A Rapid and Simple HPLC Method for Therapeutic Monitoring of Vancomycin

Abstract

Therapeutic monitoring of the antibiotic vancomycin is important to achieve specific plasma concentration and prevent toxic effects. Several assays have been described for vancomycin determination in clinical practice, but high-performance liquid chromatography is still considered the gold standard for the quantification of vancomycin. In this study, we developed a new and rapid high-performance liquid chromatography method requiring 50 μL of plasma for the quantification of vancomycin. Acetonitrile was used for processing plasma by protein precipitation (1:2.5). Isocratic chromatographic analysis was carried out on a C18 silica-based (2.7 μm) column with the mobile phase containing 20 mM ammonium acetate/formic acid buffer (pH 4.0):methanol 88:12 (v/v). A diode array detector was used for UV detection at 240 nm. This method was validated according to the Brazilian Health Surveillance Agency legislation and International Conference on Harmonization guidelines. The measurement range was 1-100 μg/mL, analysis time was 8 min, and intermediate precision was <12%, supporting the present method as a fast, simple, and effective alternative for therapeutic monitoring of vancomycin.

Utility of Silver-nanoparticles for Nano-fluorimetric Determination of Vancomycin Hydrochloride in Pharmaceutical Formulation and Biological Fluids: Greenness Assessment

“…According to the literature survey, some techniques were reported for the estimation of VANH in different matrices including spectrophotometric [10][11][12], spectrofluorimetric [13][14][15], electrochemical [7,16,17], HPLC [18][19][20][21][22][23][24][25][26], LC-MS/MS [8,[27][28][29][30][31][32][33][34][35][36][37], capillary electrophoresis [38][39][40][41], radioimmunoassay and ELISA-based immunoassay techniques [42][43][44]. VANH has native fluorescence at 335 nm after excitation at 268 nm according to the previous study [14].…”

A New and Ecological Method to Quantify Vancomycin in Pharmaceutical Product by Infrared Spectrometry

“…[5][6][7] In the context of the importance of vancomycin in the drug scenario, the development of analytical methods for its evaluation becomes extremely fundamental. Some analytical methods for this drug both physico-chemical [8][9][10][11][12][13][14] and microbiological were found in literature. [15][16][17][18] The spectrophotometry is a kind of very useful analytical method, which provides precise results and can be applied to quantify drugs.…”

A simple and validated HPLC method for vancomycin assay in plasma samples: the necessity of TDM center development in Southern Iran

“…Although immunoassay methods because of the simplicity and fast evaluation process have been used frequently in many previous studies these methods due to the lack of enough precision and sensitivity and also due to the cross-reaction with vancomycin metabolites has its own limitations. In spite of the fact that HPLC is the most selective and sensitive method for vancomycin analysis in serum samples especially at lower concentrations ( 8 9 ), but analysis of vancomycin in biological matrices using HPLC method has been encountered many challenges that should be overcome.…”

“…The selected IS should have similar λ max to vancomycin in order to obtain the possibility of simultaneous analysis. Zidovudine ( 9 ), caffeine ( 11 ), cefuroxime ( 13 ), erythromycin ( 14 ), para amino benzoic acid ( 15 ), phenacetin, and norvancomycin ( 16 ) are the examples of IS which have been considered for vancomycin assay in plasma samples.…”

“…The other parameter which could highly affect the precision and efficiency of the HPLC method for vancomycin assay is the instrument condition such as the type of HPLC column, column temperature, column length, and internal diameter, lambda max (λ max ) and wavelength, mobile phase, flow rate, etc. ( 8 9 12 17 ). In this study, we worked on different variables to optimize the influencing parameters to propose a simple, low-cost, and validated HPLC method for the determination of vancomycin in blood samples.…”