Herbal Medicine from Single Clove Garlic Oil Extract Ameliorates Hepatic Steatosis and Oxidative Status in High Fat Diet Mice

Arifah, Siti Nur; Atho'illah, Mochammad Fitri; Lukiati, Betty; Lestari, Sri Rahayu

doi:10.21315/mjms2020.27.1.5

Cited by 23 publications

(24 citation statements)

References 31 publications

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“…TNF-α has a strong proinflammatory activity, whereas IFN-γ induce local chemokine to recruit other immune cells, including increasing T-cells infiltration to tissues [ 33 ]. In line with our previous study, SGO treatment also reduce TNF- α concentration in serum [ 34 ]. Furthermore, SGO restores the regulatory T-cells (Tregs) and its anti-inflammatory cytokine IL-10 and TGF-β [ 35 ].…”

Section: Discussionsupporting

confidence: 92%

Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet

Lestari

Atho'illah

Christina

et al. 2020

Journal of Ayurveda and Integrative Medicine

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Background Hyperlipidemia triggers atherosclerosis by involving immune cells, such as T-cells. T-cells plays a role in worsening conditions during a high-fat diet (HFD). Objective The research aimed to analyze the role of single garlic oil (SGO) on T-cells activation and its proinflammatory cytokine expression in HFD mice. Methods Mice were divided into six groups: ND (normal diet); HFD (high-fat diet without treatment); HFD + Simv (HFD + simvastatin 2.6 mg/kg body weight); and HFD + SGO 1–3 (high-fat diet + single garlic oil in a dose of 12.5, 25, and 50 mg/kg body weight), respectively. Treatments were orally given every day for 45 days. At the end of treatments, lymphocytes were isolated from mice spleen. The relative number of T-cells and proinflammatory cytokines were measured using flow-cytometry and analyzed using one-way ANOVA (p < 0.05). Result Our result indicated that HFD mice had lower naive T cells (CD4+CD62L+) than normal mice (p < 0.05). SGO treatment in HFD mice increased the relative number of naïve T cells. HFD treatment increased the expression of TNF-α and IFN-γ through NF-κB expression. Furthermore, SGO treatment improved the expression of TNF-α and IFN-γ. Conclusion Our study suggests that SGO could act as a promising prospect for therapy to improve chronic inflammation in a HFD.

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Section: Discussionsupporting

confidence: 92%

Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet

Lestari

Atho'illah

Christina

et al. 2020

Journal of Ayurveda and Integrative Medicine

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“…Based on that, dysregulation of TLRs activity could develop the risk of chronic inflammatory and immune disease. Recent studies indicate that phytochemicals based on natural products possessed an anti−inflammatory effect [ 28 , 29 ], make it possible to target TLRs and their downstream component [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Elicited soybean extract attenuates proinflammatory cytokines expression by modulating TLR3/TLR4 activation in high−fat, high−fructose diet mice

Atho'illah

Safitri²,

Nur’aini

et al. 2021

Journal of Ayurveda and Integrative Medicine

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Background The high−fat, high−fructose diet (HFFD) provokes overnutrition and inflammation directly, mainly through Toll−like receptors (TLRs). Soybean ( Glycine max L.) contains isoflavone that can be transformed into glyceollin by microbial and physical stimuli. Glyceollin possesses many beneficial effects on health. Objective This study evaluates the beneficial effect of soybean extract elicited by Saccharomyces cerevisiae and light (ESE) on dendritic cells (DCs) profile and naïve T cells in HFFD mice. Materials and methods Female Balb/C mice were fed with HFFD for 24 weeks then orally administered with simvastatin 2.8 mg/kg BW or ESE 78, 104, and 130 mg/kg BW at the last four weeks. The expression of splenic CD11c + TLR3 + , CD11c + TLR4 + , NFκB + , CD11c + IL-17 + , CD11c + TNF−α + , CD4 + CD62L + , and CD8 + CD62L + subsets was measured by flow cytometry. The molecular docking has been measured using Pyrx 0.8, displayed in PyMol and Biovia Discovery Studio. Result HFFD significantly increased CD11c + TLR3 + , CD11c + TLR4 + , NFκB + , CD11c + IL-17 + , CD11c + TNF−α + expression and decreased CD4 + CD62L + and CD8 + CD62L + ( p < 0.05) compared to normal diet (ND) groups. ESE reduced CD11c + TLR3 + , CD11c + TLR4 + , thereby decreasing NFκB + , as well as decreased the CD11c + IL−17 + , CD11c + TNF−α + , and restores CD4 + CD62L + and CD8 + CD62L + subsets in HFFD mice. Glyceollin II exhibited the best binding affinity with an average energy of −7.3 kcal/mol to TLR3 and −7.9 kcal/mol to TLR4. Conclusion The bioactive compound in ESE act synergistically to modulate TLR3/TLR4 activation, reduced NFκB, IL−17, and TNF−α, and restores naïve T cells expression in HFFD mice. ESE was a favorable candidate to mitigate chronic inflammation.

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“…The normal group was given a normal diet, while the control and treatment groups were given a high-fat diet (HFD) and 10% sucrose drinking water for 5 weeks. The HFD contained 8.84% carbohydrates, 8.5% protein, and 34.2% fat, with the remaining contents consisting of fiber, minerals, and vitamins [ 18 ]. HFD-fed rats were injected intraperitoneally with STZ in multiple, low doses (30 mg/kg body weight [BW], diluted in citrate-buffered saline pH 4.5).…”

Section: Methodsmentioning

confidence: 99%

Black soybean tempeh and purple sweet potato improve sperm quality in streptozotocin-induced diabetic rats

et al. 2020

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Background and Aim: Hyperglycemia increases advanced glycation end-product (AGE) production, and the activity of receptor for AGE (RAGE) in testis, which leads to testicular histopathological damage and infertility. This research investigated the effect of black soybean tempeh (BST), purple sweet potato (PSP), and its combination on AGE and RAGE expression and spermatozoa quality in streptozotocin (STZ)-induced diabetic rats. Materials and Methods: The rats were given high-fat diets for 5 weeks, then were injected intraperitoneally with multiple low doses of STZ (30 mg/kg body weight). Diabetes mellitus (DM) rats were divided into seven groups: DM, DM+glibenclamide, DM+BST, DM+PSP, and DM+combination of BST and PSP in ratio 1:3, 2:2, and 3:1 as C1, C2, and C3, respectively. The rats were treated for 30 days. Testicular AGE and RAGE expression and spermatozoa quality were measured. Results: The combination of BST and PSP significantly decreased AGE and RAGE expression in testicular organs and improved spermatozoa quality compared to the normal group. Conclusion: The combination of BST and PSP can be used as future alternatives to improve spermatozoa quality in DM patients.

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Herbal Medicine from Single Clove Garlic Oil Extract Ameliorates Hepatic Steatosis and Oxidative Status in High Fat Diet Mice

Cited by 23 publications

References 31 publications

Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet

Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet

Elicited soybean extract attenuates proinflammatory cytokines expression by modulating TLR3/TLR4 activation in high−fat, high−fructose diet mice

Black soybean tempeh and purple sweet potato improve sperm quality in streptozotocin-induced diabetic rats

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