Human herpesvirus type 8 DNA sequences in biological samples of HIV-positive and negative individuals in Sicily

Viviano, Enza; Vitale, Francesco; Ajello, Francesca; Perna, Anna Maria; Villafrate, M. R.; Bonura, Filippa; Aricò, Mario; Mazzola, Giovanni; Romano, Nino

doi:10.1097/00002030-199705000-00008

Cited by 81 publications

(50 citation statements)

References 24 publications

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“…This conclusion is supported by other studies showing a low rate of parenteral transmission of HHV-8 (8,12,13). The lack of any statistically significant association between HHV-8 seropositivity and blood transfusion or years of dialysis suggests that HHV-8 transmission may involve other routes (4,5,6,10,15,22).…”

Section: Discussionsupporting

confidence: 72%

“…This rate was statistically indistinguishable from the rate (14.9%) observed in controls. The observed rate of seropositivity in pretransplant patients was higher than rates reported in other European countries (8,19) and can be explained by the higher-thanaverage HHV-8 seroprevalence in the general population of central and southern Italy (5,14,22,23). The data suggest that HHV-8 seroprevalence is unaffected by pretransplant therapeutic regimens.…”

Section: Discussionmentioning

confidence: 54%

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Kaposi's Sarcoma Associated with Previous Human Herpesvirus 8 Infection in Kidney Transplant Recipients

et al. 2001

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This study investigates the prevalence of human herpesvirus 8 (HHV-8) infection in kidney transplant patients, evaluating the risk of HHV-8 transmission via transplantation and the association between pre-and posttransplantation HHV-8 infection and the subsequent development of Kaposi's sarcoma (KS). Immunofluorescence and an enzyme immunoassay were used to determine HHV-8 seroprevalence in 175 patients awaiting kidney transplantation and 215 controls who were attending our clinic for other reasons. All patients in the study came from central or southern Italy. Seroprevalence was similar in both groups (14.8 versus 14.9%), with no significant difference between the rates for male and female patients. Of the 175 patients, 100 were tested for anti-HHV-8 antibodies at various times during follow-up. During follow-up, seroprevalence increased from 12% on the date of transplantation to 26%. This increase was paralleled by an age-related increase in seroprevalence in the control group. During follow-up from 3 months to 10 years after transplantation, KS was diagnosed in seven patients (4.0%). Six of these patients were positive for HHV-8 prior to transplantation. Overall, 23.0% of patients who were HHV-8 positive before transplantation developed KS, whereas only 0.7% of seronegative patients developed the disease (relative risk, 34.4; 95% confidence interval, 4.31 to 274.0). This finding suggests that the key risk factor for KS is infection prior to transplantation and that antibody detection in patients awaiting transplantation could be useful in identifying patients at high risk for KS. In patients from geographic areas with a high prevalence of HHV-8, serological tests on donors may be less important.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 54%

Kaposi's Sarcoma Associated with Previous Human Herpesvirus 8 Infection in Kidney Transplant Recipients

et al. 2001

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“…Similarly, semen samples from healthy US, UK, French and some Italian donors did not contain detectable KSHV (Howard et al, 1997 ;Lebbe! et al, 1997 b ;Gupta et al, 1996 ;Corbellino et al, 1996 a, b), with one exception (Lin et al, 1995), whereas KSHV was detected in 13-23 % of Italian semen donors from areas where classic KS appears more frequently (Monini et al, 1996 a, b ;Viviano et al, 1997 ;reviewed in Blackbourn & Levy, 1997).…”

Section: Epidemiology Of Kshvmentioning

confidence: 98%

“…Since these behavioural risk factors have previously been shown to increase the risk of KS developing in homosexual men (Beral et al, 1990) this observation also supports the notion that KSHV is indeed the ' KS agent '. The detection of KSHV in seminal fluid and prostates of KS patients (Monini et al, 1996 a, b ;Howard et al, 1997 ;Gupta et al, 1996 ;Corbellino et al, 1996 a, b), and occasionally in healthy men (Monini et al, 1996 a, b ;Viviano et al, 1997 ;Staskus et al, 1997), provides a possible explanation for the sexual transmission observed in seroepidemiological studies.…”

Section: Epidemiology Of Kshvmentioning

confidence: 99%

“…With few exceptions (Decker et al, 1996), KSHV genomes have not been detected by PCR in PBMC samples from UK, US or French healthy control individuals (Whitby et al, 1995 ;Moore et al, 1996 b ;Lefrere et al, 1996). They have, however, been detected in the lymphoid tissue from approximately 10 % of healthy Italian subjects (Bigoni et al, 1996 ;Viviano et al, 1997), in 10 % of PBMC from HIV-negative healthy individuals in The Gambia (Ariyoshi et al, 1998), in 8 % of febrile children in Zambia (Kasolo et al, 1997) and in 14 % of control tumour tissues from Ugandan patients . Similarly, semen samples from healthy US, UK, French and some Italian donors did not contain detectable KSHV (Howard et al, 1997 ;Lebbe!…”

Section: Epidemiology Of Kshvmentioning

confidence: 99%

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Kaposi's sarcoma-associated herpesvirus (human herpesvirus-8).

Schulz

1998

Journal of General Virology

237

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Human Herpesvirus 8 in Italian HIV-Seronegative Patients With Kaposi Sarcoma

Cattani

Capuano²,

Parola³

et al. 1998

Arch Dermatol

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To evaluate the prevalence of human herpesvirus 8 (HHV-8) DNA detection in a large series of human immunodeficiency virus-seronegative patients with and without Kaposi sarcoma (KS) from the central and southern regions of Italy where classic KS is prevalent. Design: Samples of lesional, peripheral unaffected, and distant normal skin and peripheral blood mononuclear cells (PBMCs) from 33 patients with KS and PBMCs from 42 control subjects were analyzed using single and nested polymerase chain reaction techniques for the presence of HHV-8 DNA. Patients: A total of 33 patients with KS not related to acquired immunodeficiency syndrome (26 patients with classic KS and 7 patients with iatrogenic KS) were studied. Furthermore, 2 control groups were enrolled. The first group consisted of 13 healthy volunteers, the second of 29 patients affected by different dermatological diseases. Results: Human herpesvirus 8 sequences were found in 100% of lesional and perilesional specimens, in 33% of the distant normal skin samples, and in 69.6% of the PBMCs from patients with KS. A possible correlation between HHV-8 DNA in PBMCs and the clinical stage of the disease was observed. Moreover, the prevalence of viral DNA in PBMCs from the total control group was 23.8%. No viral DNA was detected in tissue biopsy specimens taken from the control group. Conclusions: Our data suggest that HHV-8 could be a widespread virus, at least in Mediterranean regions where KS is more prevalent, such as southern and central Italy. As with other herpesviruses, it may be present lifelong in latent form somewhere in the body and may contribute to the pathogenesis of KS when other predisposing conditions are present.

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Human herpesvirus type 8 DNA sequences in biological samples of HIV-positive and negative individuals in Sicily

Cited by 81 publications

References 24 publications

Kaposi's Sarcoma Associated with Previous Human Herpesvirus 8 Infection in Kidney Transplant Recipients

Kaposi's Sarcoma Associated with Previous Human Herpesvirus 8 Infection in Kidney Transplant Recipients

Kaposi's sarcoma-associated herpesvirus (human herpesvirus-8).

Human Herpesvirus 8 in Italian HIV-Seronegative Patients With Kaposi Sarcoma

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