A Simple and Modified Method Development of Vancomycin Using High Performance Liquid Chromatography

Chauhan, Meenakshi; Bhatt, Nidhi

doi:10.4172/2157-7064.1000296

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Cited by 3 publications

(1 citation statement)

References 25 publications

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“…An HPLC method was performed on Nucleosil 120 C18 5 µm column at 35 • C with mobile phase containing acetonitrile (A): water, pH 2.0 (B) and formic acid was used to adjust the pH of water at a flow rate of 1 mL/min [87] .…”

Section: Chromatographic Methodsmentioning

confidence: 99%

Analytical methods for the determination of certain antibiotics used in critically ill patients

Ibrahim

El-Adl

Baraka

et al. 2020

J Pharm Biopharm Res

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Section: Chromatographic Methodsmentioning

confidence: 99%

Analytical methods for the determination of certain antibiotics used in critically ill patients

Ibrahim

El-Adl

Baraka

et al. 2020

J Pharm Biopharm Res

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Vancomycin selective electrode based on molecularly imprinted polymer

Díaz-Villavicencio,

Baeza-Fonte,

Luaces-Alberto

et al. 2023

Chem. Pap.

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A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin

Lima

Seba

Gonçalves

et al. 2017

Journal of Chromatographic Science

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Therapeutic monitoring of the antibiotic vancomycin is important to achieve specific plasma concentration and prevent toxic effects. Several assays have been described for vancomycin determination in clinical practice, but high-performance liquid chromatography is still considered the gold standard for the quantification of vancomycin. In this study, we developed a new and rapid high-performance liquid chromatography method requiring 50 μL of plasma for the quantification of vancomycin. Acetonitrile was used for processing plasma by protein precipitation (1:2.5). Isocratic chromatographic analysis was carried out on a C18 silica-based (2.7 μm) column with the mobile phase containing 20 mM ammonium acetate/formic acid buffer (pH 4.0):methanol 88:12 (v/v). A diode array detector was used for UV detection at 240 nm. This method was validated according to the Brazilian Health Surveillance Agency legislation and International Conference on Harmonization guidelines. The measurement range was 1-100 μg/mL, analysis time was 8 min, and intermediate precision was <12%, supporting the present method as a fast, simple, and effective alternative for therapeutic monitoring of vancomycin.

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A Simple and Modified Method Development of Vancomycin Using High Performance Liquid Chromatography

Cited by 3 publications

References 25 publications

Analytical methods for the determination of certain antibiotics used in critically ill patients

Analytical methods for the determination of certain antibiotics used in critically ill patients

Vancomycin selective electrode based on molecularly imprinted polymer

A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin

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