Barotolerance is inducible by preincubation under hydrostatic pressure, cold-, osmotic- and acid-stress conditions in Lactobacillus sanfranciscensis DSM 20451T

Abstract

Aims: This study addresses the inducibility of barotolerance by preincubation of Lactobacillus sanfranciscensis DSM 20451 T under various sublethal stress conditions. Methods and Results: Stress conditions which reduce the growth rate of L. sanfranciscensis DSM 20451T to 10% of its maximum were determined. These conditions were met at 43, 12AE5°C, a pH value of 3AE7, 1AE9% NaCl, or 80 MPa respectively. In contrast to heat preincubation, other prestresses, including salt, cold and pressure led to an increase of barotolerance by hydrostatic pressure of 300 MPa for 30 min. Stationary-phase cells also showed an increased barotolerance. Sublethal pressure leads to enhanced heat tolerance. Conclusions: Stress response to salt, low temperature and acidic pH as well as starvation overlap with that one to high pressure by inducing barotolerance. Significance and Impact of the Study: Inactivation of bacteria by high pressure treatment is influenced by their history which modulates barotolerance. Mechanisms of barotolerance appear different from heat shock defence.

Improvement of the food safety of low acid fermented sausages by enterocins A and B and high pressure

“…It has been demonstrated that bacterial survival is greatly increased by the preceding induction of a stress response (Yousef & Courtney, 2003) and cross-protection among different stresses has been reported, e.g. acquisition of barotolerance by application of NaCl, low pH and low temperature (Scheyhing, Hormann, Ehrmann, & Vogel, 2004).…”

High pressure-sensitive gene expression in Lactobacillus sanfranciscensis

“…In recent investigations, we have used lactic acid bacteria as Gram-positive model organisms applied to food biotechnology to study HHP-induced inactivation kinetics, mechanisms of stress response, and sublethal injury followed by cell death (16).…”

Exploration of the Effects of High Hydrostatic Pressure on Microbial Growth, Physiology and Survival: Perspectives from Piezophysiology

“…43,44) Piezotolerance is inducible in Lactococcus sanfranciscensis by preincubation under hydrostatic pressure, cold or osmotic shock, and acidity. 45) Spores of Bacillus species are germinated by a variety of stimuli including nutrients, some chemicals and mechanical treatments such as abrasion and high pressure. [46][47][48] Nutrients trigger the receptors GerA, GerB and GerK in the inner membrane of the spore, followed by release of the Ca 2þ -dipicolinic acid (DPA) depot and activation of enzymes CwlJ and SleB.…”