Detection of Human Papilloma Virus Type 16 in Epithelial Ovarian Tumors Samples

Abstract

Background: Ovarian carcinoma is the most common malignancy in women and is a cancer with a 15% -50% prevalence in the world. Human papilloma virus (HPV) is considered a factor in cervical and ovarian cancer (OCa) and is related to squamous cell carcinoma in the cervical region. The effect of fixed infection may cause chronic inflammation, in the cancer of ovaries it has received very rare attention, although a background of pelvic inflammatory disease (PID) in a case-control study is associated with a higher risk for ovarian carcinoma. HPV-16 is one type of HPV and the most common and important cause of cervical carcinoma in the developed world. Objectives: The aim of this investigation was to evaluate the incidence of HPV-16 in patients with OCa who referred to Imam Hossein hospital of Shahid Beheshti University of Medical Sciences, Tehran, Iran. Methods: In this case-control study that was conducted since May 2015 to October 2015 in Tehran, 140 samples were studied which were obtained from patients with OCa. After obtaining the samples from OCa tissue by a pathologist, for DNA extraction, samples were transferred to the laboratory of the university. DNA was extracted with Kit (intron biotechnology Co. Korea) according to the manufacturer's instructions. To confirm the presence of HPV-16 in the samples of OCa, specific primers for the L1 gene of HPV were designed and standard PCR method was used for the detection of HPV. PCR product was sequenced to confirm the presence of HPV-16. Results: Out of 140 samples of OCa, 70 (50%) samples were malignant cancer and 70 (50%) were benign cancer as the control group. Out of 70 malignant samples 25 (36.0%) were HPV-16 positive. 2.8% of the tissue samples of the control group were positive for HPV-16. Conclusions: These results show a feasible role of HPV-16 in the carcinogenesis of OCa. According to these results, infection with HPV may play a relative role in the spread of OCa or it could comfort its development.

Association of Human Papilloma Virus and Epstein-Barr Virus with Ovarian Cancer in Shiraz, Southwestern Iran

“…The results of present study showed that the frequency of HPV infection in malignant tumor groups was significantly higher than benign and the control groups. In agreement with our results, Dadashi et al reported that the frequency of HPV in malignant ovarian tissue was significantly higher than the tissues from benign participants (13) . Moreover, Al-Shabanah et al reported that 42% and 8% of the ovarian carcinoma samples and normal adjacent tissues as the control group had positive result for HPV, which was statistically IRANIAN JOURNAL OF PATHOLOGY significant (22).…”

“…Although the association of HPV with cervical cancer is well defined (8), the role of HPV in other cancers, such as esophageal squamous cell carcinoma, lung, prostate, breast, skin, colorectal, urinary tract and ovarian cancers has not been proven conclusively (9)(10)(11) . In this regards, (13) . EBV is the first human virus to be assigned as carcinogenic pathogen .…”

“…Although the association of HPV with cervical cancer is well defined ( 8 ), the role of HPV in other cancers, such as esophageal squamous cell carcinoma, lung, prostate, breast, skin, colorectal, urinary tract and ovarian cancers has not been proven conclusively ( 9 - 11 ). In this regards, in Iran, Farzane et al could not find any association between HPV infection and ovarian cancer ( 12 ), but Dadashi et al suggested that HPV infection is associated with ovarian cancer ( 13 ).…”

Viruses as key modulators of the TGF‐β pathway; a double‐edged sword involved in cancer

“…The majority of cervical cancer is mediated by HPV16 and HPV18 encoding the E6 and E7 oncoproteins. 50,51 There is a close relationship between HPV infection and the TGF-β pathway in cervical tissues; investigations have reported upmodulated TGF-β levels in HPV-positive cervical cancers compared with HPV-negative ones and have suggested a positive correlation between the expression levels of TGF-β and the HPV E6/E7 oncogenes, which may be due to trans-regulatory functions of the E6/E7 oncoproteins, so that, HPV16 E6/E7 have been shown to enhance TGF-β promoter activity by interacting with an Sp1-binding site placed in the TGF-β core promoter. 52 However, a prominent aspect of malignant transformation in cervical epithelial cells is the progressive loss of TGF-β responsiveness.…”

Prevalence of Human Papilloma Virus in Patients With Colorectal Cancer: A Systematic Review and Meta-analysis

“…Human papillomavirus (HPV) is a small virus with doublestranded DNA that is a member of the Papillomaviridae family, namely, a group with a well-recognized etiological role in cervical and vaginal cancer. [1][2][3] There is evidence for the presence of more than 100 types of HPV in the medical literature. In addition, 15 types of HPV (16,18,35,31,39,51,45,56,52,66,59, 69, 68, 82, and 73) are considered to be highly involved in the pathogenesis of genital cancer in women while HPVs responsible for the warts of genitals and skin (HPV 6,11,43,42,40,44,61,54,72,70, and 81) have lower pathogenicity.…”