Highly informative multiclass profiling of lipids by ultra-high performance liquid chromatography – Low resolution (quadrupole) mass spectrometry by using electrospray ionization and atmospheric pressure chemical ionization interfaces

Beccaria, Marco; Inferrera, Veronica; Rigano, Francesca; Goryński, Krzysztof; Purcaro, Giorgia; Pawliszyn, Janusz; Dugo, Paola; Mondello, Luigi

doi:10.1016/j.chroma.2017.06.017

Cited by 18 publications

(14 citation statements)

References 34 publications

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“…However, LC-MS has been rarely used but its importance for fatty acid elucidation is increasing [15][16][17][18][19][20][21]. Typically, these methods use a targeted multiple reaction monitoring (MRM) approach [22]. The disadvantage of MRM is a limited number of targets and low mass resolution because of the typically used triple quadruple and linear ion trap mass spectrometers [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Since unmodified fatty acids show no or only little fragmentation in MS/MS experiments, a high mass resolution is crucial for lipid identification based on precursor mass accuracy. Increasingly, fatty acids are detected next to other lipid species in an untargeted manner during lipidomics analysis . Unfortunately, free fatty acids are only present in relative low concentrations next to high abundant lipid species like phospholipids and glycerols.…”

Section: Introductionmentioning

confidence: 99%

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Free fatty acid profiling in marine algae extract by LC‐MS/MS and isolation as well as quantification of the ω‐3 fatty acid hexadeca‐4,7,10,13‐tetraenoic acid

Schlotterbeck

Kolb

Lämmerhofer

2018

J of Separation Science

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Undaria pinnatifida (Wakame) alga contains high amounts of hexadeca‐4Z,7Z,10Z,13Z‐tetraenoic acid which was reported to decrease the efficiency of cisplatin chemotherapeutics. To obtain a fatty acid enriched extract of this ω‐3 poly‐unsaturated fatty acid as an analytical standard, Wakame was used as source material for its extraction. A two‐step extraction protocol consisting of a liquid‐liquid extraction followed by solid‐phase extraction with 3‐aminopropyl silica in accordance to a normal‐phase elution mode was developed. An ultra high performance liquid chromatography with electrospray ionization tandem mass spectrometry method based on sequential windowed acquisition of all theoretical fragment ion mass spectra allowed a simultaneous comprehensive group selective fatty acids profiling in untargeted manner and quantitative analysis of the targeted fatty acid. Hexadeca‐4Z,7Z,10Z,13Z‐tetraenoic acid was identified using high‐resolution product ion spectra. The quantitative method was based on d5‐deuterated hexadeca‐4Z,7Z,10Z,13Z‐tetraenoic acid which was employed as surrogate calibrant. Preliminary method validation was performed by evaluating detection and quantification limits, linear range, intra‐assay and inter‐day precision. Finally, a concentration of 421.2 ± 14.9 ng/mL (4% CV) of hexadeca‐4Z,7Z,10Z,13Z‐tetraenoic acid was determined in the extract which was further used as analytical standard.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Free fatty acid profiling in marine algae extract by LC‐MS/MS and isolation as well as quantification of the ω‐3 fatty acid hexadeca‐4,7,10,13‐tetraenoic acid

Schlotterbeck

Kolb

Lämmerhofer

2018

J of Separation Science

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“…Finally, as is already well known, saturated FAs do not occupy the sn-2 position in vegetable oils; thus, palmitic (P, C16:0) and stearic acids (S, C18:0) most probably are in the sn-1/sn-3 positions of the OOP and SOL species, as highlighted by the APCI–MS spectra. To this regard, it is worth remembering that APCI source, particularly suitable for the ionization of non-polar compounds, produces some in-source fragmentation, thus generating some diagnostic fragments [ 36 ], useful to achieve a reliable identification, without the need for MS/MS experiments, as in the present work, in which a single quadrupole MS system was employed.…”

Section: Resultsmentioning

confidence: 99%

Identification of Fatty Acid, Lipid and Polyphenol Compounds from Prunus armeniaca L. Kernel Extracts

Hrichi

Rigano

Chaâbane-Banaoues

et al. 2020

Foods

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Apart from its essential oil, Prunus armeniaca L. kernel extract has received only scarce attention. The present study aimed to describe the lipid and polyphenolic composition of the dichloromethane, chloroform, ethyl acetate, and ethanol extracts on the basis of hot extraction, performing analysis by gas chromatography and high-performance liquid chromatography coupled with mass spectrometry. A total of 6 diacylglycerols (DAGs) and 18 triacylglycerols (TAGs) were detected as being present in all extracts, with the predominance of OLL (dilinoleyl-olein), OOL (dioleoyl-linolein), and OOO (triolein), with percentages ranging from 19.0–32.8%, 20.3–23.6%, and 12.1–20.1%, respectively. In further detail, the extraction with ethyl acetate (medium polarity solvent) gave the highest signal for all peaks, followed by chloroform and dichloromethane (more apolar solvent), while the extraction with ethanol (polar solvent) was the least efficient. Ethanol showed very poor signal for the most saturated TAGs, while dichloromethane showed the lowest percentages of DAGs. Accordingly, the screening of the total fatty acid composition revealed the lowest percentage of linoleic acid (C18:2n6) in the dichloromethane extract, which instead contained the highest amount (greater than 60%) of oleic acid (C18:1n9). Polyphenolic compounds with pharmacological effects (anti-tumor, anti-coagulant, and inflammatory), such as coumarin derivative and amygdalin, occurred at a higher amount in ethyl acetate and ethanol extracts.

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“…HPLC-MS analysis has been implemented in recent years to simplify sample preparation. The use of MS/MS has enabled MS structural elucidation of individual triacylglycerols [37,38].…”

Section: Lipidsmentioning

confidence: 99%

Analytical Strategies for Fingerprinting of Antioxidants, Nutritional Substances, and Bioactive Compounds in Foodstuffs Based on High Performance Liquid Chromatography–Mass Spectrometry: An Overview

Donno

Mellano

Gamba

et al. 2020

Foods

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New technology development and globalisation have led to extreme changes in the agri-food sector in recent years that need an important food supply chain characterisation from plant materials to commercial productions. Many analytical strategies are commonly utilised in the agri-food industry, often using complementary technologies with different purposes. Chromatography on-line coupled to mass spectrometry (MS) is one of the most selective and sensitive analytical methodologies. The purpose of this overview is to present the most recent MS-based techniques applied to food analysis. An entire section is dedicated to the recent applications of high-resolution MS. Covered topics include liquid (LC)– and gas chromatography (GC)–MS analysis of natural bioactive substances, including carbohydrates, flavonoids and related compounds, lipids, phenolic compounds, vitamins, and other different molecules in foodstuffs from the perspectives of food composition, food authenticity and food adulteration. The results represent an important contribution to the utilisation of GC–MS and LC–MS in the field of natural bioactive compound identification and quantification.

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Highly informative multiclass profiling of lipids by ultra-high performance liquid chromatography – Low resolution (quadrupole) mass spectrometry by using electrospray ionization and atmospheric pressure chemical ionization interfaces

Cited by 18 publications

References 34 publications

Free fatty acid profiling in marine algae extract by LC‐MS/MS and isolation as well as quantification of the ω‐3 fatty acid hexadeca‐4,7,10,13‐tetraenoic acid

Free fatty acid profiling in marine algae extract by LC‐MS/MS and isolation as well as quantification of the ω‐3 fatty acid hexadeca‐4,7,10,13‐tetraenoic acid

Identification of Fatty Acid, Lipid and Polyphenol Compounds from Prunus armeniaca L. Kernel Extracts

Analytical Strategies for Fingerprinting of Antioxidants, Nutritional Substances, and Bioactive Compounds in Foodstuffs Based on High Performance Liquid Chromatography–Mass Spectrometry: An Overview

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