Histological study of organogenesis and embryogenesis inCyclamen persicum Mill. tissue cultures: Evidence for a single organogenetic pattern

Wicart, G.; Mouras, A.; Lutz, A

doi:10.1007/bf01288870

Cited by 48 publications

(31 citation statements)

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“…Two types of regeneration pattern, adventitious bud formation and adventitious embryo formation are usually observed in in vitro culture of somatic tissues including cyclamen (Wicart et al 1984); the former starts with multicellular tissue and the latter with a single cell (Carole et al1993;Matsumoto et al 1996). Accordingly, chimerism is expected to yield more frequently in the starting material consisting of more than one cell at the moment of mutagenic event.…”

Section: Discussionmentioning

confidence: 99%

Production of novel flower color mutants from the fragrant cyclamen (Cyclamen persicum^|^times;C. purpurascens) by ion-beam irradiation

Ishizaka

Kondo

Kameari

2012

Plant Biotechnology

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Abstracte fragrant cyclamens 'Uruwashi-no-kaori' (UR), 'Kaori-no-mai' (KM), 'Kokou-no-kaori' (KO) were bred from amphidiploids that were derived by chromosome doubling of the hybrid among Cyclamen persicum 'Strauss', 'Pure White' and fragrant wild species of C. purpurascens. Amphidiploid of GBCP was produced by chromosome doubling of the hybrid between C. persicum 'Golden Boy' and fragrant wild species of C. purpurascens. In order to create novel ower colors, mutation breeding with carbon-ion-beam irradiation was carried out using amphidiploids of UR, KM and KO as well as dihaploids of UR, KM and GBCP. Immature petals or etiolated petioles of the amphidiploids and dihaploids were irradiated, and plant tissue culture techniques were used to produce material for additional investigations. Furthermore, the stepwise improvement of ower color was achieved by irradiating 'ion3', which had been derived from dihaploid of UR by ion-beam irradiation. e mutants thus obtained were evaluated for breeding new cultivars of fragrant cyclamens. e tissues were then analyzed in terms of their avonoid pigment content and their related genes. Key words:Flower pigment, fragrant cyclamen, ion-beam, mutation, tissue culture.The genus Cyclamen, family Myrsinaceae, includes 22 species. All species of Cyclamen form a tuber and consistently propagate by seed, but never propagate by natural splitting (Grey-Wilson 2002). Ornamental cyclamen cultivars, highly popular pot plants in several countries, have been developed through the crossing of selected natural mutants of wild C. persicum (2n=2x=48). Flowers of C. persicum-derived cultivars may have a purple, pink, red, pale yellow or white 'slip', with an 'eye'; occasionally, the slip is pale yellow, pale pink or white and there is no 'eye'. 'Eye' refers to the bases of the petals colored deep purple and 'slip' refers to the other, non-eye parts of the petal, of various colors (Figure 1). Moreover, these flowers emit woody-or powdery-like scent, which has been expected to improve. In contrast, C. purpurascens (2n=2x=34) has small owers that consist of a purple slip and a deep purple eye, that emit a sweet fragrance, i.e. a rose-, hyacinth-or lily of the valley-like fragrance ( Figure 1A) (Grey-Wilson 2002;Ishizaka et al. 2002). The ornamental value of the cultivars of C. persicum would be enhanced by the introduction of fragrance properties of C. purpurascens.However, C. purpurascens has been neither improved as a major commercial cultivar nor used as breeding material.In earlier work, an allodiploid (2n=2x=41) was produced by the crossing of C. persicum cultivars (2n=2x=48) and C. purpurascens (2n=2x=34) through ovule culture techniques for rescuing abortive hybrid embryos, although all the allodiploids were sterile (Ishizaka and Uematsu 1995). In our subsequent study, however, fertile allotetraploids (amphidiploid, 2n=4x=82) were successfully produced by inducing chromosome doubling with in vitro colchicine treatment of the hybrid ovules. Consequently, two types of fertile amphidiploid plant...

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Section: Discussionmentioning

confidence: 99%

Production of novel flower color mutants from the fragrant cyclamen (Cyclamen persicum^|^times;C. purpurascens) by ion-beam irradiation

Ishizaka

Kondo

Kameari

2012

Plant Biotechnology

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“…Only a few reports on somatic embryogenesis on cyclamen are available (Fersing et al 1982;Wicart et al 1984).…”

Section: Introductionmentioning

confidence: 99%

The effect of explant material on somatic embryogenesis of Cyclamen persicum Mill

Kiviharju

Tuominen

Törmälä

1992

Plant Cell Tiss Organ Cult

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Somatic embryos of Cyclamen persicum Mill. could be produced through a callus phase from juvenile explant material including anthers, ovaries and zygotic embryos. The auxin 2,4-D (1.0-1.5 mg 1-1) and coconut milk (10% v/v) in MS medium were important factors for the induction of somatic embryogenesis. Somatic embryos germinated into plantlets in MS medium without growth regulators. The plants grew well in the greenhouse and flowered normally. The plants were phenotypically identical to the mother plants with a few exceptions.

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“…Although C. persicum somatic embryos have been reported (Kiviharju et al 1992;Otani and Shimada 1991;Takamura et al 1995;Takamura and Tanaka 1996;Wicart et al 1984), the frequency of plant regeneration from somatic embryos is low.…”

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confidence: 99%

Improvement of regeneration and transformation systems for Cyclamen persicum using somatic embryo culture

Terakawa

Yamamura

Murayama

2008

Plant Biotechnology

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Cyclamen persicum is one of the most important pot plants in the world. We developed an effective method for somatic embryo-mediated plant regeneration and genetic transformation using Agrobacterium, with somatic embryos as the source of plant material. Numerous somatic embryos were formed from the leaf segments of Cyclamen cv. "Fragrance Mini" 16 weeks after initiation on a 2,4-D-and kinetin-containing medium. The maximum regeneration frequency from the somatic embryos was achieved using a regeneration medium containing 0.1 mg l Ϫ1 benzylaminopurine, 0.01 mg l Ϫ1 a-naphthaleneacetic acid, and 0.2 mg l Ϫ1 gibberellin. After co-cultivating the somatic embryos with Agrobacterium strain LBA4404 (the plasmid vector pIG121Hm) that harbors genes for hygromycin phosphotransferase, neomycin phosphotransferase, and b-glucuronidase, hygromycin-resistant clones were selected on a medium containing 5 mg l Ϫ1 hygromycin. Hygromycin-resistant plantlets were regenerated from 14 hygromycin-resistant embryos. Using this transformation system, 74 independent plants were obtained from 1-ml packed cell volume, approximately 2,000 embryos.

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Histological study of organogenesis and embryogenesis inCyclamen persicum Mill. tissue cultures: Evidence for a single organogenetic pattern

Cited by 48 publications

References 10 publications

Production of novel flower color mutants from the fragrant cyclamen (Cyclamen persicum^|^times;C. purpurascens) by ion-beam irradiation

Production of novel flower color mutants from the fragrant cyclamen (Cyclamen persicum^|^times;C. purpurascens) by ion-beam irradiation

The effect of explant material on somatic embryogenesis of Cyclamen persicum Mill

Improvement of regeneration and transformation systems for Cyclamen persicum using somatic embryo culture

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