The aim of this study was to determine selected phytochemicals in berries of eight sea buckthorn (Hippophaë rhamnoides subsp. mongolica) cultivars, including lipophilic and hydrophilic compounds. In the experiment chromatographic analyses, GC (phytosterols and fatty acids), UPLC-PDA-FL, LC-MS (polyphenols), and HPLC (L-ascorbic acid), as well spectrophotometric method (total carotenoids) were used. The lipid fraction isolated from whole fruit contained 14 phytosterols (major compounds β-sitosterol > 24-methylenecykloartanol > squalene) and 11 fatty acids in the order MUFAs > SFAs > PUFAs. Carotenoids occurred in concentrations between 6.19 and 23.91 mg/100 g fresh weight (fw) (p < 0.05). The major polyphenol group identified in berries was flavonols (mean content of 311.55 mg/100 g fw), with the structures of isorhamnetin (six compounds), quercetin (four compounds), and kaempferol (one compound) glycosides. Examined sea buckthorn cultivars were characterized also by a high content of L-ascorbic acid in a range from 52.86 to 130.97 mg/100 g fw (p < 0.05).

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Phytochemical profiles of four different honeysuckle varieties and four genotypes were studied. Polyphenols were identified by LC-PDA-QTOF/MS and quantified by UPLC-PDA and UPLC-FL. A total of 21 polyphenolic compounds found in the investigated fruit tissues were identified and presented as 6 anthocyanins, 6 flavan-3-ols, 4 phenolic acids, 3 flavanols, and 2 flavones. Among the identified compounds polymeric procyanidins and one luteolin derivative were quantified for the first time. Anthocyanins and flavan-3-ols were the major classes of honeysuckle polyphenols. The content of total polyphenols was between 775 mg (genotype 'Klon 38') and 2005 mg/100 g dry matter (cultivar 'Duet'). The content of ascorbic acid ranged from 3.19 to 32.12 mg/100 g fresh matter for genotypes 'Klon C' and 'Klon 44', respectively. The content of polyphenolic compounds was highly correlated with the antioxidant activity. Some honeysuckle genotypes may be deemed interesting as applicable in human nutrition.

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