Pressure effects on lipid membrane structure and dynamics

Brooks, Nicholas J.; Ces, Oscar; Templer, Richard H.; Seddon, John M.

doi:10.1016/j.chemphyslip.2010.12.002

Cited by 64 publications

(83 citation statements)

References 93 publications

(152 reference statements)

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“…However, when pressure is applied to the sample containing 30 mol% EC, the ripple phase forms at 80 MPa. Pressure will always drive formation of lower volume structures 25 and so this suggests that the ripple phase in these mixtures is a more compact structure than the flat gel phase. In the case of bovine brain sphingomyelin, hydrostatic pressure has been shown to significantly sharpen the SAXS pattern observed from the ripple phase.…”

Section: Ripple Phase (P β ')mentioning

confidence: 99%

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Pressure–Temperature Phase Behavior of Mixtures of Natural Sphingomyelin and Ceramide Extracts

et al. 2015

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Ceramides are a group of sphingolipids that act as highly important signaling molecules in a variety of cellular processes including differentiation and apoptosis. The predominant in-vivo synthetic pathway for ceramide formation is via sphingomyelinase catalyzed hydrolysis of sphingomyelin. The biochemistry of this essential pathway has been studied in detail, however there is currently a lack of information on the structural behavior of sphingomyelin and ceramide rich model membrane systems, which is essential for developing a bottom up understanding of ceramide signaling and platform formation. We have studied the lyotropic phase behavior of sphingomyelin -ceramide mixtures in excess water as a function of temperature (30 -70 °C) and pressure (1 -200 MPa) by small-and wide-angle X-ray scattering. At low ceramide concentrations the mixtures form the ripple gel phase (P β ') below the gel transition temperature for sphingomyelin and this observation has been confirmed by atomic force microscopy.Formation of the ripple gel phase can also be induced at higher temperatures via the application 2 of hydrostatic pressure. At high ceramide concentration an inverse hexagonal phase (H II ) is formed coexisting with a cubic phase.

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Section: Ripple Phase (P β ')mentioning

confidence: 99%

“…Increasing pressure generally increases ordering in lipid hydrocarbon chains 25 and tends to lead to chain extension an so an increase in lattice parameter. However in a gel phase, the chains have a high degree of conformational order and hence pressure would be expected to have a smaller effect as seen here.…”

Section: Ripple Phase (P β ')mentioning

confidence: 99%

Pressure–Temperature Phase Behavior of Mixtures of Natural Sphingomyelin and Ceramide Extracts

et al. 2015

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“…This view is supported by the noticeably larger lattice parameters of self-assemblies within lipid particles. With increasing pressure, self-assemblies in bulk state swell as expected, 34,36,41 but the ones inside lipid particles swell even more (Table 1).…”

Section: Discussionmentioning

confidence: 68%

“…The lattice parameter for the monophasic cubic Ia3d phase at atmospheric pressure under limited hydration conditions was found to be 100.9 Å by Barauskas et al 52 The characteristic Bonnet ratio for the pressureinduced coexistence of cubic Ia3d and Pn3m phases matched well with the theoretical value of 1.576 60 during upward pressure scan while a little deviation was seen during the downward pressure scan (1.50-1.58). It should be noted that the coexisting cubic Ia3d phase is not commonly seen in pure lipid systems at atmospheric pressure in excess water and for hydrostatic pressure measurements performed under excess water conditions 34,43 .…”

Section: Pressure Effect On Pt-based Bulk and Dispersed Samples: Evolmentioning

confidence: 99%

“…It should be noted that most of the earlier studies in literature were focused on the effect of pressure on equilibrium non-lamellar liquid crystalline, bulk phases. These studies involved, the effects of hydrostatic [30][31][32][33] and hydrodynamic [34][35][36][37] pressure on pure lipids and their mixtures under dry (solvent-free lipid), limited hydration, or excess water conditions. Most of these reports were focused on understanding isothermal pressure-induced structural alterations and dynamic phase transitions of the lipid crystalline and liquid crystalline phases [30][31][38][39][40][41][42] .…”

Section: Introductionmentioning

confidence: 99%

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Effects of High Pressure on Internally Self-Assembled Lipid Nanoparticles: A Synchrotron Small-Angle X-ray Scattering (SAXS) Study

et al. 2016

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We present the first report on the effect of hydrostatic pressure on colloidally stabilized lipid nanoparticles enveloping inverse non-lamellar self-assemblies in their interiors. These internal self-assemblies were systematically tuned into bicontinuous cubic (Pn3m and Im3m), micellar cubic (Fd3m), hexagonal (H2), and inverse micellar (L2) phases by regulating the lipid-oil ratio while the hydrostatic pressure was varied from atmospheric pressure to 1200 bar, and back to the atmospheric pressure. The pressure effect on these lipid nanoparticles was compared with their equilibrium bulk, non-dispersed counterparts, i.e. inverse nonlamellar liquid crystalline phases and micellar solutions under excess water conditions using synchrotron small angle X-ray scattering (SAXS) technique. In the applied pressure-range, induced phase transitions were solely observed in fully hydrated bulk samples; whereas the internal self-assemblies of the corresponding lipid nanoparticles displayed only pressuremodulated single phases. Interestingly both, the lattice parameters and linear pressure expansion coefficients were larger for the self-assemblies enveloped inside the lipid nanoparticles as compared to the bulk states. This can in part be attributed to enhanced lipid layer undulations in the lipid particles in addition to induced swelling effects in presence of the tri-block copolymer F127. The bicontinuous cubic phases in both bulk state and inside lipid cubosome nanoparticles swell on compression, even so both keep swelling further on decompression at relatively high pressures before shrinking again at ambient pressures.Pressure dependence of the phases is also modulated by the concentration of the solubilized oil (tetradecane). These studies demonstrate the tolerance of lipid nanoparticles (cubosomes, hexosomes, micellar cubosomes, and emulsified microemulsions (EMEs)) for high pressures proving their robustness for various technological applications.3

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High Hydrostatic Pressure Induces a Lipid Phase Transition and Molecular Rearrangements in Low‐Density Lipoprotein Nanoparticles

Lehofer

Golub

Kornmueller

et al. 2018

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Low-density lipoproteins (LDL) are natural lipid transporter in human plasma whose chemically modified forms contribute to the progression of atherosclerosis and cardiovascular diseases accounting for a vast majority of deaths in westernized civilizations. For the development of new treatment strategies, it is important to have a detailed picture of LDL nanoparticles on a molecular basis. Through the combination of X-ray and neutron small-angle scattering (SAS) techniques with high hydrostatic pressure (HHP) this study describes structural features of normolipidemic, triglyceride-rich and oxidized forms of LDL. Due to the different scattering contrasts for X-rays and neutrons, information on the effects of HHP on the internal structure determined by lipid rearrangements and changes in particle shape becomes accessible. Independent pressure and temperature variations provoke a phase transition in the lipid core domain. With increasing pressure an inter-related anisotropic deformation and flattening of the particle are induced. All LDL nanoparticles maintain their structural integrity even at 3000 bar and show a reversible response toward pressure variations. The present work depicts the complementarity of pressure and temperature as independent thermodynamic parameters and introduces HHP as a tool to study molecular assembling and interaction processes in distinct lipoprotein particles in a nondestructive manner.

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Pressure effects on lipid membrane structure and dynamics

Cited by 64 publications

References 93 publications

Pressure–Temperature Phase Behavior of Mixtures of Natural Sphingomyelin and Ceramide Extracts

Pressure–Temperature Phase Behavior of Mixtures of Natural Sphingomyelin and Ceramide Extracts

Effects of High Pressure on Internally Self-Assembled Lipid Nanoparticles: A Synchrotron Small-Angle X-ray Scattering (SAXS) Study

High Hydrostatic Pressure Induces a Lipid Phase Transition and Molecular Rearrangements in Low‐Density Lipoprotein Nanoparticles

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