High prevalence of viruses in table grape from Spain detected by real-time RT-PCR

Bertolini, Edson; García, Julio; Yuste, Alberto; Olmos, Antonio

doi:10.1007/s10658-010-9663-4

Cited by 33 publications

(25 citation statements)

References 10 publications

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“…The infection of this old cultivar with GLRaV-1 and GLRaV-3 is in accordance with previous results, since a high level of incidence of these viruses was detected in Alicante province in other table grapes cultivars (Bertolini et al, 2010).…”

Section: Discussionsupporting

confidence: 92%

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

Peiró

Gammoudi

Yuste

et al. 2015

Span. j. agric. res.

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<p>The conservation of old grapevine varieties is important since they are adapted to specific climate conditions and may carry genes interesting to breeders. As virus infection is common in grapevine varieties, the use of virus free materials is of great importance. In this work, we used somatic embryogenesis for the sanitation of GLRaV-1 and GLRaV-3 viruses that were found after analyzing the putative presence of the five most common, economically important grape viruses by real-time multiplex RT-PCR in the old cultivar “Grumet Negre”. Unopened and opened inflorescences, fecundated ovaries, and, also, mature seeds were used as starting explants. Explants were<em> </em>cultured on plates with two embryogenesis induction media (Nitsch & McCown Woody plant medium) that contained the growth regulator thidiazuron and differed in their salt and vitamin compositions. One half of each kind of explant was cut prior to being cultured. After five months of culture, embryos had only developed from seeds that were cut previous to sowing. To the best of our knowledge, this is the first time that mature seeds have been used for inducing embryogenesis in grape. A total of 42% of the embryos transferred to tubes for germination regenerated into normal plantlets. The absence of both the GLRaV-1 and GLRaV-3 viruses in all regenerated plants was confirmed by real-time uniplex RT-PCR. So, this protocol can be used for sanitation and also for micropropagation.</p>

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Section: Discussionsupporting

confidence: 92%

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

Peiró

Gammoudi

Yuste

et al. 2015

Span. j. agric. res.

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“…Low presence of GLRaV‐2 and GVB agrees with their detection ratio in other regions of Italy, whereas the absence of ArMV confirms the rare occurrence of this virus (Bertolini et al. ).…”

Section: Resultssupporting

confidence: 84%

“…The high percentage of infected varieties, equal to 94.5%, shows a sanitary status of indigenous germplasm similar to other countries such as Spain (96%) (Bertolini et al. ) and other Italian regions such as Sicily (98%), Campania (87%) and Sardinia (77%) (Garau et al. ; Barba et al.…”

Section: Resultsmentioning

confidence: 56%

Grapevine Viruses' Detection and Sanitary Selection in Grapevine Germplasm of Calabria (Southern Italy)

Leo

Luison

Grande

et al. 2014

Journal of Phytopathology

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A survey of grapevine viruses present in the region of Calabria (southern Italy) was carried out, and the sanitary selection was conducted on various indigenous varieties. Serological (ELISA) and molecular (multiplex RT‐PCR) tests were used to detect the viruses included in the Italian certification programme: Arabis mosaic virus (ArMV), Grapevine fanleaf virus (GFLV), Grapevine leafroll associated virus 1 (GLRaV‐1), Grapevine leafroll associated virus 2 (GLRaV‐2), Grapevine leafroll associated virus 3 (GLRaV‐3), Grapevine virus A (GVA), Grapevine virus B (GVB) and Grapevine fleck virus (GFkV). The frequency with which the above viruses have been detected was 37.4, 32.6, 12.8, 7.7, 7.3, 1.9 and 0.3%, respectively, for GVA, GLRaV‐3, GFLV, GFKV, GLRaV‐1, GLRaV‐2 and GVB. ArMV was never found. The sanitary selection allowed for the detection of 6 putative clones of ‘Arvino’, 2 of ‘Magliocco dolce’ and 2 of the rootstock ‘17–37’ free of the above‐mentioned viruses. The necessary process for the commercialization of these clones as ‘certified’ propagation material was accomplished, and their official approval by the Italian Ministry of Agriculture is currently in progress.

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“…In contrast, the rate of positive samples for GLRaV‐1 and GLRaV‐3 in Spain was 66.3% and 94.7%, respectively (Bertolini et al. ). Survey of grapevine viruses in Chile also revealed high prevalence of GLRaV‐1 (4.67%), GLRaV‐2 (16.05%), GLRaV‐3 (6.41%) and GVA (5.57%) (Fiore et al.…”

Section: Resultsmentioning

confidence: 98%

“…It is difficult to estimate the level of damage produced by this virus because of the latency of the infection, even though it is quite frequent in all the areas where grapes are cultivated (Bertolini et al. ; Fiore et al. ).…”

Section: Resultsmentioning

confidence: 99%

Detection of Grapevine Viruses in Poland

Komorowska

Berniak

Golis

2013

Journal of Phytopathology

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During a 3-year study, grapevines from 23 vineyards in Poland were surveyed for virus diseases and tested to determine the prevalence of the most economically important viruses by RT-PCR. The rate of positive samples was 2.2% for grapevine leafroll-associated virus 1 (GLRaV-1), 1.9% for grapevine leafroll-associated virus 2 (GLRaV-2), 1.5% grapevine leafrollassociated virus 3 (GLRaV-3), 1.9% for grapevine virus A (GVA), 0.2% for grapevine virus B (GVB), 0.2% for grapevine virus E (GVE), 0.65% for grapevine fanleaf virus (GFLV), 20.4% for grapevine fleck virus (GFkV) and 71.9% for grapevine rupestris stem pitting-associated virus (GRSPaV). These viruses were found to occur as single or mixed infections of different combinations in individual grapevines. The overall viral infection rate in the surveyed grapevines was 82.6%. GRSPaV is the most widely distributed virus of all the viruses currently detected in the region. DNA sequencing confirmed the identification of the viruses in selected samples, and analysis indicated that the Polish isolates shared a close molecular identity with the corresponding isolates in GenBank. To our knowledge, this is the first detection of GLRaV

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High prevalence of viruses in table grape from Spain detected by real-time RT-PCR

Cited by 33 publications

References 10 publications

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

Grapevine Viruses' Detection and Sanitary Selection in Grapevine Germplasm of Calabria (Southern Italy)

Detection of Grapevine Viruses in Poland

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