HPLC-PDA method for quinovic acid glycosides assay in Cat's claw (Uncaria tomentosa) associated with UPLC/Q-TOF–MS analysis

Medicinal uses, phytochemistry and pharmacology of the genus Uncaria

“…Among them, quinovic acid are commonly glycosylated at C-3, C-28, C-3,-28 or C-3,-27 positions with various sugar moieties, such as quinovose, rhamnose, glucose, fructose and galactose (Aquino et al, 1988). Characteristic pseudomolecular ion [MþH] þ at m/z 469 and [M þNa] þ at m/z 655, 817 and 979 in electrospray ionization LC-MS/MS method further indicate the presence of quinovic acid aglycine and mono-, di-and tri-glycosylated quinovic acid glycosides, respectively (Montoro et al, 2004;Pavei et al, 2012). Table 6 3.3.…”

Acute Toxicity and Determination of the Active Constituents of Aqueous Extract ofUncaria tomentosaBark inHyphessobrycon eques

“…The analyses were performed employing a previously validated HPLC-PDA method [7]. Briefly, a Sinergy Fusion RP-18 column (150 × 3.9 mm i.d., 5  μ m) (Phenomenex, USA) protected by a RP-18 guard column was used.…”

Antifungal activity of Uncaria tomentosa (Willd.) D.C. against resistant non-albicans Candida isolates

“…Quinovic acid glycoside content was analyzed using an HPLC-PDA method (Pavei et al, 2012) employing ␣-hederin (Sigma-Aldrich, USA) as external standard. The QAG content was calculated by the sum of individual concentrations of seven major peaks previously characterized as quinovic acid glycosides through UV and MS-MS data (Pavei et al, 2012), and expressed as g% (w/w) of ␣-hederin in dry material by mean of three determinations.…”

“…The QAG content was calculated by the sum of individual concentrations of seven major peaks previously characterized as quinovic acid glycosides through UV and MS-MS data (Pavei et al, 2012), and expressed as g% (w/w) of ␣-hederin in dry material by mean of three determinations.…”