Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

Peiró, Rosa; Gammoudi, Najet; Yuste, Alberto; Olmos, Antonio; Gisbert, Carmina

doi:10.5424/sjar/2015132-7094

Cited by 14 publications

(9 citation statements)

References 35 publications

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“…The presence of the viruses Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV), Grapevine fleck virus (GFkV), Grapevine leafroll associated virus-1 (GLRaV-1), and Grapevine leafroll associated virus-3 (GLRaV-3) was evaluated. Leaf extractions were performed like in Peiró et al (2015). The real-time multiplex RT-PCR was performed according to López-Fabuel et al (2013) for the simultaneous detection of ArMV, GFLV, GFkV, GLRaV-1, and GLRaV-3 viruses.…”

Section: Virus Analysismentioning

confidence: 99%

Recovering ancient grapevine cultivars in the Spanish provinces of Alicante and Valencia

Yuste¹,

Martínez‐Gil²,

García‐Martínez³

et al. 2019

Acta Hortic.

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The provinces of Alicante and Valencia, located in the Mediterranean coast of Spain, are important viticulture areas since historic times. In the context of the research project CGL2015-70843-R, we initiated different approaches in order to recover ancient varieties threatened of disappearance and we developed in vitro protocols for virus sanitation and in vitro conservation of the recovered germplasm. Among the historic varieties, we localized several accessions of varieties that were commonly grown in the pre-phylloxera era in the provinces of Alicante and Valencia (i.e. 'Valencí blanc', 'Valencí negre', 'Planta Mula', 'Botó de Gall' or 'Raïm del Clotet'). Microsatellite profiles were obtained to confirm or identify the surveyed germplasms and genetic variability was observed. In addition, a survey was carried out covering the main area of 'Monastrell' cultivation in the Vinos de Alicante Protected Designation of Origin. This ancient variety, also known as 'Mourvèdre', is cultivated mainly in the southeast of Spain and it is highly adapted to the dry and warm climate of this area. Genotyping by Sequencing (GBS) was used to estimate the genetic diversity of this variety and high variability was found. This analysis will provide a high number of high quality SNPs well distributed across the genome suitable for genotyping of clones, which will allow the design of strategies optimizing their conservation and use.

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Section: Virus Analysismentioning

confidence: 99%

Recovering ancient grapevine cultivars in the Spanish provinces of Alicante and Valencia

Yuste¹,

Martínez‐Gil²,

García‐Martínez³

et al. 2019

Acta Hortic.

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“…The first results for SE based elimination of viruses from grapevine were published by Goussard and coworkers [ 14 ], where grapevine leafroll associated viruses (GLRaV-2 and -3) were eliminated from ‘Roobernet’ cultivar. Using this method, several other viruses and even viroids were successfully eliminated from different cultivars [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ]. SE protocols vary according to the tissue explant, growth regulator type and concentration, and the direct or indirect somatic embryogenesis strategy (see Table S1 for details and references).…”

Section: Introductionmentioning

confidence: 99%

HTS-Based Monitoring of the Efficiency of Somatic Embryogenesis and Meristem Cultures Used for Virus Elimination in Grapevine

Turcsán¹,

Demián

Varga

et al. 2020

Plants

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Meristem culture and somatic embryogenesis are effective tools for virus elimination of vegetatively propagated crops including grapevine (Vitis vinifera L.). While both have been shown to be useful to eliminate the main grapevine viruses, their efficiency differs depending on the virus and grapevine variety. In our work, we investigated the efficiency of these two virus elimination methods using small RNA high-throughput sequencing (HTS) and RT-PCR as virus diagnostics. Field grown mother plants of four clones representing three cultivars, infected with different viruses and viroids, were selected for elimination via somatic embryogenesis (SE) and meristem culture (ME). Our results show for the first time that using SE, elimination in mother plants was effective for all viruses, i.e., grapevine rupestris vein feathering virus (GRVFV), grapevine Syrah virus 1 (GSyV-1), Grapevine virus T (GVT) and grapevine Pinot gris virus (GPGV). This study also confirms previous studies showing that SE is a possible strategy for the elimination of GFkV, GRSPaV, HSVd, and GYSVd-1. Our results demonstrate that the efficacy of virus elimination via SE is relatively high while the purging of viroids is lower. Our work provides evidence that the efficiency of SE is comparable to that of the technically difficult ME technique, and that SE will offer a more effective strategy for the production of virus-free grapevine in the future.

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“…Since the middle late of the twentieth century, meristem culture and thermotherapy were applied with this aim for grapevine sanitation [37,38]. Other techniques such as chemotherapy, electrotherapy, cryotherapy, and somatic embryogenesis were also reported [39][40][41].…”

Section: Grapevine Preservationmentioning

confidence: 99%

“…The other methodology used for virus sanitation was the induction of somatic embryos as reported in Peiró et al [41]. Briefly, seeds of "Valencí Blanc" were extracted from grapes and disinfected and cut previously to be cultured on the embryogenesis induction medium (EIM2) which contained TDZ (thidiazuron) at 0.9 μM.…”

Section: Virus Analysis and Sanitationmentioning

confidence: 99%

Recovering Ancient Grapevine Varieties: From Genetic Variability to In Vitro Conservation, A Case Study

Gisbert¹,

Peiró²,

Pedro³

et al. 2018

Grapes and Wines - Advances in Production, Processing, Analysis and Valorization

Self Cite

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A great number of varieties have been described in grapevine; however, few of them are currently in use. The increasing concern on varietal diversity loss has encouraged actions for recovering and preserving grapevine germplasm, which represents valuable resources for breeding as well as for diversification in grapevine-derived products. On the other hand, it is expected that this important crop, which is distributed in warm areas worldwide, will suffer the climate changes. Therefore, it is also convenient the identification of intravarietal variability and the recovery of accessions well adapted to particular environments. In this chapter, we will contribute to highlight the importance of recovering ancient materials, the usefulness of SSR markers to determine their molecular profile, the importance to analyze their virus status, and the possibilities that offer biotechnological tools for virus sanitation and in vitro storage as a complement of field preservation. In this context, we have evaluated different grapevine accessions and developed in vitro culture protocols for micropropagation, sanitation, and storage grapevine cultivars. In this work, we report the results obtained for the historic variety "Valencí Blanc" (or "Beba") and the historic and endangered variety "Esclafagerres" ("Esclafacherres" or "Esclafacherris").

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Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

Cited by 14 publications

References 35 publications

Recovering ancient grapevine cultivars in the Spanish provinces of Alicante and Valencia

Recovering ancient grapevine cultivars in the Spanish provinces of Alicante and Valencia

HTS-Based Monitoring of the Efficiency of Somatic Embryogenesis and Meristem Cultures Used for Virus Elimination in Grapevine

Recovering Ancient Grapevine Varieties: From Genetic Variability to In Vitro Conservation, A Case Study

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