Hormonal regulation of prooxidant-antioxidant homeostasis in gilts

Stoyanovskyy, V. G.; Усенко, С. О.; Shostya, A. M.; Kuzmenko, L. M.; Slynko, V. G.; Tenditnyk, V. S.

doi:10.32718/ujvas3-3.08

Cited by 4 publications

(4 citation statements)

References 5 publications

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“…It is oxidative degradation of lipids, occurring under the action of free radicals and is one of the main causes of damage to cellular membranes and further death of cells as a result of influence of reactive oxygen species. The main initiators of free radical oxidation are reactive oxygen species (ROS) that are able to grow under the influence of unsatisfactory factors, including heavy metals and cause oxidative stress (Stoyanovskyy et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors

Slobodian

Gutyj

Darmohray

et al. 2021

Regul. Mech. Biosyst.

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Prolonged ingress of heavy metals into the organisms of animals, even in low concentrations, causes a number of toxic impacts, affecting various organs and systems. The objective of this study was to research parameters of enzymatic and non-enzymatic links of the system of antioxidant protection of the organism of young cattle in the condition of lead-cadmium load and the effect of Metisevit Plus feed additive. Studies were conducted on the basis of the Private Agricultural Company “Ukraina”, located in Dubrovytsia district of Rivne Oblast, using 12 six-month old bulls of Ukrainian Black Pied Dairy breed divided into two groups, each consisting of 6 animals. Bulls of the control group received the standard diet. The bulls of the experimental group were fed with Metisevit Plus feed additive in the dose of 0.5 g/kg of feed. The contents of lead and cadmium in feed at the enterprise were determined to be high. According to the conducted studies, it was determined that in conditions of cadmium and lead load, there is a decrease in activity of glutathione link of the system of antioxidant protection of the organism of bulls. Feeding the bulls of the experimental group with Metisevit Plus feed additive for 30 days led to increase in the levels of non-enzymatic and enzymatic activities of the glutathione system. Significant increase in the level of reduced glutathione was recorded on days 30 and 40 of the experiment. Consumption of Metisevit Plus by the animals of the experimental group promoted increase in the activity of catalase and superoxide dismutase starting on the 20th day of the experiment. On days 30 and 40 of the experiment, the activity of the studied enzymes ranged within the physiological values. Therefore, the studies conducted on the bulls indicate that in the conditions of cadmium and lead load the feed additive Metisevit Plus fed to the bulls activated the system of antioxidant protection by increasing the levels of enzymatic and non-enzymatic links. When feeding bulls of the experimental group with Metisevit Plus feed additive, we determined significant decreases in diene conjugates and TBA-active products in their blood starting already on the 10th day of the experiment. On days 20 and 30 of the experiment, the level of diene conjugates in the blood of animals of the experimental group decreased by 14.5% and 24.0%. Efficiency of the feed additive may be explained by the effect of its constituents on the main pathogenic links of the pathological process caused by cadmium and lead. As indicated in the studies, liquidation of the syndrome of endogenous intoxication restores the functional condition of cellular membranes of the liver, protein-synthesizing function of the liver, increases the antioxidant status of the organisms of bulls. The studies we conducted confirm the expedience of using feed additive Metisevit Plus to prevent lead-cadmium toxicosis.

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Section: Discussionmentioning

confidence: 99%

Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors

Slobodian

Gutyj

Darmohray

et al. 2021

Regul. Mech. Biosyst.

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“…Дослідження саме глутатіонової системи за негативної дії важкими металами є актуальним не лише в контексті функціонування загальної антиоксидантної системи організму, а обумовлене також тим, що вона бере безпосередню участь у багатьох біохімічних механізмах детоксикації ліпофільних і гідрофільних ксенобіотиків (Stoyanovskyy et al, 2020;Vasylyev et al, 2021). Участь глутатіону і пов'язаних з ним систем у процесах біотрансформації та детоксикації ксенобіотиків визначає великою мірою стійкість організму до їх токсичної дії (Ivankiv et al, 2019;Grymak et al, 2020).…”

Section: вступunclassified

Influence of Metisevit Plus feed additive on the activity of the glutathione system of the body of bulls under conditions of man-caused load

et al. 2021

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Pollution of the environment with heavy metals due to artificial activities of the population has led to several problems for agricultural production. The work aims to study the influence of Metisevit Plus feed additive on the state of the glutathione system of the body of bulls under conditions of man-caused load. The research was conducted based on the private agricultural enterprise “Ukraine” Dubrovytsia district of Rivne region on 12 bulls six months old, Ukrainian black-and-white dairy breed, which was formed into two groups of 6 animals each: 1 group – control, bulls were on a standard diet; Group 2 – experimental, bulls were fed a feed additive “Metisevit plus” at a dose of 0.5 g/kg of feed. According to studies, cadmium and lead load reduce the activity of the glutathione link in the antioxidant defense system of bulls. Feeding the Metisevit Plus feed additive to the bulls of the experimental group for 30 days helped increase the level of non-enzymatic and enzymatic activity of the glutathione system. A probable increase in the level of reduced glutathione was found on 30 and 40 days of the experiment, were compared with the control group of animals, it increased by 7.2 and 7.1 %, respectively. In the study of the activity of glutathione peroxidase and glutathione reductase, it was found that in these study periods they ranged from 35.7 ± 1.61 and 35.3 ± 1.55 nmol NADPH/min per 1 mg of protein (P ≤ 0.01) and 1.67 ± 0.041 and 1.64 ± 0.039 nmol NADPH/min per 1 mg of protein (P ≤ 0.001). Thus, studies on bulls indicate that the feed additive Metisevit Plus, when fed to bulls, under a load of cadmium and lead, contributed to the activation of the glutathione antioxidant defense system by increasing the level of enzymatic and non-enzymatic units. Our studies confirm the feasibility of using a feed additive, Metisevit Plus, to prevent lead-cadmium toxicosis.

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“…It is worth noting that along with superoxide dismutase, catalase is also essential, which quickly splits peroxide into water and oxygen (Baglaj et al, 2011;Fedorenko, 2016). Superoxide dismutase and catalase protect the body from highly toxic oxygen radicals (Stoyanovskyy et al, 2020). Catalase is found in all tissues, containing about 10-6 M. In general, the effect of catalase is reduced to a decrease in the concentration of cytotoxic hydroxyl radicals (Duh & Vovk, 2010;Zhukova et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

The influence of the feed additive “Sylimevit” on the antioxidant protection of the body of dogs

Gutyj,

Petryshak,

Mylostyvyi

et al. 2023

SMLNUVMBA

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Under the influence of the pathological factor, there is a change in the intensity of lipid peroxidation, the accumulation in the blood of the concentration of products of free radical oxidation and reactive oxygen species, a decrease in the buffer capacity of the blood relative to the maintenance of optimal parameters of the intensity of free extreme reactions. The work aimed to investigate the effect of the feed additive “Sylimevit” on the antioxidant protection of the body of dogs after preventive deworming. 10 German Shepherd dogs aged 1–2 years were used for experimental research. It was established that when feeding the feed additive “Sylimevit” to dogs in the period after deworming, activation of the enzyme link of the antioxidant system occurs in the blood, which is indicated by an increase in the activity of catalase and superoxide dismutase in the blood serum of these animals and inhibition of lipid peroxidation processes (reduction of secondary and end products lipid peroxidation). On the 30th day of the experiment, the activity of catalase and superoxide dismutase in the blood of animals of the experimental group was the highest, and the level of lipid peroxidation products was the lowest. The obtained research results confirm the antioxidant properties of the Sylimevit feed additive. This may be because the composition of the drug includes milk thistle, methiphene, and vitamins, which in turn enhance the effect of each other and thereby inhibit the formation of radicals and the processes of lipid peroxidation. It is also worth noting the antioxidant properties of milk thistle, which according to the literature, also have similar properties. The complex effect of the indicated biologically active elements provided high hepatoprotective and antioxidant effects.

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Hormonal regulation of prooxidant-antioxidant homeostasis in gilts

Cited by 4 publications

References 5 publications

Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors

Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors

Influence of Metisevit Plus feed additive on the activity of the glutathione system of the body of bulls under conditions of man-caused load

The influence of the feed additive “Sylimevit” on the antioxidant protection of the body of dogs

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