<i>Wolbachia pipientis</i>occurs in<i>Aedes aegypti</i>populations in New Mexico and Florida, USA

Kulkarni, Aditi; Yu, Wanqin; Jiang, Jinjin; Sanchez, Concepcion; Karna, Ajit K.; Martinez, Kalli J.L.; Hanley, Kathryn A.; Buenemann, Michaela; Hansen, Immo A.; Xue, Rui‐De; Ettestad, Paul; Melman, Sandra D.; Duguma, Dagne; Debboun, Mustapha; Xu, Jianchu

doi:10.1101/455014

Cited by 6 publications

(21 citation statements)

References 41 publications

(43 reference statements)

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“…Through these analyses, we demonstrate conclusively that the LC population does not harbor Wolbachia. These results conflict with those from the original study (Kulkarni et al, 2019) and more recent tests by the authors…”

Section: Te S Ting a Putatively Wolbach Ia-infec Ted L Abor Atory Pcontrasting

confidence: 98%

“…These studies report variable infection frequencies in populations and identify infections from several Wolbachia supergroups. Most studies found that the infections detected were closely related to or identical to the wAlbB infection that occurs natively in Aedes albopictus (Balaji et al, 2019;Carvajal et al, 2019;Coon et al, 2016;Kulkarni et al, 2019), while other studies also detected Wolbachia from supergroups that do not normally occur within Diptera (Carvajal et al, 2019;Thongsripong et al, 2018). Most evidence is limited to molecular detection, and not all studies claim to have discovered an active infection.…”

Section: De Tec Ti On S Of Wolbach Ia In a Ed E S Aegyptimentioning

confidence: 99%

“…We obtained one of these populations with the intention of examining crossing patterns between natural infections and novel infections that are being deployed into the field (Ant et al, 2018;Walker et al, 2011). An A. aegypti population from Las Cruces, New Mexico, USA, was established in the laboratory in September 2018 (Kulkarni et al, 2019) and kindly provided to us by the authors. We received eggs from the third and fourth generations of this population (denoted LC) which were hatched and maintained in our laboratory according to methods described previously (Ross, Axford, Richardson, Endersby-Harshman, & Hoffmann, 2017 aegypti (Axford et al, 2016) were included as negative and positive controls, respectively, in each assay.…”

Section: Te S Ting a Putatively Wolbach Ia-infec Ted L Abor Atory Pmentioning

confidence: 99%

“…TaqMan probe assays were performed as described previously Mee et al, 2015), targeting the 16S rDNA (Supporting Information Appendix S1). We also performed conventional PCR with 16S rDNA and gatB primers following methods described by the authors of the original study (Kulkarni et al, 2019). Finally, LAMP assays were performed using our protocol (Jasper et al, 2019) but with primers used to diagnose Wolbachia infections by the original study (Kulkarni et al, 2019).…”

Section: Te S Ting a Putatively Wolbach Ia-infec Ted L Abor Atory Pmentioning

confidence: 99%

“…We also performed conventional PCR with 16S rDNA and gatB primers following methods described by the authors of the original study (Kulkarni et al, 2019). Finally, LAMP assays were performed using our protocol (Jasper et al, 2019) but with primers used to diagnose Wolbachia infections by the original study (Kulkarni et al, 2019). From analyses of 72 individuals from both generations with the three molecular assays, zero were infected (Supporting Information Appendix S2).…”

Section: Te S Ting a Putatively Wolbach Ia-infec Ted L Abor Atory Pmentioning

confidence: 99%

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An elusive endosymbiont: Does Wolbachia occur naturally in Aedes aegypti?

Ross

Callahan

Yang

et al. 2020

Ecology and Evolution

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Wolbachia are maternally inherited endosymbiotic bacteria found within many insect species. Aedes mosquitoes experimentally infected with Wolbachia are being released into the field for Aedes‐borne disease control. These Wolbachia infections induce cytoplasmic incompatibility which is used to suppress populations through incompatible matings or replace populations through the reproductive advantage provided by this mechanism. However, the presence of naturally occurring Wolbachia in target populations could interfere with both population replacement and suppression programs depending on the compatibility patterns between strains. Aedes aegypti were thought to not harbor Wolbachia naturally but several recent studies have detected Wolbachia in natural populations of this mosquito. We therefore review the evidence for natural Wolbachia infections in A. aegypti to date and discuss limitations of these studies. We draw on research from other mosquito species to outline the potential implications of natural Wolbachia infections in A. aegypti for disease control. To validate previous reports, we obtained a laboratory population of A. aegypti from New Mexico, USA, that harbors a natural Wolbachia infection, and we conducted field surveys in Kuala Lumpur, Malaysia, where a natural Wolbachia infection has also been reported. However, we were unable to detect Wolbachia in both the laboratory and field populations. Because the presence of naturally occurring Wolbachia in A. aegypti could have profound implications for Wolbachia‐based disease control programs, it is important to continue to accurately assess the Wolbachia status of target Aedes populations.

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Section: Te S Ting a Putatively Wolbach Ia-infec Ted L Abor Atory Pcontrasting

confidence: 98%

Section: De Tec Ti On S Of Wolbach Ia In a Ed E S Aegyptimentioning

confidence: 99%

Section: Te S Ting a Putatively Wolbach Ia-infec Ted L Abor Atory Pmentioning

confidence: 99%

Section: Te S Ting a Putatively Wolbach Ia-infec Ted L Abor Atory Pmentioning

confidence: 99%

Section: Te S Ting a Putatively Wolbach Ia-infec Ted L Abor Atory Pmentioning

confidence: 99%

See 3 more Smart Citations

An elusive endosymbiont: Does Wolbachia occur naturally in Aedes aegypti?

Ross

Callahan

Yang

et al. 2020

Ecology and Evolution

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Larval habitat determines the bacterial and fungal microbiota of the mosquito vector Aedes aegypti

Zouache

Martin

Rahola

et al. 2022

FEMS Microbiology Ecology

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Mosquito larvae are naturally exposed to microbial communities present in a variety of larval development sites. Several earlier studies have highlighted that the larval habitat influences the composition of the larval bacterial microbiota. However, little information is available on their fungal microbiota, i.e. the mycobiota. In this study, we provide the first simultaneous characterization of the bacterial and fungal microbiota in field-collected Aedes aegypti larvae and their respective aquatic habitats. We evaluated whether the microbial communities associated with the breeding site may affect the composition of both the bacterial and fungal communities in Ae. aegypti larvae. Our results show a higher similarity in microbial community structure for both bacteria and fungi between larvae and the water in which larvae develop than between larvae from different breeding sites. This supports the hypothesis that larval habitat is a major factor driving microbial composition in mosquito larvae. Since the microbiota plays an important role in mosquito biology, unravelling the network of interactions that operate between bacteria and fungi is essential to better understand the functioning of the mosquito holobiont.

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Rapid tissue-specific screening ofWolbachia,CardiniumandRickettsiain Flies (Diptera: Sepsidae; Drosophilidae)

Huicong

Puniamoorthy

2021

Preprint

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Maternally transmitted endosymbionts can negatively influence the reproduction of their arthropod hosts (e.g. male-killing, cytoplasmic incompatibility). However, such infections are rarely assessed in insect models such as Sepsidae or Drosophilidae that are routinely used in sexual selection studies. To detect infection and bacterial localisation in the host, we developed and optimised a tissue-specific multiplex screening protocol for Wolbachia, Cardinium and Rickettsia that can be completed in a day. The robustness of the protocol was tested with the screening of multiple species and populations of flies commonly used in reproductive studies (N=147 flies; n=426 tissues). With triplex PCRs and more effective duplex PCRs, we detected both single and co-infections in most individuals from both families (Drosophilidae | Sepsidae; Single infection: 51.4% | 62.7%; Dual infection: 29.2% | 9.3%; Triple infection: 4.2% | 0%). Surprisingly, we documented the presence of all three reproductive bacteria in 32 wild-caught drosophilids from Singapore. Also, we note that most sepsid populations (19 out of 22) tested positive for Cardinium. We found that the Rickettsia infection was overall low, but it was predominantly detected in the gastrointestinal tract instead of the reproductive tract, suggesting a potential horizontal transmission. Finally, we found that amplicon sequences of equivalent sizes between the three tissues from the same individuals share at least 98.8% identity, which suggests that the same endosymbiont strain inhabits within the whole arthropod. Overall, we believe this protocol is effective in detecting co-infections and understanding the transmission of various reproductive endosymbionts. It can also be used to assess endosymbiont infections in other insects.

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Wolbachia pipientisoccurs inAedes aegyptipopulations in New Mexico and Florida, USA

Cited by 6 publications

References 41 publications

An elusive endosymbiont: Does Wolbachia occur naturally in Aedes aegypti?

An elusive endosymbiont: Does Wolbachia occur naturally in Aedes aegypti?

Larval habitat determines the bacterial and fungal microbiota of the mosquito vector Aedes aegypti

Rapid tissue-specific screening ofWolbachia,CardiniumandRickettsiain Flies (Diptera: Sepsidae; Drosophilidae)

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