High Pressure Response of Lactic Acid Bacteria

Vogel, Rudi F.; Ehrmann, M. A.; Gänzle, Michael G.; Kato, C.; Korakli, Maher; Scheyhing, Carsten H.; Molina-Gutierrez, Adriana; Ulmer, Helge M.; Winter, Roland

doi:10.1007/978-3-662-05613-4_44

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…The denaturation of proteins and impairment of membrane functionality have been discussed as reasons for the inactivation of microorganisms (32). For many proteins, it has been described that pressure induces structural changes and denaturation, which follow elliptic pressure/temperature phase diagrams with areas of pressure stabilization of a protein at a denaturing temperature.…”

Section: Vol 72 2006 Pressure Stabilization Of C Botulinum Endospomentioning

confidence: 99%

High-Pressure-Mediated Survival of Clostridium botulinum and Bacillus amyloliquefaciens Endospores at High Temperature

Margosch

Ehrmann

Buckow

et al. 2006

Appl Environ Microbiol

202

142

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Endospores of proteolytic type B Clostridium botulinum TMW 2.357 and Bacillus amyloliquefaciens TMW 2.479 are currently described as the most high-pressure-resistant bacterial spores relevant to food intoxication and spoilage in combined pressure-temperature applications. The effects of combined pressure (0.1 to 1,400 MPa) and temperature (70 to 120°C) treatments were determined for these spores. A process employing isothermal holding times was established to distinguish pressure from temperature effects. An increase in pressure (600 to 1,400 MPa) and an increase in temperature (90 to 110°C) accelerated the inactivation of C. botulinum spores. However, incubation at 100°C, 110°C, or 120°C with ambient pressure resulted in faster spore reduction than treatment with 600 or 800 MPa at the same temperature. This pressure-mediated spore protection was also observed at 120°C and 800, 1,000, or 1,200 MPa with the more heat-tolerant B. amyloliquefaciens TMW 2.479 spores. Inactivation curves for both strains showed a pronounced pressure-dependent tailing, which indicates that a small fraction of the spore populations survives conditions of up to 120°C and 1.4 GPa in isothermal treatments. Because of this tailing and the fact that pressure-temperature combinations stabilizing bacterial endospores vary from strain to strain, food safety must be ensured in case-by-case studies demonstrating inactivation or nongrowth of C. botulinum with realistic contamination rates in the respective pressurized food and equipment.High hydrostatic pressure (HHP) is a powerful tool for inactivating food pathogens and spoilage organisms and thus achieving food safety, with flavors, colors, and nutrients being affected to a lesser extent than in conventional heat treatments (6, 28). It is generally assumed that the introduction of HHP to a heat-mediated inactivation process enhances the inactivation of bacterial endospores compared to heat treatment alone and that the rise of pressure or temperature will result in accelerated and steady inactivation. This is of particular interest because bacterial endospores are not inactivated by pressure treatment at ambient temperature, and endospores of the genera Bacillus and Clostridium tolerate pressures over 1,000 MPa at 25°C (28). For inactivation of these organisms, combinations of high pressure and heat are required (27).Changes in pressure are thermodynamically coupled to changes in temperature, and the adiabatic heating of samples during compression makes it difficult to distinguish pressure effects on bacterial spores from temperature effects. Spores in a pressure-treated sample inevitably experience a dynamic temperature curve, which depends on the pressure difference, the compression rate, and the heat transfer rate from the sample to the pressure vessel. A nearly constant temperature during pressure holding times can be achieved by minimizing or eliminating heat transfer throughout the process (adiabatic treatments). With the aim of a fast, energy-saving process for the inactivation of bacter...

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Section: Vol 72 2006 Pressure Stabilization Of C Botulinum Endospomentioning

confidence: 99%

High-Pressure-Mediated Survival of Clostridium botulinum and Bacillus amyloliquefaciens Endospores at High Temperature

Margosch

Ehrmann

Buckow

et al. 2006

Appl Environ Microbiol

202

142

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“…Pressure was shown to induce multiple changes in physiology (Wouters et al 1998;Korakli et al 2002), protein expression (Welch et al 1993;Drews et al 2002;Wemekamp-Kamphuis et al 2002) and also transcription (Sato et al 1995;Ehrmann et al 2001). It causes cell injury (Ulmer et al 2002) and finally cell death (Vogel et al 2002a).…”

Section: Introductionmentioning

confidence: 99%

Barotolerance is inducible by preincubation under hydrostatic pressure, cold-, osmotic- and acid-stress conditions in Lactobacillus sanfranciscensis DSM 20451T

Scheyhing¹,

Hörmann²,

Ehrmann³

et al. 2004

Lett Appl Microbiol

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Aims: This study addresses the inducibility of barotolerance by preincubation of Lactobacillus sanfranciscensis DSM 20451 T under various sublethal stress conditions. Methods and Results: Stress conditions which reduce the growth rate of L. sanfranciscensis DSM 20451T to 10% of its maximum were determined. These conditions were met at 43, 12AE5°C, a pH value of 3AE7, 1AE9% NaCl, or 80 MPa respectively. In contrast to heat preincubation, other prestresses, including salt, cold and pressure led to an increase of barotolerance by hydrostatic pressure of 300 MPa for 30 min. Stationary-phase cells also showed an increased barotolerance. Sublethal pressure leads to enhanced heat tolerance. Conclusions: Stress response to salt, low temperature and acidic pH as well as starvation overlap with that one to high pressure by inducing barotolerance. Significance and Impact of the Study: Inactivation of bacteria by high pressure treatment is influenced by their history which modulates barotolerance. Mechanisms of barotolerance appear different from heat shock defence.

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“…Besides the natural occurrence, HHP is also applied technically to inactivate microorganisms in food, while retaining favorable product properties like texture or flavour (Matser et al, 2004), or for the sterilization of heat-sensitive pharmaceuticals (Rigaldie et al, 2002(Rigaldie et al, , 2003. The lethal effects of HHP are caused by the dissociation of macromolecular assemblies like ribosomes or the cytoskeleton and the pressure-induced denaturation of proteins Winter et al, 2007) or by alterations in membrane structure, dynamics and permeability, which lead to the failure of cell homeostasis Vogel et al, 2002). It is generally thought that the latter effects are caused by the transition of the plasma membrane to a solid-like gel phase, accompanied with the ceasing of membrane protein function (Beney and Gervais, 2001;Ulmer et al, 2002).…”

Section: Introductionmentioning

confidence: 98%

The temperature–pressure phase diagram of a DPPC–ergosterol fungal model membrane—a SAXS and FT-IR spectroscopy study

Pühse

Jeworrek

Winter

2008

Chemistry and Physics of Lipids

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High Pressure Response of Lactic Acid Bacteria

Cited by 4 publications

References 7 publications

High-Pressure-Mediated Survival of Clostridium botulinum and Bacillus amyloliquefaciens Endospores at High Temperature

High-Pressure-Mediated Survival of Clostridium botulinum and Bacillus amyloliquefaciens Endospores at High Temperature

Barotolerance is inducible by preincubation under hydrostatic pressure, cold-, osmotic- and acid-stress conditions in Lactobacillus sanfranciscensis DSM 20451T

The temperature–pressure phase diagram of a DPPC–ergosterol fungal model membrane—a SAXS and FT-IR spectroscopy study

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