High frequency plant regeneration of garlic (Allium sativum L.) calli immobilized in calcium alginate gel

Kim, Min A; Park, Joong Kon

doi:10.1007/bf02932971

Cited by 13 publications

(3 citation statements)

References 11 publications

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“…Calli's undifferentiated character and minimal differentiation capability restrict their adoption for explant propagules for the manufacture of synseed (Gantait et al, 2015c). For the first time, the use of calli for the formation of synseeds was successfully observed in Allium sativum (Kim & Park, 2002), exhibiting a high rate of conversion and regeneration of synseeds to plants.…”

Section: Calli and Embryogenic Masses-mentioning

confidence: 99%

Synthetic Seed Technology, Application and Future Trends

Garg¹,

Maheshwari²

2023

EIJAER

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In nature, seeds are typically the primary method of plant propagation. Some plants can be vegetatively propagated but conventional methods are time consuming, expensive and cannot produce plants at larger scale. Synthetic seed technology could play a significant role in the production of artificial seeds, or Syn-seeds. Somatic embryos lacked crucial auxiliary tissues like endosperm and protective coverings, which made them difficult to handle and store. Two kinds of somatic embryos are known as desiccated and hydrated seeds based on the techniques developed thus far. The goal of this study is to emphasize the historical and current status of the creation of synthetic seeds. The micropropagation method is still one of the main barriers to the advancement of artificial seed technology. Despite the use of somatic embryos for artificial seed generation in a variety of plant species, there are still some significant problems that must be resolved before progress can be made. The synthetic seed process is a godsend for the vegetative multiplication, conservation, & long-term conservation of rare, endangered, and vulnerable species' superior germplasm. The current scenario of artificial seed to advance agriculture innovation still requires more practical directed appliances.

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Section: Calli and Embryogenic Masses-mentioning

confidence: 99%

Synthetic Seed Technology, Application and Future Trends

Garg¹,

Maheshwari²

2023

EIJAER

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“…The capsule matrix stimulated the development of shoots in the synseeds of garlic. The encapsulated garlic calli had a significantly greater shoot length than the naked garlic calli [62]. It was noted that among four sodium alginate gel matrix concentrations studied, 3% offered the best percentages of encapsulated bulblet survival and conversion to garlic plantlets [23].…”

Section: Garlic (Allium Sativum L)mentioning

confidence: 99%

Production of Synthetic Seeds in Vegetable Crops: A Review

Abbas

Mahood

Alhasan

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Over the last few decades, biotechnology research has advanced to the point where it is now possible to improve essential agricultural crops. Synthetic seed manufacturing has opened up new possibilities of in vitro plant propagation technique since it has various practical benefits on a commercial scale for the cultivation of a wide range of agricultural crops. It is one of the most important tools for plant tissue culture breeders and scientists, since it provides significant benefits for massive production of distinct (elite) plant species. Synthetic seeds offer a promising strategy for mass plant production in a way of encapsulation of non-embryonic or embryonic (somatic embryos) tissues in a gel-like matrix, such as shoot buds, shoot tips, microshoots, protocoms and nodal segments. When the strategies minimize the cost of the production of elite plant genotypes, this strategy can be adapted for commercial production. The technology of the synthetic seed necessitates the use of in vitro growth systems in order to produce viable materials that can be converted into plants on a wide scale. The discovery of an artificial seed technology opens up new possibilities of a promising strategy for improving a wide range of commercially significant plant species, including cereals, fruit crops, medicinal plants, and vegetable crops, which we focus on in this review article.

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“…T-1 (control) 3 % Sucrose T-2 3 % Sucrose +2 mg/l JA T-3 6 % Sucrose +2 mg/l JA G= Germination, SD= Sprouting days, the singe "+" synseeds were germinate, "-" synseeds were not germinate Bekheet 2006, Mahajan et al, 2013, Paz-Robeldo and Tovar-soto 2012, Gonapathi et al, 1992and Kim and Park 2002 who reported the importance of synseeds as an alternative method of garlic micropropagation.…”

Section: Bulb Characteristicsmentioning

confidence: 99%

Evaluation the Growth Performance of Egyptian Garlic Landraces and In vitro Synseeds of Bulblets Formation

Hegazy

Gehan

elnabe

2019

Alexandria Journal of Agricultural Sciences

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Egyptian garlic cultivars suffered degenerations; lower bulb yield and smaller size of bulbs which represent a crucial problem. A survey of some Egyptian landraces to detect there genetic diversity is considered in this study. In addition, developing of simple method for bulblets regeneration and synthetic seed formation to decrease amount of clove seeds. Fourteen vegetative growth and bulb characteristics for 27 Egyptian garlic landraces were measured during two growing season to determine the phenotypic variations under condition of New Borg El-Arab, Egypt. An in vitro study was, also, carried out to determine the direct shoot induction and bulblets formation for six selected landraces and under three media protocol. The results of the field experiment revealed that, Significant differences among all studied local cultivars and ecotypes of Egyptian garlic were observed for vegetative growth, bulb and total yield characteristics. Among the studied varieties of Egyptian landraces, Borg El-Arab landrace was superior in vegetative growth as plant height (cm), leaves no./ plant, leaf length (cm), leaf width (mm), plant fresh weight (g) and foliage fresh weight (g). While, quality characters were associated with purple bulbs landraces like Egaseed and Sids-40-Ayat. Bulb dry matter percentage ranged from 36.6 to 45.68 % and from 35.07 to 44.89 % however, average cloves number per bulb was ranged from 9.33 to 40.00 and from 3.55 to 32.71 in the first and second growing seasons, respectively. The results for the invitro experiment revealed that efficient and simple method of bulblets regeneration and improving multiplication rate were developed for six selected landraces using three different media. Garlic explants (cultured on MS media) were supplemented with 6 % sucrose and 2 mg/l JA (T-3); produced significant higher mean values for numbers of bulblets /clove (12.61) and higher insignificant mean values for numbers of shoots and bulblets /explant, bulblets formation % and numbers of bulblets per clove compared with control treatment (T-1). Control treatment (T-1) produced the highest significant average bulblet weight (259.72 mg). "Salaqus-3" landrace was superior and gave the highest significant mean values for number of shoots per explant and bulblets per clove compared with other landraces. "Clone-21" produced the highest bulblet weight (232.94 mg). Synthetic seeds for six garlic landraces formed by immersing bulblets in gel matrix (3% sodium alginate solution), and exposure to calcium chloride solution (2.5 %) were germinated successfully in unsterilized soil mixture consisted of peatmoss, sand and soil (1:1:1). Conclusion: Egyptian garlic landraces showed great variability and a promising in vitro performance for plant regeneration and bulblets formation. This finding could be used as alternative propagation method, cultivars rejuvenation and germplasm conversation management along with capsulation technology.

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High frequency plant regeneration of garlic (Allium sativum L.) calli immobilized in calcium alginate gel

Cited by 13 publications

References 11 publications

Synthetic Seed Technology, Application and Future Trends

Synthetic Seed Technology, Application and Future Trends

Production of Synthetic Seeds in Vegetable Crops: A Review

Evaluation the Growth Performance of Egyptian Garlic Landraces and In vitro Synseeds of Bulblets Formation

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