Analytical methods for vancomycin determination in biological fluids and in pharmaceuticals

Vila, Marta Maria Duarte Carvalho; Oliveira, Robson Machado de; Gonçalves, Marcos Moisés; Tubino, Matthieu

doi:10.1590/s0100-40422007000200029

Cited by 32 publications

(19 citation statements)

References 44 publications

(59 reference statements)

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“…For vancomycin determination in human liquids, two types of analytical methods are mainly used: immunoassays (including fluorescence polarization immunoassay, RIA, and enzyme‐multiplied immunoassay) and LC techniques. In therapeutic drug monitoring practice, primarily immunochemical methods are applied owing to their simplicity, rapidity and the commercial availability of kits or automated analyzers. However, the immunochemical methods have several disadvantages, such as relatively high cost and the possibility of cross‐reactivity with other vancomycin‐related substances (vancomycin degradation products or compounds formed during the production process) .…”

Section: Determination Of Vancomycinmentioning

confidence: 99%

“…To the best of our knowledge, two reviews focused on vancomycin determination have been published in 2000 and 2007 . But in comparison with this work, the overviews and discussions of the LC and sample preparation methods for vancomycin measurement in biological samples were not so detailed and covered only the methods published until 2005.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Modern methods for vancomycin determination in biological fluids by methods based on high‐performance liquid chromatography – A review

Javorská

Krcmova

Solichová

et al. 2015

J of Separation Science

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Vancomycin is a glycopeptide antibiotic used in the therapy of severe bacterial infection. The monitoring of vancomycin levels is recommended because of its narrow therapeutic index and toxicity. This measurement is especially appropriate in patients with unstable renal functions, who receive high doses of vancomycin or present serious bacterial infections accompanied by important sequestration of liquids when it could be difficult to achieve the optimal therapeutic dose. Most of the methods for vancomycin determination in routine practice are immunoassays. However, chromatography-based techniques in combination with UV or mass spectrometry detection provide results with greater accuracy and precision also in complicated biological matrices. This review provides a detailed overview of modern approaches for the chromatographic separation of vancomycin in various biological samples and useful sample preparation procedures for vancomycin determination in various biological fluids.

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Section: Determination Of Vancomycinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modern methods for vancomycin determination in biological fluids by methods based on high‐performance liquid chromatography – A review

Javorská

Krcmova

Solichová

et al. 2015

J of Separation Science

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“…Winooski, VT, USA) [72]. Concentration of amikacin in eluates was determined by reacting 10-fold diluted samples with a mixture of acetylacetone and formaldehyde in a buffer solution of boric acid, acetic acid, and phosphoric acid at pH 2.7 and boiling for 20 minutes.…”

Section: Elution and Eluate Activity Studiesmentioning

confidence: 99%

Novel Antibiotic-loaded Point-of-care Implant Coating Inhibits Biofilm

Jennings

Carpenter

Troxel

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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Background Orthopaedic biomaterials are susceptible to biofilm formation. A novel lipid-based material has been developed that may be loaded with antibiotics and applied as an implant coating at point of care. However, this material has not been evaluated for antibiotic elution, biofilm inhibition, or in vivo efficacy.Questions/purposes (1) Do antibiotic-loaded coatings inhibit biofilm formation? (2) Is the coating effective in preventing biofilm in vivo? Methods Purified phosphatidylcholine was mixed with 25% amikacin or vancomycin or a combination of 12.5% of both. A 7-day elution study for coated titanium and stainless steel coupons was followed by turbidity and zone of inhibition assays against Staphylococcus aureus and Pseudomonas aeruginosa. Coupons were inoculated with bacteria and incubated 24 hours (N = 4 for each test group). Microscopic images of biofilm were obtained. After washing and vortexing, attached bacteria were counted. A mouse biofilm model was modified to include coated and uncoated stainless steel wires inserted into the lumens of catheters inoculated with a mixture of S aureus or P aeruginosa. Colony-forming unit counts (N = 10) and scanning electron microscopy imaging of implants were used to determine antimicrobial activity. Results Active antibiotics with colony inhibition effects were eluted for up to 6 days. Antibiotic-loaded coatings inhibited biofilm formation on in vitro coupons (log-fold reductions of 4.3 ± 0.4 in S aureus and 3.1 ± 0 for P aeruginosa in phosphatidylcholine-only coatings, 5.6 ± 0 for S aureus and 3.1 ± 0 for P aeruginosa for combinationloaded coatings, 5.5 ± 0.3 for S aureus in vancomycinloaded coatings, and 3.1 ± 0 for P aeruginosa for amikacinloaded coatings (p \ 0.001 for all comparisons of antibiotic-loaded coatings against uncoated controls for both bacterial strains, p \ 0.001 for comparison of antibioticloaded coatings against phosphatidylcholine only for S aureus, p = 0.54 for comparison of vancomycin versus combination coating in S aureus, P = 0.99 for comparison of antibiotic-and unloaded phosphatidylcholine coatings in P aeruginosa). Similarly, antibiotic-loaded coatings reduced attachment of bacteria to wires in vivo (log-fold

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“…In the clinical laboratory the use of this technique has been efficient for determination of analytes in several biological fluids. Therefore, HPLC has been developed for the analysis of vancomycin in medicines, as well as for the determination in biological fluids 17,24,[26][27][28][29][30][31][32][33][34] . Kitahashi and Furuta 35 developed an assay for measuring vancomycin concentration by micellar electrokinetic capillary chromatography with detection at 210 nm using direct serum injection.…”

Section: Clinical Applicationmentioning

confidence: 99%

Untitled

Tariq¹,

Siddiqui²,

Kumar³

et al. 2010

ScienceAsia

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A reverse phase-liquid chromatographic method with UV detection at 280 nm is described for simultaneous determination of ceftriaxone sodium and vancomycin hydrochloride. Chromatographic separation of the two drugs was achieved on a Betasil C-1 column using a mobile phase consisting of a binary mixture of acetonitrile and triethylamine buffer adjusted to pH 3.5 ± 0.1 with orthophosphoric acid in a ratio of 20:80. The liquid chromatographic method developed offers symmetric peak shape, good resolution, and reasonable retention time for both drugs. Linearity, accuracy, and precision were found to be acceptable over the concentration ranges 125-750 ppm for ceftriaxone and 62.5-375 ppm for vancomycin. The liquid chromatographic method was successfully applied to the quality control of formulated products, plasma, and cerebrospinal fluid samples containing ceftriaxone and vancomycin.

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Analytical methods for vancomycin determination in biological fluids and in pharmaceuticals

Cited by 32 publications

References 44 publications

Modern methods for vancomycin determination in biological fluids by methods based on high‐performance liquid chromatography – A review

Modern methods for vancomycin determination in biological fluids by methods based on high‐performance liquid chromatography – A review

Novel Antibiotic-loaded Point-of-care Implant Coating Inhibits Biofilm

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