High-sensitive and multiplex biosensing assay of NSCLC-derived exosomes via different recognition sites based on SPRi array

Fan, Yunpeng; Duan, Xiaolu; Zhao, Min; Wei, Xiaotong; Wu, Jiangling; Chen, Wenqin; Liu, Ping; Cheng, Wei; Cheng, Quan; Ding, Shijia

doi:10.1016/j.bios.2020.112066

Cited by 68 publications

(51 citation statements)

References 36 publications

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“…On the one hand, a single protein can be used to achieve the recognition and capture of exosomes; on the other hand, multiple specific proteins can also be used for multiple recognition and determination. Fan et al [54] established a SPRi biosensors through the different recognition sites and biological affinity of antibodies to multiple identify exosomes derived from non-small cell lung cancer(NSCLC) with high sensitivity. Thus, accurate identification of exosomes protein patterns can differentiate exosomes effectively from normal lung and NSCLC cells.…”

Section: Protein Recognition Based On Immunoreactionmentioning

confidence: 99%

Recent Progress on the Isolation and Detection Methods of Exosomes

Jiang

Liu

2020

Chemistry An Asian Journal

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Exosomes are known as one of extracellular vesicles, which are found in various body fluids and released by cells. As transport carrier, exosomes participate actively in intercellular communication and reflect their characteristics uniquely to the origin cells. Due to their unique biological physical properties and physiological functions, exosomes are considered to be one of best biomarkers of cancer diagnosis. At the same time, exosomes are potential therapeutic targets and drug delivery carriers. Therefore, the characteristics, functions and analytical methods of exosomes have increasingly attracted wide attention among scientists. In this review, the recent research progress on the basic characteristics and functional applications of exosomes are summarized. Furthermore and importantly, this review focuses on the recent advance in the purification and test methods of exosomes in recent years. Finally, issues pertaining to exosome detection are presented. Based on newly discovered characteristic of exosomes, the opportunities and challenges for future research of the purification and quantitative detection methods are outlined.

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Section: Protein Recognition Based On Immunoreactionmentioning

confidence: 99%

Recent Progress on the Isolation and Detection Methods of Exosomes

Jiang

Liu

2020

Chemistry An Asian Journal

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“…The same SPRi‐based sensing concept has been recently used to develop a biosensing assay for the highly sensitive and multiplex characterization of exosomes from nonsmall cell lung cancer, with an LoD of 10 000 exosomes per µL. [ 161 ] This LoD value was achieved by enhancing the assay using antibody‐functionalized AuNPs.…”

Section: Sensing Platforms Based On Plasmon Resonance and Surface‐enhmentioning

confidence: 99%

Photonic Technologies for Liquid Biopsies: Recent Advances and Open Research Challenges

Dell’Olio

Huser

et al. 2020

Laser & Photonics Reviews

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The recent development of sophisticated techniques capable of detecting extremely low concentrations of circulating tumor biomarkers in accessible body fluids, such as blood or urine, could contribute to a paradigm shift in cancer diagnosis and treatment. By applying such techniques, clinicians can carry out liquid biopsies, providing information on tumor presence, evolution, and response to therapy. The implementation of biosensing platforms for liquid biopsies is particularly complex because this application domain demands high selectivity/specificity and challenging limit‐of‐detection (LoD) values. The interest in photonics as an enabling technology for liquid biopsies is growing owing to the well‐known advantages of photonic biosensors over competing technologies in terms of compactness, immunity to external disturbance, and ultrahigh spatial resolution. Some encouraging experimental results in the field of photonic devices and systems for liquid biopsy have already been achieved by using fluorescent labels and label‐free techniques and by exploiting super‐resolution microscopy, surface plasmon resonance, surface‐enhanced Raman scattering, and whispering gallery mode resonators. The current state‐of‐the‐art is critically reviewed here, starting from the requirements imposed by the detection of the most common circulating biomarkers. Open research challenges are considered together with competing technologies, and the most promising paths of improvement are discussed for future applications.

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“…Methods for sensitive and specific identification of tumour-derived exosomes is being explored for NSCLC, not only for cancer detection but also for metabolic staging and predicting treatment outcomes. In recent work, Fan and co-workers [63] utilised a biosensor to determine populations of NSCLC-derived exosomes using antibody-functionalized GNPs. The strategy was able to distinguish successfully between different LC subtypes and was used to evaluate the therapeutic efficacy by measuring the concentration of exosomes in representative human plasma samples.…”

Section: Novel Sensing Approachesmentioning

confidence: 99%

Gold nanomaterials in the management of lung cancer

Perry

Wilton‐Ely

Tetley

2020

Emerging Topics in Life Sciences

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Lung cancer (LC) is one of the most deadly cancers worldwide, with very low survival rates, mainly due to poor management, which has barely changed in recent years. Nanomedicines, especially gold nanomaterials, with their unique and size-dependent properties offer a potential solution to many challenges in the field. The versatility afforded by the shape, size, charge and surface chemistry of gold nanostructures allows them to be adapted for many applications in the diagnosis, treatment and imaging of LC. In this review, a survey of the most recent advances in the field is presented with an emphasis on the optical properties of gold nanoscale materials and their use in cancer management. Gold nanoparticle toxicology has also been a focus of interest for many years but the studies have also sometimes arrived at contradictory conclusions. To enable extrapolation and facilitate the development of medicines based on gold nanomaterials, it must be assumed that each design will have its own unique characteristics that require evaluation before translation to the clinic. Advances in the understanding and recognition of the molecular signatures of LC have aided the development of personalised medicines. Tailoring the treatment to each case should, ideally increase the survival outcomes as well as reduce medical costs. This review seeks to present the potential of gold nanomaterials in LC management and to provide a unified view, which will be of interest to those in the field as well as researchers considering entering this highly important area of research.

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High-sensitive and multiplex biosensing assay of NSCLC-derived exosomes via different recognition sites based on SPRi array

Cited by 68 publications

References 36 publications

Recent Progress on the Isolation and Detection Methods of Exosomes

Recent Progress on the Isolation and Detection Methods of Exosomes

Photonic Technologies for Liquid Biopsies: Recent Advances and Open Research Challenges

Gold nanomaterials in the management of lung cancer

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