Genotypic differences in callus formation and regeneration of somatic embryos in Cyclamen persicum Mill

Abstract

This study was performed in order to check the applicability of a protocol for somatic embryogenesis in Cyclamen persicum and to compare the in vitro response of cultivars belonging to different cyclamen growth types and flower colours obtained from two breeders. One hundred ovules per plant were prepared from 32 tested F 1 hybrid cultivars represented by five plants each. Callus induction monitored after 8 weeks varied between 42 and 85%. Variation occurred between replications and single plants of a cultivar. Consistency and colour of the developing calluses were diverse. Only in 1 cultivar out of the 32 tested no regeneration of somatic embryos was obtained. The regeneration rates varied from 0 to 65% among cultivars. In the group of midi-type cultivars the regeneration response was lower than that in mini and giganteum types. High regeneration rates were observed in cultivars with flamed flowers.

Enolases: storage compounds in seeds? Evidence from a proteomic comparison of zygotic and somatic embryos of Cyclamen persicum Mill.

“…Somatic embryogenesis has been shown to be an alternative propagation system in Cyclamen (Wicart et al 1984;Kiviharju et al 1992;Schwenkel and Winkelmann 1998). The production of embryogenic calluses and induction of embryogenesis is well established for cultivars (Winkelmann and Serek 2005) and wild species (Prange et al 2010a, b). However, subsequent processes leading to physiological disorders, asynchronous development and misshaping in a relevant portion of emerged embryos are still not fully understood and thus hardly controllable.…”

Proteomic analyses of somatic and zygotic embryos of Cyclamen persicum Mill. reveal new insights into seed and germination physiology

“…Since cyclamen cannot be propagated from cuttings, in vitro culture techniques have to be applied. Many genotypes can be multiplied efficiently via somatic embryogenesis (Wicart et al 1984;Otani and Shimada 1991;Kiviharju et al 1992;Kreuger et al 1995;Takamura et al 1995;Schwenkel and Winkelmann 1998;Winkelmann and Serek 2005).…”

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“…When the duration of the treatment was extended to 13 weeks, the longest shoot (1.50±0.12) was generated from the sample treated with relatively low concentration of AC (0.5 g/L) and the highest concentration (1.75 mg/L) of BAP ( Table 1). In our study, higher concentrations of hormone affected shoot formation possibly related to the physiology and the multiformity of plantlets and the synthesis of endogenous hormones in the plantlets, different endogenous hormones of explants may differ in their ability to react to exogenously applied plant growth regulators (Winkelmann and Serek, 2005). Higher concentrations of AC and BAP (2.5 g/L, 1.75 mg/L raised number of leaves (32.40±2.0 and 46.4±3.6) after 9 and 13 weeks respectively (Table 2).…”