High prevalence and lack of diversity of Wolbachia pipientis in Aedes albopictus populations from Northeast Brazil

Albuquerque, Alessandra Lima de; Magalhães, Tereza; Ayres, Constância Flávia Junqueira

doi:10.1590/s0074-02762011000600021

Cited by 25 publications

(20 citation statements)

References 27 publications

(41 reference statements)

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“…The other explanation could be these genes might have some role in CI, resulting in a high selective pressure that might hinder the occurrence of new strains (Albuquerque et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Distribution and dynamics of Wolbachia infection in Malaysian Aedes albopictus

et al. 2015

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“…The other explanation could be these genes might have some role in CI, resulting in a high selective pressure that might hinder the occurrence of new strains (Albuquerque et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Distribution and dynamics of Wolbachia infection in Malaysian Aedes albopictus

et al. 2015

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“…Wolbachia is commonly found in Ae. albopictus (de Albuquerque, Magalhaes, & Ayres, 2011; Joanne et al, 2015; Kitrayapong, Baimai, & O’Neill, 2002), but until recently Ae. aegypti was thought not to carry this bacterium (Gloria-Soria, Chiodo, & Powell, 2018; Kitrayapong et al, 2002; Kittayapong, Baisley, Baimai, & O’Neill, 2000).…”

Section: Introductionmentioning

confidence: 99%

Wolbachia pipientisoccurs inAedes aegyptipopulations in New Mexico and Florida, USA

Kulkarni

Jiang

et al. 2018

Preprint

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The mosquitoes Aedes aegypti (L.) and Ae. albopictus Skuse are the major vectors of dengue, Zika, yellow fever and chikungunya viruses worldwide. Wolbachia, an endosymbiotic bacterium present in many insects, is being utilized in novel vector control strategies to manipulate mosquito life history and vector competence to curb virus transmission. Earlier studies have found that Wolbachia is commonly detected in Ae. albopictus but rarely detected in Ae. aegypti. In this study, we used a two-step PCR assay to detect Wolbachia in wild-collected samples of Ae. aegypti. The PCR products were sequenced to validate amplicons and identify Wolbachia strains. A loop-mediated isothermal amplification (LAMP) assay was developed and used for detecting Wolbachia in selected mosquito specimens as well. We found Wolbachia in 85/148 (57.4%) wild Ae. aegypti specimens from various cities in New Mexico and in 2/46 (4.3%) from St. Augustine, Florida. We did not detect Wolbachia in 94 samples of Ae. aegypti from Deer Park, Harris County, Texas. Wolbachia detected in Ae. aegypti from both New Mexico and Florida was the wAlbB strain of Wolbachia pipientis. A Wolbachia positive colony of Ae. aegypti was established from pupae collected in Las Cruces, New Mexico in 2018. The infected females of this strain transmitted Wolbachia to their progeny when crossed with males of Rockefeller strain of Ae. aegypti, which does not carry Wolbachia. In contrast, none of the progeny of progeny of Las Cruces males mated to Rockefeller females were infected with Wolbachia.

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“…albopictus (de Albuquerque, Magalhaes, & Ayres, 2011;Joanne et al, 2015;Kitrayapong, Baimai, & O'Neill, 2002), but until recently Ae. (Stegomyia) albopictus Skuse are the major vectors for the transmission of several arthropod-borne viruses (arboviruses) among humans, particularly dengue, Zika, yellow fever, and chikungunya viruses.…”

mentioning

confidence: 99%

“…Wolbachia are commonly found in Ae. albopictus (de Albuquerque, Magalhaes, & Ayres, 2011;Joanne et al, 2015;Kitrayapong, Baimai, & O'Neill, 2002), but until recently Ae. aegypti was thought not to carry this bacterium (Gloria-Soria, Chiodo, & Powell, 2018;Kitrayapong et al, 2002;Kittayapong, Baisley, Baimai, & O'Neill, 2000).…”

mentioning

confidence: 99%

Wolbachia pipientis occurs in Aedes aegypti populations in New Mexico and Florida, USA

Kulkarni

Jiang

et al. 2019

Ecology and Evolution

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The mosquitoes Aedes aegypti (L.) and Ae. albopictus Skuse are the major vectors of dengue, Zika, yellow fever, and chikungunya viruses worldwide. Wolbachia, an endosymbiotic bacterium present in many insects, is being utilized in novel vector control strategies to manipulate mosquito life history and vector competence to curb virus transmission. Earlier studies have found that Wolbachia is commonly detected in Ae. albopictus but rarely detected in Ae. aegypti. In this study, we used a two‐step PCR assay to detect Wolbachia in wild‐collected samples of Ae. aegypti. The PCR products were sequenced to validate amplicons and identify Wolbachia strains. A loop‐mediated isothermal amplification (LAMP) assay was developed and used for detecting Wolbachia in selected mosquito specimens as well. We found Wolbachia in 85/148 (57.4%) wild Ae. aegypti specimens from various cities in New Mexico, and in 2/46 (4.3%) from St. Augustine, Florida. Wolbachia was not detected in 94 samples of Ae. aegypti from Deer Park, Harris County, Texas. Wolbachia detected in Ae. aegypti from both New Mexico and Florida was the wAlbB strain of Wolbachia pipientis. A Wolbachia‐positive colony of Ae. aegypti was established from pupae collected in Las Cruces, New Mexico, in 2018. The infected females of this strain transmitted Wolbachia to their progeny when crossed with males of Rockefeller strain of Ae. aegypti, which does not carry Wolbachia. In contrast, none of the progeny of Las Cruces males mated to Rockefeller females were infected with Wolbachia.

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High prevalence and lack of diversity of Wolbachia pipientis in Aedes albopictus populations from Northeast Brazil

Cited by 25 publications

References 27 publications

Distribution and dynamics of Wolbachia infection in Malaysian Aedes albopictus

Distribution and dynamics of Wolbachia infection in Malaysian Aedes albopictus

Wolbachia pipientisoccurs inAedes aegyptipopulations in New Mexico and Florida, USA

Wolbachia pipientis occurs in Aedes aegypti populations in New Mexico and Florida, USA

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