Hierarchically-structured laccase@Ni3(PO4)2 hybrid nanoflowers for antibiotic degradation: Application in real wastewater effluent and toxicity evaluation

Jafari-Nodoushan, Hossein; Fazeli, Mohammad Reza; Faramarzi, Mohammad Ali; Samadi, Nasrin

doi:10.1016/j.ijbiomac.2023.123574

Cited by 9 publications

(14 citation statements)

References 99 publications

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“…For PepD-Mn-PEI@CaP, the enzyme binding to the carrier possibly limited the free conformational change of the enzyme, resulting in reduced enzyme sensitivity to temperature, thus exhibiting enhanced thermal stability. 49 In the recycled uses, PepD-Mn-PEI@CaP (Figure 6c) exhibited significantly improved recovery over PepD-PEI@ MnP (Figure S4). After 6 and 8 cycles, the remaining activities of PepD-Mn-PEI@CaP were still 96.3% and 86.8%, respectively.…”

Section: ■ Results and Discussionmentioning

confidence: 97%

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Immobilized Dipeptidase in Manganese Ion-Loaded Polyethylenimine-Induced Calcium Phosphate Nanocrystals for Carnosine Synthesis

Liu,

Yu,

Sun

2024

Langmuir

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Carnosine is a natural bioactive dipeptide with important physiological functions widely used in food and medicine. Dipeptidase (PepD) from Serratia marcescens can catalyze the reverse hydrolytic reaction of β-alanine with Lhistidine to synthesize carnosine in the presence of Mn 2+ . However, it remains challenging to practice carnosine biosynthesis due to the low activity and high cost of the enzyme. Therefore, the development of biocatalysts with high activity and stability is of significance for carnosine synthesis. Here, we proposed to chelate Mn 2+ to polyethylenimine (PEI) that induced rapid formation of calcium phosphate nanocrystals (CaP), and Mn-PEI@CaP was used for PepD immobilization via electrostatic interaction. Mn-PEI@CaP as the carrier enhanced the stability of the immobilized enzyme. Moreover, Mn 2+ loaded in the carrier acted as an in situ activator of the immobilized PepD for facilitating the biocatalytic process of carnosine synthesis. The as-prepared immobilized enzyme (PepD-Mn-PEI@CaP) kept similar activity with free PepD plus Mn 2+ (activity recovery, 102.5%), while exhibiting elevated thermal stability and pH tolerance. Moreover, it exhibited about two times faster carnosine synthesis than the free PepD system. PepD-Mn-PEI@CaP retained 86.8% of the original activity after eight cycles of batch catalysis without the addition of free Mn 2+ ions during multiple cycles. This work provides a new strategy for the coimmobilization of PepD and Mn 2+ , which greatly improves the operability of the biocatalysis and demonstrates the potential of the immobilized PepD system for efficient carnosine synthesis.

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Section: ■ Results and Discussionmentioning

confidence: 97%

“…The active site of a free enzyme is prone to deformation at high temperatures. For PepD-Mn-PEI@CaP, the enzyme binding to the carrier possibly limited the free conformational change of the enzyme, resulting in reduced enzyme sensitivity to temperature, thus exhibiting enhanced thermal stability …”

Section: Resultsmentioning

confidence: 99%

Immobilized Dipeptidase in Manganese Ion-Loaded Polyethylenimine-Induced Calcium Phosphate Nanocrystals for Carnosine Synthesis

Liu,

Yu,

Sun

2024

Langmuir

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“…This alkaline microenvironment may counteract the influence of H + in the solution [ 10 ]. As reported previously, the interaction between OH − and the copper ions of laccase could potentially impede the flow of electrons, consequently resulting in a decline in the enzyme’s activity [ 27 ]. Hence, the stability of Lac@ZIF-8-Fe was found to be inferior to that of free laccase when exposed to a weak alkaline environment (pH 7.0 to 8.0).…”

Section: Resultsmentioning

confidence: 99%

Co-Immobilization of Laccase and Mediator into Fe-Doped ZIF-8 Significantly Enhances the Degradation of Organic Pollutants

Li,

Shi,

Dong

et al. 2024

Molecules

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Co-immobilization of laccase and mediator 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) for wastewater treatment could simultaneously achieve the reusability of laccase and avoid secondary pollution caused by the toxic ABTS. Herein, Fe-induced mineralization was proposed to co-immobilize laccase and ABTS into a metal–organic framework (ZIF-8) within 30 min. Immobilized laccase (Lac@ZIF-8-Fe) prepared at a 1:1 mass ratio of Fe2+ to Zn2+ exhibited enhanced catalytic efficiency (2.6 times), thermal stability, acid tolerance, and reusability compared to free laccase. ABTS was then co-immobilized to form Lac+ABTS@ZIF-8-Fe (ABTS = 261.7 mg/g). Lac@ZIF-8-Fe exhibited significantly enhanced bisphenol A (BPA) removal performance over free laccase due to the local substrate enrichment effect and improved enzyme stability. Moreover, the Lac+ABTS@ZIF-8-Fe exhibited higher BPA removal efficiency than the free laccase+ABTS system, implying the presence of a proximity effect in Lac+ABTS@ZIF-8-Fe. In the successive malachite green (MG) removal, the MG degradation efficiency by Lac@ZIF-8-Fe was maintained at 96.6% at the fifth reuse with only an extra addition of 0.09 mM ABTS in each cycle. As for Lac+ABTS@ZIF-8-Fe, 58.5% of MG was degraded at the fifth cycle without an extra addition of ABTS. Taken together, this research has provided a novel strategy for the design of a co-immobilized laccase and ABTS system for the degradation of organic pollutants.

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“…Previous studies have shown that FQs can form multiple metabolites in organisms under the catalysis of relevant enzymes [ 17 , 18 ]. Both the parent compounds and metabolites of FQs may have toxic effects on organisms [ 19 ]. Therefore, it is necessary to further investigate the occurrence, bioaccumulation, metabolism, and toxicity of FQs in aquatic environments to determine their ecological risks.…”

Section: Introductionmentioning

confidence: 99%

Occurrence, Bioaccumulation, Metabolism and Ecotoxicity of Fluoroquinolones in the Aquatic Environment: A Review

Shen,

Hu,

Zhao

et al. 2023

Toxics

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In recent years, there has been growing concern about antibiotic contamination in water bodies, particularly the widespread presence of fluoroquinolones (FQs), which pose a serious threat to ecosystems due to their extensive use and the phenomenon of “pseudo-persistence”. This article provides a comprehensive review of the literature on FQs in water bodies, summarizing and analyzing contamination levels of FQs in global surface water over the past three years, as well as the bioaccumulation and metabolism patterns of FQs in aquatic organisms, their ecological toxicity, and the influencing factors. The results show that FQs contamination is widespread in surface water across the surveyed 32 countries, with ciprofloxacin and norfloxacin being the most heavy contaminants. Furthermore, contamination levels are generally higher in developing and developed countries. It has been observed that compound types, species, and environmental factors influence the bioaccumulation, metabolism, and toxicity of FQs in aquatic organisms. FQs tend to accumulate more in organisms with higher lipid content, and toxicity experiments have shown that FQs exhibit the highest toxicity to bacteria and the weakest toxicity to mollusk. This article summarizes and analyzes the current research status and shortcomings of FQs, providing guidance and theoretical support for future research directions.

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Hierarchically-structured laccase@Ni3(PO4)2 hybrid nanoflowers for antibiotic degradation: Application in real wastewater effluent and toxicity evaluation

Cited by 9 publications

References 99 publications

Immobilized Dipeptidase in Manganese Ion-Loaded Polyethylenimine-Induced Calcium Phosphate Nanocrystals for Carnosine Synthesis

Immobilized Dipeptidase in Manganese Ion-Loaded Polyethylenimine-Induced Calcium Phosphate Nanocrystals for Carnosine Synthesis

Co-Immobilization of Laccase and Mediator into Fe-Doped ZIF-8 Significantly Enhances the Degradation of Organic Pollutants

Occurrence, Bioaccumulation, Metabolism and Ecotoxicity of Fluoroquinolones in the Aquatic Environment: A Review

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