Highly sensitive quantification of vancomycin in plasma samples using liquid chromatography–tandem mass spectrometry and oral bioavailability in rats

Shibata, Nobuhito; Ishida, M.; Prasad, Y. V. Rama; Gao, Weihua; Yoshikawa, Yukako; Takada, Kanji

doi:10.1016/s1570-0232(03)00068-0

Cited by 61 publications

(52 citation statements)

References 14 publications

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“…Indeed, several LC-MS/MS methods for the quantification of serum vancomycin concentrations have been described previously [7][8][9]. These methods, however, rely on internal standard compounds with are structurally not related to the target analyze (teicoplanin, atenolol).…”

Section: Introductionmentioning

confidence: 99%

Quantification of vancomycin in human serum by LC-MS/MS

König¹,

Kobold²,

Fink³

et al. 2013

Clinical Chemistry and Laboratory Medicine

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Background: The aim of our work was to develop and validate a reliable LC-MS/MS-based measurement procedure for the quantification of vancomycin in serum, to be applied in the context of efforts to standardize and harmonize therapeutic drug monitoring of this compound using routine assays. Methods: Sample preparation was based on protein precipitation followed by ultrafiltration. In order to minimize differential modulation of ionization by matrix constituents extended chromatographic separation was applied leading to a retention time of 9.8 min for the analyte. Measurement was done by HPLC-ESI-MS/MS. For internal standardization the derivative vancomycin-glycin (ISTD) prepared by chemical synthesis was used, HPLC conditions ensured coelution of ISTD with the analyte. Results: In a bi-center validation total CVs of < 4% were observed for quality control material ranging from 5.3 mg/L to 79.4 mg/L; accuracy was ± 4%. No relevant ion suppression was observed. Comparative measurement of aliquots from 70 samples at the two validation sites demonstrated close agreement. Conclusions: Employing a closely related homologue molecule for internal standardization and the use of MS/ MS following highly efficient sample pre-fractionation by HPLC, the method described here can be considered to offer the highest level of analytical reliability realized so far for the quantification of vancomycin in human serum. Thus, the method is suitable to be used in a comprehensive reference measurement system for vancomycin.

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Section: Introductionmentioning

confidence: 99%

Quantification of vancomycin in human serum by LC-MS/MS

König¹,

Kobold²,

Fink³

et al. 2013

Clinical Chemistry and Laboratory Medicine

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“…Generally, it was observed that the majority of the applied methods in the determination of vancomycin with HPLC use UV detectors, working in a wavelength range from 198 nm 59 to 282 nm 60 . Few works employ other kind of detectors, for example, Favetta et al 62 use an electrochemical detector and Shibata et al 65 employ mass spectrometry for the detection.…”

Section: Chromatographic Methodsmentioning

confidence: 99%

“…Shibata et al 65 described a novel high-performance liquid chromatography tandem mass spectrometry method for determination of vancomycin in serum and urine without treatment. After the addition of internal standard, serum and urine samples were directly injected into a HPLC system consisting of an extraction column and dual analytical columns.…”

Section: Chromatographic Methodsmentioning

confidence: 99%

Analytical methods for vancomycin determination in biological fluids and in pharmaceuticals

et al. 2007

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Recebido em 25/8/05; aceito em 7/4/06; publicado na web em 31/10/06Vancomycin is a glycopeptide antibiotic employed in the treatment of infections caused by certain methicillin-resistant staphylococci. It is indicated also for patients allergic to penicillin or when there is no response to penicillins or cephalosporins. The adequate vancomycin concentration levels in blood serum lies between 5 and 10 mg/L. Higher values are toxic, causing mainly nephrotoxicity and ototoxicity. Various analytical methods are described in the literature: spectrophotometric, immunologic, biologic and chromatographic methods. This paper reviews the main analytical methods for vancomycin determination in biological fluids and in pharmaceutical preparations.

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“…As seen in Table 1 LOQ of three ranges were 2, 40 and 10 μg/mL respectively [26], with accuracy (recovery) between 74.5% and 111% this was absolute for quantification study. The correlation coefficient calculation and the regression analysis were performed without any type of mathematical transformation [27][28][29][30][31].…”

Section: Linearity and Loqmentioning

confidence: 99%