High resolution 1H NMR spectroscopic studies on dynamic biochemical processes in incubated human seminal fluid samples

Tomlins, Andrew M.; Foxall, P.J.D.; Lynch, Michael J.; Parkinson, John A.; Everett, Jeremy R.; Nicholson, Jeremy K.

doi:10.1016/s0304-4165(97)00116-5

Cited by 59 publications

(40 citation statements)

References 22 publications

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“…There has been growing interest in the field of metabolomics which provides useful information about the phenotype an organism through analysis of various biomarkers in body fluids [22,35,39,46,37,7]. This technology provides for assessment of metabolic changes within an organism, even when standard clinical chemistry markers are within normal limits [31].…”

Section: Introductionmentioning

confidence: 99%

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Jayaraman

Ghosh

Sengupta

et al. 2014

J Assist Reprod Genet

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Purpose The aim of this study was to analyze the seminal plasma of patients with idiopathic/male factor infertility and healthy controls with proven fertility by NMR spectroscopy, with a hope of establishing difference in biomarker profiles, if any, between the groups. Methods A total of 103 subjects visiting the infertility clinic of Manipal University with normozoospermic parameters, oligozoospermia, asthenozoospermia, azoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria. Seminal plasma was subjected to NMR spectroscopy at a 700 MHz

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Section: Introductionmentioning

confidence: 99%

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Jayaraman

Ghosh

Sengupta

et al. 2014

J Assist Reprod Genet

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“…The decay/growth curves for the phosphorylcholine and choline peak integrals, respectively, were reproducible when separate samples were measured at different temperatures (277 K/ 298 K) or with/without 1 mM tartrate using a simple two-factor experiment (Figure 3.2). The spectral profiles for ejaculate samples in our dataset were comparable to those reported previously in NMR studies of this biofluid 304 , and an NMR spectrum for a representative ejaculate sample is shown in Figure A1.1a.…”

Section: -Establishing Reproducibility Of Kinetic Curvessupporting

confidence: 85%

“…Indeed, the effects of tartrate and temperature on PAP enzyme activity were consistent across all three subjects, as would be expected when effectively performing a kinetic assay of the same enzyme in three subjects, and are thus expected to translate to samples from other patients. Finally, our study design and methods were based on a landmark study 304 , which used 3 patients, each providing 2 samples, to characterise the biochemical processes in human ejaculate. Our study used 13 samples and yielded equivalent results, whilst we also explored the role of tartrate and temperature inhibition of PAP in detail.…”

Section: Discussionmentioning

confidence: 99%

Multiple markers for the non-invasive diagnosis and characterisation of prostate cancer

Roberts¹

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The early detection of prostate cancer (CaP), the most common internal cancer in men, is limited by an absence of biomarkers that accurately reflect clinically significant disease.Currently, the prostate cancer antigen 3 (PCA3) test in combination with TMPRSS2-ERG fusion gene detection in urine obtained following rectal prostatic massage represent arguably the best standalone biomarkers for CaP but are not widely used in comparison with serum prostate specific antigen (PSA). Limitations in CaP diagnosis and characterisation may be overcome by use of naturally-produced, prostate-specific biofluids, such as ejaculate and post-ejaculate urethral washings (PEUW). Ejaculate is known to contain prostatic cells and relevant molecules in the non-cellular component, while PEUW may contain these as well as biomarkers reflective of systemic changes due to or causing CaP.The overall aim of this thesis was to use molecular and metabolomic nuclear magnetic resonance (NMR)-based detection methods to study prostatic fluid derived from ejaculate and PEUW to improve CaP diagnosis. Specifically, this thesis sought to optimise ejaculate sample processing for metabolomics studies and evaluate the diagnostic performance of mRNA, microRNA and metabolomic markers in ejaculate to complement serum PSA in detecting clinically significant CaP. Furthermore, this thesis investigated the feasibility and performance of PEUW-based mRNA and metabolomic biomarker performance attributable to the presence of ejaculate in urine to reflect local prostatic and systemic alterations in order to more accurately detect clinically significant CaP. Dependent clinical variables considered were absolute (positive/negative) and clinically significant (present/absent) CaP as well as risk groups (low, intermediate, high) according to the D'Amico criteria.Using a NMR-based metabolomics enzyme kinetics study design, the addition of tartrate and cooling of ejaculate samples improved the stability of choline and phosphorylcholine concentrations. Sample collection into a sterile urine jar containing 5 mM (on-site) or 10 mM (off-site) tartrate in 20 ml PBS solution cooled to 277 K and cooled during transport until processing would result in at most a 2-3% change in choline and phosphorylcholine to facilitate sample collection off-site without significant effect on choline-based metabolites.Following prostatic cell RNA isolation, amplification and qPCR for β2-microglobulin (β2M), PSA, PCA3 and Hepsin in ejaculate, adequate RNA for all assays was obtained for 66 patients and determined that a Hepsin:PCA3 ratio in ejaculate together with serum PSA best predicted absolute CaP (AUC= 0.724 vs 0.676) and csCaP (AUC= 0.701 vs 0.680).iii Matched mRNA and microRNA expression was possible for a subgroup of patients (n=20) Lipids/lipoproteins dominated spectra of high grade samples. Overall CaP prediction using metabolites described in previous studies was not validated. However, findings suggest that incorporation of in vitro NMR-based metabolomics may translate to in v...

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“…Although genomics and proteomics can provide important informa tion on the expected function, metabolomics provides an immediate snapshot of all biological functions that reflect current events at an exact moment [81,82] . By measuring the population of biomarkers (metabolites), it may be possible to distinguish the profiles or signatures of healthy individuals from those of persons with specific diseases [83] . Moreover, metabolomics may provide evidence of a metabolic disorder or injury with a high degree of precision and at less cost than by means of genomics, transcriptomics or proteomics.…”

Section: Metabolomicsmentioning

confidence: 99%

Understanding the pathogenesis of hip fracture in the elderly, osteoporotic theory is not reflected in the outcome of prevention programmes

Guerado

Sandalio

Caracuel

et al. 2016

WJO

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High resolution 1H NMR spectroscopic studies on dynamic biochemical processes in incubated human seminal fluid samples

Cited by 59 publications

References 22 publications

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Multiple markers for the non-invasive diagnosis and characterisation of prostate cancer

Understanding the pathogenesis of hip fracture in the elderly, osteoporotic theory is not reflected in the outcome of prevention programmes

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