Histomorphometric changes of the fore-stomach of lambs fed with diets containing spineless cactus genotypes resistant to Dactylopius sp.

Silva, Tomás Guilherme Pereira da; Batista, Ângela Maria Vieira; Guim, Adriana; Silva, Valdemiro Amaro da; Carvalho, Francisco Fernando Ramos de; Barros, Maria Edna Gomes de; Sousa, Daurivane Rodrigues; Silva, Suellen Maria Costa da

doi:10.1007/s11250-019-02129-0

Cited by 16 publications

(7 citation statements)

References 20 publications

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“…The rumen epithelium is composed of the stratum corneum, granular layer, spinous layer and basal layer and has important physiological functions such as VFA (Volatile fatty acid) metabolism, absorption, transportation and protection ( Gálfi et al, 1991 ; Steele et al, 2016 ). The stratum corneum has a small metabolic capacity and is an important protective barrier ( da Silva et al, 2020 ), and this study found that the thickness of the stratum corneum during the cold season is significantly higher than that in the warm season. This finding may be due to the rough quality and high fiber content of the cold season.…”

Section: Discussionmentioning

confidence: 72%

Multi-Omics Reveals That the Rumen Transcriptome, Microbiome, and Its Metabolome Co-regulate Cold Season Adaptability of Tibetan Sheep

Liu

Sha

et al. 2022

Front. Microbiol.

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Tibetan sheep can maintain a normal life and reproduce in harsh environments under extreme cold and lack of nutrition. However, the molecular and metabolic mechanisms underlying the adaptability of Tibetan sheep during the cold season are still unclear. Hence, we conducted a comprehensive analysis of rumen epithelial morphology, epithelial transcriptomics, microbiology and metabolomics in a Tibetan sheep model. The results showed that morphological structure of rumen epithelium of Tibetan sheep in cold season had adaptive changes. Transcriptomics analysis showed that the differential genes were primarily enriched in the PPAR signaling pathway (ko03320), legionellosis (ko05134), phagosome (ko04145), arginine and proline metabolism (ko00330), and metabolism of xenobiotics by cytochrome P450 (ko00980). Unique differential metabolites were identified in cold season, such as cynaroside A, sanguisorbin B and tryptophyl-valine, which were mainly enriched in arachidonic acid metabolism, arachidonic acid metabolism and linolenic acid metabolism pathways, and had certain correlation with microorganisms. Integrated transcriptome-metabolome-microbiome analysis showed that epithelial gene-GSTM3 expression was upregulated in the metabolism of xenobiotics by the cytochrome P450 pathway during the cold season, leading to the downregulation of some harmful metabolites; TLR5 gene expression was upregulated and CD14 gene expression was downregulated in the legionellosis pathway during the cold season. This study comprehensively described the interaction mechanism between the rumen host and microbes and their metabolites in grazing Tibetan sheep during the cold season. Rumen epithelial genes, microbiota and metabolites act together in some key pathways related to cold season adaptation.

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Section: Discussionmentioning

confidence: 72%

Multi-Omics Reveals That the Rumen Transcriptome, Microbiome, and Its Metabolome Co-regulate Cold Season Adaptability of Tibetan Sheep

Liu

Sha

et al. 2022

Front. Microbiol.

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“…The rumen epithelium is composed of the stratum corneum, granular layer, spinous layer and basal layer and has important physiological functions such as VFA metabolism, absorption, transportation and protection [21][22]. The stratum corneum has a small metabolic capacity and is an important protective barrier [23], and this study found that the thickness of the stratum corneum during the cold season is significantly higher than that in the warm season. This finding may be due to the rough quality and high fiber content of the cold season.…”

Section: Discussionmentioning

confidence: 64%

The Rumen Microbiome and Its Metabolome Together With the Host Transcriptome Act to Regulate the Cold Season Adaptation Mechanism of Grazing Tibetan Sheep

Liu

Sha

et al. 2021

Preprint

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BackgroundTibetan sheep are important ruminants on the Qinghai-Tibet Plateau. They can maintain a normal life and reproduce in harsh environments under extreme cold and low oxygen. However, the molecular and metabolic mechanisms underlying the adaptability of Tibetan sheep during the cold season are still unclear. Hence, we conducted a comprehensive analysis of rumen epithelial morphology, epithelial transcriptomics, microbiology and metabolomics in a Tibetan sheep model to understand the interaction between the rumen host and microbiota and their metabolites and to explore the potential regulatory mechanism of Tibetan sheep adaptability to the cold season of the plateau. ResultsMorphological analysis showed that the ruminal muscle layer thickness and nipple width of Tibetan sheep increased significantly during the cold season ( P <0.05), and the thickness of the stratum corneum, stratum granulosa and stratum spinous of the rumen epithelium increased significantly ( P <0.05). Transcriptomics analysis showed that the differential genes were primarily enriched in the PPAR signaling pathway (ko03320), legionellosis (ko05134), phagosome (ko04145), arginine and proline metabolism (ko00330), and metabolism of xenobiotics by cytochrome P450 (ko00980). Unique differential metabolites were identified in cold season, such as cynaroside A, sanguisorbin B and tryptophyl-valine, which were mainly enriched in arachidonic acid metabolism, arachidonic acid metabolism and linolenic acid metabolism pathways, and had certain correlation with microorganisms. Integrated transcriptome-metabolome-microbiome analysis showed that epithelial gene- GSTM3 expression was upregulated in the metabolism of xenobiotics by the cytochrome P450 pathway during the cold season, leading to the downregulation of some harmful metabolites; TLR5 gene expression was upregulated and CD14 gene expression was downregulated in the legionellosis pathway during the cold season. A large number of metabolites, such as glucosidic acid and vitamin A, were produced in the steroid hormone biosynthesis and retinol metabolism pathways. ConclusionThis study comprehensively described the interaction mechanism between the rumen host and microbes and their metabolites in grazing Tibetan sheep during the cold season. Under the stimulation of the cold plateau environment, the morphological structure of the rumen epithelium of Tibetan sheep undergoes adaptive changes. Rumen epithelial genes, microbiota and metabolites act together in some key pathways related to cold season adaptation.

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“…The quadratic effect observed for epithelial keratinization possibly re ects the decrease in the passage rate at the highest levels of CB inclusion, which induced a lower ow of the digest over the ruminal papillae, decreasing the abrasive effect on them, which led to a lower turnover rate cell in the outer layer of the epithelium, causing the keratin layer of the ruminal epithelium to decrease (Silva et al, 2020b). In the small intestine, the decrease in the passage rate possibly caused a linear decrease in the submucosal layer with the inclusion of CB.…”

Section: Discussionmentioning

confidence: 99%

Lambs Fed Diets Containing By-Product from Coconut Processing: Histomorphometry Characteristics in the Digestive and Renal Systems

Silva

Júnior

Guerra

et al. 2022

Preprint

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This study aimed to evaluate the histological characteristics in the digestive and renal systems of lambs fed diets containing coconut by-product (CB). A total of 35 male lambs with an initial weight of 16.9 ± 2.93 kg were distributed in a completely randomized design with five levels of CB in the diet (0; 4.8; 9.6; 14.4 and 19.2% in total dry matter). Samples of the liver, kidney, rumen, and intestine were histomorphometrically evaluated and the data were submitted to regression analysis, at a 5% error probability. The inclusion of CB linearly decreased the dry matter intake and caused a quadratic effect for the height of ruminal papillae, absorption area, epithelium thickness, as well as for average daily gain. The inclusion of CB linearly increased the mucous layer and reduced the submucosal layer, as well as promoted a decrease in goblet cells in the small intestine. The inclusion of BC did not influence hepatic glycogen, additionally, the histopathological examination did not reveal liver damage or congestion, vacuolization, and necrosis of the renal tissue. Therefore, our results indicate that CB can be included in lambs diet up to the level of 7.2% without causing changes in the histomorphometry characteristics of the gastrointestinal tract and changes in liver and kidney tissue that compromise animal performance.

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Histomorphometric changes of the fore-stomach of lambs fed with diets containing spineless cactus genotypes resistant to Dactylopius sp.

Cited by 16 publications

References 20 publications

Multi-Omics Reveals That the Rumen Transcriptome, Microbiome, and Its Metabolome Co-regulate Cold Season Adaptability of Tibetan Sheep

Multi-Omics Reveals That the Rumen Transcriptome, Microbiome, and Its Metabolome Co-regulate Cold Season Adaptability of Tibetan Sheep

The Rumen Microbiome and Its Metabolome Together With the Host Transcriptome Act to Regulate the Cold Season Adaptation Mechanism of Grazing Tibetan Sheep

Lambs Fed Diets Containing By-Product from Coconut Processing: Histomorphometry Characteristics in the Digestive and Renal Systems

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