Dried fruits are important, healthy and popular snacks, despite the limited information on their nutritional profiles and phytochemical composition. The present work was aimed to study the chemical composition of freeze-dried fruits from four fruit species: two common commercial snacks (apple and goji) and two innovative products (kaki and kiwi). Sugar and organic acid levels, total phenolics (TPC), and main health-promoting phytochemicals were studied by HPLC fingerprinting analysis. Furthermore, in vitro antioxidant capacity (AOC) was preliminarily observed in these products. A Principal Component Analysis (PCA) was carried out as a multivariate approach as well. The TPC ranged from 210.9 mg GAE/100g DW (kiwi) to 872.6 mg GAE/100g DW (kaki), while dried fruit antioxidant capacity ranged from 23.09 mmol Fe2+/kg DW (goji) to 137.5 mmol Fe2+/kg DW (kaki). The most important phytochemical class in apple (two cultivars), kiwi, and kaki dried fruits was phenolics (from 74.6% to 93.3%), while monoterpenes were the first class in goji (67.5%). No anthocyanins have been identified in dried fruits because these compounds are most likely converted to phenolic acids during the drying process. This research intended to stimulate large-scale exploitation of commercial dried fruits as functional foods as well.
A large species diversity characterises the wide distribution of chestnuts in Asia, North America, and Europe, hence reflecting not only the adaptation of the genus Castanea to diverse environmental conditions, but also to different management strategies encompassing orchards. The characterisation and description of chestnut populations and cultivars are crucial to develop effective conservation strategies of one of the most important Italian and European fruit and wood species. Chestnut cultivars grown in the same pedoclimatic conditions and on the same clonal rootstock were characterised with sensory, spectrophotometric, and chromatographic analysis to determine the phytochemical composition and nutraceutical properties. A multivariate approach, including principal component analysis and conditional inference tree models, was also performed. The ease of peeling, seed colour, and intensity of sweetness were the sensory descriptors that allowed us to differentiate C. sativa cultivars. Antioxidant capacity ranged from 9.30 ± 0.39 mmol Fe+2 kg−1 DW (‘Bouche de Bètizac’) to 19.96 ± 1.89 mmol Fe+2 kg−1 DW (‘Garrone Rosso’). Monoterpenes represented the main component, reaching 88% for hybrids, followed by polyphenols (10–25% for hybrids and chestnuts, respectively). A multivariate approach showed that phenolic acids and tannins were the bioactive classes with the highest discriminating power among different genotypes, and that genotype is a significant variable (p < 0.05). In addition, most of the analysed chestnut cultivars showed a content of bioactive compounds similar to or higher than the main hazelnut, walnut, and almond varieties. Chestnut agrobiodiversity could be intended as strictly associated to the genotype effect and underlines the large variability within the genus Castanea, and therefore, the importance of in farm and ex situ conservation of local germplasm is part of a global strategy aimed at increasing the levels of agrobiodiversity.
Autumn olive (Elaeagnus umbellata Thunb.) is a deciduous shrub tree widely distributed in Asia and Southern Europe and grown as ornamental species. It is locally used for human consumption, as relevant medical value is attributed to the berries. Information about its composition, especially concerning the characterization of bioactive and health-promoting compounds, is limited. The aim of the present study is to evaluate the main bioactive compounds and nutraceutical proprieties of autumn olive fruits, via high-performance liquid chromatography (HPLC) fingerprint and spectrophotometric analysis, in order to strengthen the knowledge about this underrated species and promote potential applications as a food supplement. Concerning nutraceutical traits, total polyphenolic content (325.366 ± 13.019 mg of gallic acid equivalents (mg GAE)/100 of fresh weight (g FW)) and total anthocyanin content (194.992 ± 0.817 mg of cyanidin-3-O-glucoside (mg C3G)/100 g FW) recorded considerable values. The phytochemical fingerprint revealed the presence 23 bioactive compounds. Polyphenols (65.56%) were the largest class, followed by monoterpenes (27.40%) and vitamin C (7.04%). Anthocyanins were the most represented compounds among polyphenols (71.9%). The antioxidant capacity (20.031 ± 1.214 mmol Fe2+/kg) was similar to that recorded for other small fruits with proven health-promoting properties. The present work underlined the potential of E. umbellata as a source of health-promoting bioactive compounds. Further studies should deepen the knowledge of nutraceutical aspects, which turned out to be interesting.
New technology development and globalisation have led to extreme changes in the agri-food sector in recent years that need an important food supply chain characterisation from plant materials to commercial productions. Many analytical strategies are commonly utilised in the agri-food industry, often using complementary technologies with different purposes. Chromatography on-line coupled to mass spectrometry (MS) is one of the most selective and sensitive analytical methodologies. The purpose of this overview is to present the most recent MS-based techniques applied to food analysis. An entire section is dedicated to the recent applications of high-resolution MS. Covered topics include liquid (LC)– and gas chromatography (GC)–MS analysis of natural bioactive substances, including carbohydrates, flavonoids and related compounds, lipids, phenolic compounds, vitamins, and other different molecules in foodstuffs from the perspectives of food composition, food authenticity and food adulteration. The results represent an important contribution to the utilisation of GC–MS and LC–MS in the field of natural bioactive compound identification and quantification.
The FINNOVER project provides a technical and economic path for the creation of new supply chains, or their development, for the sustainable extraction and utilization of natural biologically active molecules. In this research, a green extraction method and re-use strategy of botanical by-products are developed and used to obtain value-added products as an ecological alternative to waste composting or incineration. It was applied to the production of Castanea spp. bud-derivatives (a specific category of food supplements derived from plant material) and their by-products. This study aimed to compare the phytochemical composition of Castanea spp. bud-preparations (herbal preparations from buds and sprouts) with the extracts derived from the bud-waste management process. Bioactive compounds were extracted by maceration in hydroglyceroalcoholic solution (traditional method) and by a green extraction technique (Pulsed Ultrasound-Assisted Extraction—PUAE) and identified and quantified by HPLC methods obtaining a specific chromatographic profile. PUAE extracted a total content of 160.42 mg/100 g FW, a good amount if compared to the relative commercial product (1276.17 mg/100 g FW). About 13% of the chemical content of Castanea spp. bud-preparations was maintained in the by-product extracts. This research showed that PUAE may be considered as a sustainable alternative to waste management based on incineration or composting. This study could be considered by the industries for the development of new products for the market derived from the bud-derivative processing waste.
In the Comoros Islands, as in other developing countries, malnutrition and food insecurity affect a very large percentage of the population. Developing fruit-based products in order to make profit, reduce poverty and improve indigenous people diet could be very important for local population of countries as Comoros Islands. The aim of the present work was to study the chemical composition of jams and jellies produced from seven fruit species harvested in Grand Comore Island. The following parameters were studied sugars and organic acids, total phenolics, total anthocyanins and high-performance liquid chromatography (HPLC) fingerprint of the main phytochemicals. Antioxidant activity was also measured. A multivariate approach (Principal Component Analysis) was performed in order to better characterize the products and to set a potential analytical tool for jam characterisation. Results showed that the analysed products are a good source of polyphenolic constituents, as caffeic and gallic acids, catechin and quercetin and volatile compounds, as limonene and γ-terpinene: these molecules may be considered as suitable markers for these fruit-derived products as characterizing the chromatographic patterns. The characterisation of these products and their nutritional and nutraceutical traits is important as valorisation of local food production for poverty reduction and rural development. Further benefits of this approach include the maintenance of local agro-biodiversity as raw material for fruit-based products and the strengthening of food security practices.
Although modern medicine is available in many developing countries, such as the Comoros Islands, the primary health-care needs of the local population are based on traditional foods and beverages derived from natural resources and medicinal plants for cultural and historical reasons. Aphloia theiformis (Vahl) Benn. (‘Mfandrabo’), Cinnamomum verum J.Presl (‘Mani yamdrara’), Ocimum gratissimum L. (‘Roulé’), Plectranthus amboinicus (Lour.) Spreng. (‘Ynadombwe’), Cymbopogon nardus (L.) Rendle (‘Sandze monach’) and Ocimum americanum L. (‘Kandza’) are six wild plants that are largely utilised to treat many diseases. The leaves of these plants are used in the traditional Comorian tea (aqueous infusion). This study aimed to identify and quantify the main health-promoting compounds in the traditional formulation of Comorian tea by HPLC profiling together with a preliminary assessment of antioxidant capacity to confirm the traditional use of these plants by the local population. The single plants were also studied. The Comoros tea presented a total polyphenolic content (TPC) of 4511.50 ± 74.41 mgGAE/100 g DW, a value higher than the TPCs of the different plants included in the Comorian tea. Moreover, the Comorian tea showed an antioxidant capacity (AOC) of 578.65 ± 6.48 mmol Fe2+/Kg DW, a value higher if compared to all the AOC values obtained in the single plants. The polyphenolic fraction (771.37 ± 35.76 mg/100 g DW) and organic acids (981.40 ± 38.38 mg/100 g DW) were the most important phytochemical classes in the Comorian tea (40.68% and 51.75% of the total phytocomplex, respectively), followed by the monoterpenes (5.88%) and vitamin C (1.67%), while carotenoids were detected in trace (0.02%). The Comorian tea could be important in meeting the high demand in the Comoros Islands and other developing countries for cost-effective and natural health-promoting foods and/or beverages to be produced by agri-food industries and used by the local population. This study may promote traditional foods in rural communities in the Comoros Islands and contribute to sustainable rural development and a commercial valorisation of these plants for health-promoting and food applications.
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