Human Papillomavirus Type 16 Variant Analysis of E6, E7, and L1 Genes and Long Control Region in Identification of Cervical Carcinomas in Patients in Northeast China

Shang, Qing; Wang, Yan; Fang, Yong; Wei, Lanlan; Chen, Sijia; Sun, Yuhui; Li, Baoxin; Zhang, Fengmin; Gu, Huiying

doi:10.1128/jcm.02203-10

Cited by 43 publications

(25 citation statements)

References 51 publications

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“…The mutation most frequently observed in LCR was G7521A in the binding site of the transcription factor YY1 (Pande et al, 2008). In this study, in accordance to other authors (Yamada et al, 1997;Schmidt et al, 2001;Kammer et al, 2002;Pande et al, 2008;Shang et al, 2011), the mutation most frequently detected in the LCR region was G7521A, found in the African lineages and most European ones. Thus, only 20% of analyzed samples presented homology with the prototype sequence.…”

Section: 1151 Mutation Detection Of E6 and Lcr Genes From Hpv 16 Asssupporting

confidence: 80%

Mutation Detection of E6 and LCR Genes from HPV 16 Associated with Carcinogenesis

Mosmann¹,

Monetti²,

Frutos³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Human papillomavirus (HPV) is responsible for one of the most frequent sexually transmitted infections. The first phylogenetic analysis was based on a LCR region fragment. Nowadays, 4 variants are known: African (Af-1, Af-2), Asian-American (AA) and European (E). However the existence of sub-lineages of the European variant havs been proposed, specific mutations in the E6 and LCR sequences being possibly related to persistent viral infections. The aim of this study was a phylogenetic study of HPV16 sequences of endocervical samples from Córdoba, in order to detect the circulating lineages and analyze the presence of mutations that could be correlated with malignant disease. The phylogenetic analysis determined that 86% of the samples belonged to the E variant, 7% to AF-1 and the remaining 7% to AF-2. The most frequent mutation in LCR sequences was G7521A, in 80% of the analyzed samples; it affects the binding site of a transcription factor that could contribute to carcinogenesis. In the E6 sequences, the most common mutation was T350G (L83V), detected in 67% of the samples, associated with increased risk of persistent infection. The high detection rate of the European lineage correlated with patterns of human migration. This study emphasizes the importance of recognizing circulating lineages, as well as the detection of mutations associated with high-grade neoplastic lesions that could be correlated to the development of carcinogenic lesions.

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Section: 1151 Mutation Detection Of E6 and Lcr Genes From Hpv 16 Asssupporting

confidence: 80%

Mutation Detection of E6 and LCR Genes from HPV 16 Associated with Carcinogenesis

Mosmann¹,

Monetti²,

Frutos³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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“…Previous studies have shown that some nucleotide substitutions have remarkable importance in pathogenicity [11,20,21,22]. In the present study, 16 nucleotide variations were detected.…”

Section: Discussionsupporting

confidence: 56%

Human Papillomavirus 16 Variants May Be Identified by <b><i>E6 </i></b>Gene Analysis

Fontecha

Basaras²,

Arrese³

et al. 2015

Intervirology

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Aims: The aims of the study were (1) to characterize the genetic variability of human papillomavirus (HPV) genotype 16 in the E6 region when this genotype is present in multiple infection samples, (2) to assess the prevalence of variants in our region and (3) to analyze the relationship between variants, patients' ages and pathology. Methods: The Clinical Microbiology and Infection Control Department analyzed samples which were positive for genotype 16 and other genotypes from 2007 to 2013. Variants were assigned to European, Euro-German, Asian, Asian-American or African lineage by sequence analysis. The relationship among variants, age and different types of lesion was studied. Results: In HPV-16 sequence analysis, the European variant was detected in 85.10% of samples, the Asian-American in 7.80%, the African in 4.25% and the Euro-German in 2.83% of specimens. Sequence genetic variability showed 16 nucleotide substitutions. Moreover, non-European variants were mainly found in old women and in isolates from high-grade squamous intraepithelial lesions since European variants were mainly detected in negative cytologies. Conclusion: Multiple infections may take effect on nucleotide substitution and the appearance of recombinant samples. Single gene analysis makes it impossible to detect recombination which has a great influence on drug response and vaccine strategies. Thus, E6 gene analysis would be enough to identify HPV-16 intratypic variants but not to confirm the results.

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“…Previous studies have reported the proportion of HPV16 variants in some areas in China, and Asian and European lineages are thought to be the two major HPV16 strains in northeastern [16], central [17] and southern China [18]. However, most of these studies were based on the E6 genes or partial fragments of HPV16.…”

Section: Introductionmentioning

confidence: 94%

Physical Status and Variant Analysis of Human Papillomavirus 16 in Women from Shanghai

Guo

Zhu

et al. 2015

Gynecol Obstet Invest

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Background/Aims: Human papillomavirus type 16 (HPV16) is the cause of more than half of all cases of cervical cancer. Genetic mutations in HPV16 and the integration of HPV16 DNA in the human genome are considered important genetic changes in cervical lesion progression. However, limited data concerning HPV16 lineages and physical integration status have been reported for Shanghai, China. The current study analyzed the genetic mutations in complete HPV16 genomes and the physical integration status of HPV16 DNA. Methods: A total of 30 samples of cervical exfoliated cells from patients with HPV16 infection were collected. The entire HPV16 genome was isolated, amplified by PCR and directly sequenced. The physical integration status was determined by 3′RACE nested PCR. Results: A total of 13 integration sites were identified, including 9 in common fragile sites and 1 not close to any fragile sites. Phylogenetic analysis identified two HPV lineages: the European (E) lineage and the East Asian (EA) lineage. Amino acid changes of D25E and N29S were the most common variations across the genome. The HPV16 early genes E1 and E7 and the late gene L1 tended to be highly conserved, whereas the early genes E2, E4 and E6 were more variable. Furthermore, 10 novel variations were identified in this study, which led to the 3 amino acid changes of S23I in E2 and E244K and T269I in E2/E4. Conclusion: Integrated HPV16 viruses were detected in all stages of cervical samples. Many variants in E2, E4, E7, and the long control region co-varied with E6 variations and helped to define the HPV16 lineages.

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Human Papillomavirus Type 16 Variant Analysis of E6, E7, and L1 Genes and Long Control Region in Identification of Cervical Carcinomas in Patients in Northeast China

Cited by 43 publications

References 51 publications

Mutation Detection of E6 and LCR Genes from HPV 16 Associated with Carcinogenesis

Mutation Detection of E6 and LCR Genes from HPV 16 Associated with Carcinogenesis

Human Papillomavirus 16 Variants May Be Identified by <b><i>E6 </i></b>Gene Analysis

Physical Status and Variant Analysis of Human Papillomavirus 16 in Women from Shanghai

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