The effect of explant material on somatic embryogenesis of Cyclamen persicum Mill

Kiviharju, Elina; Tuominen, U.; Törmälä, Timo

doi:10.1007/bf00055516

Cited by 38 publications

(26 citation statements)

References 6 publications

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“…Besides reports on organogenesis (reviewed by Takamura & Miyajima, 1997;Dillen et al, 1996), efficient protocols for somatic embryogenesis have been published (Wicart et al, 1984;Fukui et al, 1988;Otani & Shimada, 1991;Kiviharju et al, 1992;Kreuger et al, 1995;Takamura et al, 1995;. While some protocols included seedling tissues as starting material (Kreuger et al, 1995;Takamura et al, 1995), we have concentrated on the method starting from somatic tissue in ovules .…”

Section: Introductionmentioning

confidence: 97%

Genotypic differences in callus formation and regeneration of somatic embryos in Cyclamen persicum Mill

Winkelmann

Serek

2005

Euphytica

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This study was performed in order to check the applicability of a protocol for somatic embryogenesis in Cyclamen persicum and to compare the in vitro response of cultivars belonging to different cyclamen growth types and flower colours obtained from two breeders. One hundred ovules per plant were prepared from 32 tested F 1 hybrid cultivars represented by five plants each. Callus induction monitored after 8 weeks varied between 42 and 85%. Variation occurred between replications and single plants of a cultivar. Consistency and colour of the developing calluses were diverse. Only in 1 cultivar out of the 32 tested no regeneration of somatic embryos was obtained. The regeneration rates varied from 0 to 65% among cultivars. In the group of midi-type cultivars the regeneration response was lower than that in mini and giganteum types. High regeneration rates were observed in cultivars with flamed flowers.

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Section: Introductionmentioning

confidence: 97%

Genotypic differences in callus formation and regeneration of somatic embryos in Cyclamen persicum Mill

Winkelmann

Serek

2005

Euphytica

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“…In cyclamen (Cyclamen persicum) somatic embryogenesis is an effective vegetative in vitro propagation method (Kiviharju et al 1992;Kreuger et al 1995;Schwenkel and Winkelmann 1998;Winkelmann et al 2000). Mass propagation using embryogenic suspension cultures was found to be even more effective if bioreactor culture was applied (Hohe et al 1999a(Hohe et al ,b, 2001.…”

Section: Introductionmentioning

confidence: 98%

Cryopreservation of embryogenic suspension cultures of Cyclamen persicum Mill.

2004

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We have developed a simple protocol for the cryopreservation of embryogenic suspension cultures of Cyclamen persicum. Embryogenic suspension cultures in the linear growth phase 7-10 days after subculture were used for cryopreservation. Of the different cryoprotectants tested during a 2-day pre-culture, 0.6 M sucrose resulted in the highest re-growth rates of 75%. An additional pre-treatment with 0.6 M sucrose and 10% DMSO (dimethylsulfoxide) for 1 h also positively affected re-growth. Microscopic studies on viability revealed that only few small embryogenic cells survived cryopreservation, while vacuolated single cells died. Experiments in which the duration of the pre-culture period--i.e. the length of time the embryogenic suspension cells were exposed to 0.6 M sucrose--was varied showed that 2-4 days was the most optimal exposure time to 0.6 M sucrose. Callus re-grown after cryopreservation showed growth rates similar to that of unfrozen callus and regenerated even higher numbers of somatic embryos than unfrozen callus.

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“…Since cyclamen cannot be propagated from cuttings, in vitro culture techniques have to be applied. Many genotypes can be multiplied efficiently via somatic embryogenesis (Wicart et al 1984;Otani and Shimada 1991;Kiviharju et al 1992;Kreuger et al 1995;Takamura et al 1995;Schwenkel and Winkelmann 1998;Winkelmann and Serek 2005).…”

Section: Introductionmentioning

confidence: 99%

Proteomic analyses of somatic and zygotic embryos of Cyclamen persicum Mill. reveal new insights into seed and germination physiology

Winkelmann

Heintz²,

Dorsselaer³

et al. 2006

Planta

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In the horticulturally important ornamental species Cyclamen persicum Mill., somatic embryogenesis is an efficient vegetative propagation method and the development of artificial seeds is an ultimate aim. This study aims at a systematic comparison of the proteomes of zygotic embryos, somatic embryos grown in liquid medium containing 30 or 60 g l(-1) sucrose, germinating embryos of both types and endosperm in order to obtain novel insights into seed and germination physiology. Using high resolution two-dimensional isoelectric focussing/sodium dodecylsulfate polyacrylamide gel electrophoresis (2D IEF/SDS PAGE), 74% of the proteins expressed in zygotic embryos were found in similar abundance in somatic embryos grown in 60 g l(-1) sucrose. Somatic embryos grown in 30 g l(-1) sucrose accumulated fewer protein species than those grown in 60 g l(-1). Selected proteins were identified following mass spectrometry (nano-LC-MS/MS). Four enzymes involved in glycolysis (UDP-glucose pyrophosphorylase, fructose bisphosphate aldolase, triosephosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase GAPDH) were specifically induced in somatic embryos. 11S globulin proteins identified by MS were present in high levels in somatic embryos, zygotic embryos and endosperm, whereas 7S globulins were detected mainly in endosperm and zygotic embryos. These are the first storage proteins identified in C. persicum. Xyloglucans are known to be another group of seed storage compounds in C. persicum. Interestingly, xyloglucan endotransglycosylases were found to be highly expressed in endosperm tissue. We discuss the physiological implications of these observations.

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The effect of explant material on somatic embryogenesis of Cyclamen persicum Mill

Cited by 38 publications

References 6 publications

Genotypic differences in callus formation and regeneration of somatic embryos in Cyclamen persicum Mill

Genotypic differences in callus formation and regeneration of somatic embryos in Cyclamen persicum Mill

Cryopreservation of embryogenic suspension cultures of Cyclamen persicum Mill.

Proteomic analyses of somatic and zygotic embryos of Cyclamen persicum Mill. reveal new insights into seed and germination physiology

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