Human mesenchymal stem cells alter macrophage phenotype and promote regeneration via homing to the kidney following ischemia-reperfusion injury

Wise, Andrea F; Williams, T.; Kiewiet, Mensiena B. G.; Payne, Natalie Lisa; Siatskas, Christopher; Samuel, Chrishan S.; Ricardo, Sharon D.

doi:10.1152/ajprenal.00675.2013

Cited by 114 publications

(102 citation statements)

References 72 publications

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“…Moreover, our results showed that the liver was the main organ retaining exogenous macrophages (Fig. 3B-C), as previously described by other authors[14, 19, 20]. Once the detection of the infused macrophages in the kidney was confirmed, we next isolated them by cell sorting two days after the infusion to analyze their phenotype and function.…”

Section: Resultssupporting

confidence: 82%

Macrophage Overexpressing NGAL Ameliorated Kidney Fibrosis in the UUO Mice Model

Guiteras

Solà

Flaquer

et al. 2017

Cell Physiol Biochem

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Background/Aims: Alternatively activated macrophages (AAM) have regenerative and anti-inflammatory characteristics. Here, we sought to evaluate whether AAM cell therapy reduces renal inflammation and fibrosis in the unilateral ureteral obstruction (UUO) mice model. Methods: We stabilized macrophages by adenoviral vector NGAL (Neutrophil gelatinase-associated lipocalin-2) and infused them into UUO mice. To ascertain whether macrophages were capable of reaching the obstructed kidney, macrophages were stained and detected by in vivo cell tracking. Results: We demonstrated that some infused macrophages reached the obstructed kidney and that infusion of macrophages overexpressing NGAL was associated with reduced kidney interstitial fibrosis and inflammation. This therapeutic effect was mainly associated with the phenotype and function preservation of the transferred macrophages isolated from the obstructed kidney Conclusions: Macrophage plasticity is a major hurdle for achieving macrophage therapy success in chronic nephropathies and could be overcome by transferring lipocalin-2.

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Section: Resultssupporting

confidence: 82%

Macrophage Overexpressing NGAL Ameliorated Kidney Fibrosis in the UUO Mice Model

Guiteras

Solà

Flaquer

et al. 2017

Cell Physiol Biochem

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“…This is also supported by the in vitro finding that macrophages stimulated with MSCs show an increase in the M2 phenotype, and the transfer of MSC-cocultured macrophages into the macrophage depletion mice inhibits renal injury [41]. Consistently, the role of MSCs in M2 polarization was also confirmed in IRI [42]. …”

Section: Regulatory Mechanisms Of Macrophage Polarization In Kidney Dmentioning

confidence: 58%

Macrophage Phenotype in Kidney Injury and Repair

Meng

Tang

et al. 2015

Kidney Dis

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Background: Glomerular and interstitial macrophage infiltration is a feature for both the acute and chronic kidney diseases. Macrophages have been shown to play a diverse role in kidney injury and repair. Thus, macrophages may be a key cell type in acute and chronic kidney injury and repair. Summary and Key Messages: During renal inflammation, circulating monocytes are recruited and then become activated and polarized. By adapting to the local microenvironment, macrophages can differentiate into different phenotypes and function as a double-bladed sword in different stages of kidney disease. In general, M1 macrophages play a pathogenic role in boosting inflammatory renal injury, whereas M2 macrophages exert an anti-inflammatory and wound healing (or profibrotic) role during renal repair. In this review, we highlight the phenotypic polarization of macrophages in renal diseases and dissect their distinct functions in renal injury and repair processes, respectively. Moreover, the current understanding of regulatory mechanisms on the phenotypic switch and macrophage-related therapy are also intensively discussed.

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“…As a result, these cells can be efficient carriers for anti-cancer therapeutic agents' delivery to target tissue, a critical feature of achieving therapeutic efficacy. Moreover, these cells have shown a strong chemotactic response to injuries (14,41). Thus, OECs and BMS cells may be used as tumor-selective targeting carriers for the delivery of anti-cancer agents and cytotoxic genes toward the tumor.…”

Section: █ Discussionmentioning

confidence: 99%

Effects of the two types of human cells on outgrowth of human glioma in rats

Abdi

Eskandary²,

Nematollahi-Mahani³

2016

Turkish Neurosurgery

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In early cell-therapy studies (in the treatment of cancer), Aboody et al. (2) showed significant migration of neural stem cells (NSCs) towards the tumor area. In line with this, types of stem cells, and especially mesenchymal stem cells (MSCs), that have been evaluated in several tumor models including breast, prostate, and ovarian cancer are used (11,15,23). However, the data are not adequate and some are contradictory to each other.Olfactory ensheathing cell (OEC) is a neural stem cell type of the glial family like astrocytes and Schwann cells (35). In the recent decade, several reports have provided evidence that █ INTRODuCTION G lioblastoma (GBM) is one of the primary malignant brain tumors that occur most frequently. Nearly, all patients with GBM die within 12-15 months of the initial diagnosis (3,7,25). Therapies are still limited due to the ability of the blood brain barrier (BBB) to prevent the crossing of a broad range of anti-cancer agents (10,19). Therefore, the cure of GBM is a major concern. Accordingly, there is a lot of interest in devising novel approaches, such as stem cell therapy, to treat patients with GBM (10,12).AIm: Glioblastoma multiforme (GBM) is one of the malignant brain tumors that occur most frequently. Despite advances in therapy techniques, the cure of GBM is a major concern. Accordingly, there is a lot of interest in devising novel approaches, such as stem cell therapy, to treat patients with GBM. The aim of this study was to investigate the effects of human bone marrow stem (BMS) cells as well as human olfactory ensheathing cells (OECs) on the outgrowth of U87 glioma in rats. mATERIAl and mEThODS:OECs and BMS cells were obtained from volunteers. After verification of the stem cell type by flow cytometry and immunocytochemistry (ICC), cells were labeled and injected into human glioma-bearing rats. Magnetic resonance imaging (MRI), Hematoxylin and Eosin (H&E), and Immunohistochemistry (IHC) were utilized to assess the properties of the groups. RESulTS:We found extensive migration and homing of the OECs and BMS cells towards the tumor area. H&E and IHC staining indicated that the grafted OECs survived and prevented the development of glioma. BMS cells supported proliferation and new vessel formation, and metastasis in glioma tissue. CONCluSION:OECs and BMS cells can pass the blood brain barrier and reach the glioma mass. Therefore, this approach can be a potentially powerful method for the delivery of therapeutic agents to malignant brain tumors. In addition, these cells may be genetically modified in order to specifically express tumor-suppressive factors.

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Human mesenchymal stem cells alter macrophage phenotype and promote regeneration via homing to the kidney following ischemia-reperfusion injury

Cited by 114 publications

References 72 publications

Macrophage Overexpressing NGAL Ameliorated Kidney Fibrosis in the UUO Mice Model

Macrophage Overexpressing NGAL Ameliorated Kidney Fibrosis in the UUO Mice Model

Macrophage Phenotype in Kidney Injury and Repair

Effects of the two types of human cells on outgrowth of human glioma in rats

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