers use Scoping Review to state the actual knowledge in a particular area to provide a concise qualitative analysis (25,28). About the search strategy, the scoping study is indistinguishable to systematic reviews, directing a systematic and reproducible search.…”

“…Injectable scaffolds appear as an interesting option facilitating the clinical application of regenerative therapies (39,41,45,55,64) even as the use of PRP, when combined with biomaterials can facilitate the clinical manipulation of the materials for graft (182,198). Other possibility related, the replacement of xenogeneic fetal bovine serum by autologous human serum, has been reported as an option to reduce exogenous agents to ex vivo cell expansion (28,62,63).…”

Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications

“…These medications should be biocompatible, not carcinogenic, and/or genotoxic to periradicular tissue since they remain in contact with the periodontium 6 . Products of materials degradation in contact with existing cells in periradicular tissue can result in chemical irritation and inflammation, with a huge variety of chemical inflammatory mediators, which in high levels can cause tissue destruction and delay on healing process [7][8][9] . Therefore, these medications should present the ability to induce repair in the injured area without interfering with osteogenesis and cementogenesis [9][10][11] .…”