Human papillomavirus type-distribution in cervical cancer in China: the importance of HPV 16 and 18

Abstract

Prophylactic vaccination against HPV 16 and 18 has the potential for effective prevention of high-grade precancer (cervical intraepithelial neoplasia [CIN)] 2/3) and ICC caused by these viruses (globally 50 and 70%, respectively) when employed in women prior to starting sexual activity. To provide data for decisions on HPV vaccination in China, we determined HPV type-distribution in ICC and CIN 2/3 from women of different regions within China. A multicenter study was conducted by randomized sampling of paraffin blocks of 664 ICC (630 squamous cell carcinoma [SCC]; 34 adenocarcinoma [ADC]), 569 CIN 2/3 cases from seven regions of China. Histological diagnosis was confirmed in 1,233 cases by consensus review. HPV DNA was detected using the SPF10 LiPA25 version 1 assay. HPV prevalence was 97.6% in SCC, 85.3% in adenocarcinoma, and 98.9% in CIN 2/3. HPV 16 (76.7%) and HPV 18 (7.8%) were the most common, together accounting for 84.5% of SCC, followed by HPV 31 (3.2%), HPV 52 (2.2%), and HPV 58 (2.2%). HPV positivity in SCC did not differ notably by region. However, SCC cases from women <or=34 years had higher HPV 16 positivity than women over 50 years, among whom HPV 52, 58, and 39 were more common. HPV 16 and 18 were under-represented, whereas HPV 31, 52, and 58 were over-represented in CIN2/3 compared to SCC. The potential impact of vaccines against oncogenic HPV types 16 and 18 is estimated to be high (84.5%) against total SCC. These data are critical for China's future evaluation of the cost-effectiveness of current cervical cancer vaccines and of HPV-based screening guidelines.

Human Papillomavirus Type 16 Variant Analysis of E6, E7, and L1 Genes and Long Control Region in Identification of Cervical Carcinomas in Patients in Northeast China

“…As the most prevalent genotype, the prevalences of HPV 16 among different geographical regions in China were similar (7,42,45,56). However, the distribution of HPV 16 variants in China was studied less.…”

Human Papillomavirus Infection and Prognostic Predictors in Patients with Oropharyngeal Squamous Cell Carcinoma

“…PCR on the diluted DNA was applied to amplify HPV DNA and then the generic amplification products were detected by DNA enzyme immunoassay (DEIA). HPV positive specimens were typed by reverse hybridization line probe assay SPF10 LiPA25 (version 1) (Kleter et al, 1999;Chen et al, 2009). HPV-DNA testing of paraffin sections is especially vulnerable to cross-contamination.…”

Detection of Human Papilloma Virus Type 16 in Epithelial Ovarian Tumors Samples

“…There are more than 100 known types of HPV of which at least 15 HPV types have been ranked as high pathogenic (HPV 16,18,35,31,39,51,45,56,52,66,59, 69, 68, 82, and 73), whiles those related to moderate warts of genitals and skin are low pathogenic types (HPV 6,11,43,42,40,44,61,54,72,70,81) for the cancer of the genital region in women (6,7). Infection with carcinogenic HPV types is noted as the major reason for the increase of all invasive cancers of cervical region and the neoplasia of intraepithelial cervical carcinoma (8)(9)(10). OCa is the utmost usual abnormality of the females in the cervix (11,12) and the most lethal of the gynecologic malignancies with 16% -51% globally.…”