The temperature–pressure phase diagram of a DPPC–ergosterol fungal model membrane—a SAXS and FT-IR spectroscopy study

Pressure effects on lipid membrane structure and dynamics

“…This ordering is accompanied by a lengthening of the chains and a reduction in the area occupied relative to the headgroup, hence a variety of lipid phase transitions can be induced by changing pressure. By scanning hydrostatic pressure and temperature, full pressure-temperature phase diagrams can be built up and these have been produced for a variety of lipid systems (for example, see Duesing et al, 1997;Puehse et al, 2008;Winter, 2001;Yaghmur et al, 2010).…”

Interaction of imidazolium-based lipids with phospholipid bilayer membranes of different complexity

“…Due to temperature-induced changes in the transgauche ratio of the acyl chains, a distinct change in the maximum position of this specific band was observed. 37,45 Hence, the shift in the maximum wavenumber, Dṽ, of this band can be used as a marker for the fluidity and change in phase behavior of the membrane.…”

Separation of liquid domains in model membranes induced with high hydrostatic pressure

“…18 Importantly, lipid bilayers have been shown to be significantly more responsive to hydrostatic pressure than other biomolecules such as proteins or DNA. 8,19 L d -L o phase separation has been studied as a function of pressure in multilamellar systems formed from binary 20 and ternary lipid mixtures, using FT-IR, small angle X-ray diffraction (SAXS) 19,21 and NMR. 22 However, all these methods probe the ensemble average structure.…”