Analytical Strategies for Fingerprinting of Antioxidants, Nutritional Substances, and Bioactive Compounds in Foodstuffs Based on High Performance Liquid Chromatography–Mass Spectrometry: An Overview

Abstract

New technology development and globalisation have led to extreme changes in the agri-food sector in recent years that need an important food supply chain characterisation from plant materials to commercial productions. Many analytical strategies are commonly utilised in the agri-food industry, often using complementary technologies with different purposes. Chromatography on-line coupled to mass spectrometry (MS) is one of the most selective and sensitive analytical methodologies. The purpose of this overview is to present the most recent MS-based techniques applied to food analysis. An entire section is dedicated to the recent applications of high-resolution MS. Covered topics include liquid (LC)– and gas chromatography (GC)–MS analysis of natural bioactive substances, including carbohydrates, flavonoids and related compounds, lipids, phenolic compounds, vitamins, and other different molecules in foodstuffs from the perspectives of food composition, food authenticity and food adulteration. The results represent an important contribution to the utilisation of GC–MS and LC–MS in the field of natural bioactive compound identification and quantification.

Quantitative and Comparative Investigation of Plasmalogen Species in Daily Foodstuffs

“…With regards to plasmalogen, although it has been reported to exist in animals, such as livestock, poultry, and seafood [ 10 , 17 ], the quantitative comparation in different animal foodstuffs have not yet been well studied. To date, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been widely applied in the analysis of food products, such as bioactive constituent discovery, food authentication and discrimination, quality control, and so on [ 16 , 18 , 19 , 20 ]. For the source exploration of plasmalogen, most of the studies focused on ethanolamine plasmalogen (PlsEtn) [ 10 , 21 ], while the other major type, choline plasmalogen (PlsCho), attracted less attention.…”

Vitis vinifera L. Pruning Waste for Bud-Preparations as Source of Phenolic Compounds–Traditional and Innovative Extraction Techniques to Produce New Natural Products

“…The scientific literature on analytical strategies describes UV-Visible/Fluorescence spectroscopy and HPLC fingerprint as the most important characterization tools in natural product analysis [29][30][31]. This study showed a preliminary polyphenolic pattern of V. vinifera bud-macerates, commercial bud-preparations, and ultrasound extracts to compare bud-derivatives obtained with different extraction methods (maceration and ultrasounds).…”

Screening, Identification, and Quantification of Nutritional Components and Phytochemicals in Foodstuffs

“…Donno et al [ 11 ] investigated the main analytical strategies commonly utilized in the agri-food industry, often using complementary technologies with different purposes. This overview presented the most recent MS-based techniques applied to food analysis.…”